Tal Effectors

Transcription activator-like effector nuclease

Structural insights into the specific recognition of 5-methylcytosine and 5-hydroxymethylcytosine by TAL effectors.

Transcription activator-like effectors (TALEs) acknowledge DNA by means of repeat-variable diresidues (RVDs) and TALE-DNA interactions are delicate to DNA modifications. Our earlier research deciphered the recognition of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) by TALEs. Here, we report seven crystal buildings of TALE-DNA complexes. The 5mC-specific RVD HA acknowledges 5mC by means of van der Waals interactions and displays...