The DNA damage response kinases DNA-dependent protein kinase (DNA-PK) and ataxia telangiectasia mutated (ATM) Are stimulated by bulky adduct-containing DNA.

The DNA damage response kinases DNA-dependent protein kinase (DNA-PK) and ataxia telangiectasia mutated (ATM) Are stimulated by bulky adduct-containing DNA.

A wide range of environmental, carcinogenic, and chemotherapeutic brokers kind bulky lesions on DNA that activate DNA damage checkpoint signaling pathways in human cells. To establish the mechanisms by which bulky DNA adducts induce damage signaling, we developed an in vitro assay utilizing mammalian cell nuclear extract and plasmid DNA containing bulky adducts shaped by N-acetoxy-2-acetylaminofluorene or benzo(a)pyrene diol epoxide. Using this cell-free …

The DNA damage response kinases DNA-dependent protein kinase (DNA-PK) and ataxia telangiectasia mutated (ATM) Are stimulated by bulky adduct-containing DNA. Read More »