Transcription activator-like effectors (TALEs) are proteins discovered within the genus Xanthomonas of phytopathogenic micro organism. These proteins enter the nucleus of cells within the host plant and can induce the expression of susceptibility genes (S genes), triggering illness. TALEs bind the promoter area of S genes following a particular code, which permits the prediction of binding websites based mostly on TALEs amino acid sequences.
New candidate S genes can then be found by discovering the intersection between genes induced within the presence of TALEs and genes containing predicted effector binding components. By contrasting differential expression data and binding website predictions throughout totally different datasets, patterns of TALE diversification or convergence could also be unveiled, however this requires the seamless integration of various genomic and transcriptomic data.
With this in thoughts, we current daTALbase, a curated relational database that integrates TALE-related data together with bacterial TALE sequences, plant promoter sequences, predicted TALE binding websites, transcriptomic data of host crops in response to TALE-harboring micro organism, and different related data. The database might be explored to uncover new candidate S genes in addition to to research variation in TALE repertories and their corresponding targets. The first model of the database right here offered consists of data for Oryza sp.-Xanthomonas pv. oryzae interactions. Future variations of the database will incorporate info for different pathosystems involving TALEs.
The impact of accelerating numbers of repeats on TAL effector DNA binding specificity.
Transcription activator-like effectors (TALEs) acknowledge their DNA targets by way of tandem repeats, every specifying a single nucleotide base in a one-to-one sequential association. Due to this modularity and their capacity to bind lengthy DNA sequences with excessive specificity, TALEs have been utilized in many functions. Contributions of particular person repeat-nucleotide associations to affinity and specificity have been characterised.
Here, utilizing in vitro binding assays, we examined the connection between the variety of repeats in a TALE and its affinity, for each goal and non-target DNA. Each extra repeat offers additional binding power for the goal DNA, with the acquire decaying exponentially such that binding power saturates. Affinity for non-target DNA additionally will increase non-linearly with the variety of repeats, however with a slower decay of acquire.
The distinction between the impact of size on affinity for goal versus non-target DNA manifests in specificity growing then diminishing with growing TALE size, peaking between 15 and 19 repeats. Modeling throughout totally different hypothetical saturation ranges and charges of acquire decay, reflecting totally different repeat compositions, yielded a comparable vary of specificity optima. This vary encompasses the imply and median size of native TALEs, suggesting that these proteins as a group have developed for most specificity.
Targeted promoter enhancing for rice resistance to Xanthomonas oryzae pv. oryzae reveals differential actions for SWEET14-inducing TAL effectors.
As a key virulence technique to trigger bacterial leaf blight, Xanthomonas oryzae pv. oryzae (Xoo) injects into the plant cell DNA-binding proteins referred to as transcription activator-like effectors (TALEs) that bind to effector-binding components (EBEs) in a sequence-specific method, leading to host gene induction. TALEs AvrXa7, PthXo3, TalC and Tal5, present in geographically distant Xoo strains, all goal OsSWEET14, thus thought of as a pivotal TALE goal performing as main susceptibility issue throughout rice-Xoo interactions.
Here, we report the technology of an allele library of the OsSWEET14 promoter via steady expression of TALE-nuclease (TALEN) constructs in rice. The susceptibility stage of traces carrying mutations in AvrXa7, Tal5 or TalC EBEs was assessed. Plants edited in AvrXa7 or Tal5 EBEs have been resistant to bacterial strains counting on the corresponding TALE. Surprisingly, though indels inside TalC EBE prevented OsSWEET14 induction in response to BAI3 wild-type micro organism counting on TalC, lack of TalC responsiveness failed to confer resistance to this pressure.
The TalC EBE mutant line was, nevertheless, resistant to a pressure expressing a synthetic SWEET14-inducing TALE whose EBE was additionally edited on this line. This work gives the primary set of alleles edited in TalC EBE and uncovers a distinct, broader vary of actions for TalC in contrast to AvrXa7 or Tal5. We suggest the existence of extra targets for TalC past SWEET14, suggesting that TALE-mediated plant susceptibility might consequence from induction of a number of, genetically redundant, host susceptibility genes by a single effector.
Deciphering TAL effectors for 5-methylcytosine and 5-hydroxymethylcytosine recognition.
DNA recognition by transcription activator-like effector (TALE) proteins is mediated by tandem repeats that specify nucleotides via repeat-variable diresidues. These repeat-variable diresidues type direct and sequence-specific contacts to DNA bases; therefore, TALE-DNA interplay is delicate to DNA chemical modifications. Here we conduct a thorough investigation, overlaying all theoretical repeat-variable diresidue combos, for their recognition capabilities for 5-methylcytosine and 5-hydroxymethylcytosine, two vital epigenetic markers in greater eukaryotes.
We determine each particular and degenerate repeat-variable diresidues for 5-methylcytosine and 5-hydroxymethylcytosine. Utilizing these novel repeat-variable diresidues, we obtain methylation-dependent gene activation and genome enhancing in vivo; we additionally report base-resolution detection of 5hmC in an in vitro assay.
NEIL3 Antibody |
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| CSB-PA065468-100ul | Cusabio | 100ul | 316 EUR |
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Description: A polyclonal antibody against NEIL3. Recognizes NEIL3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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NEIL3 Antibody |
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| 1-CSB-PA015695GA01HU | Cusabio |
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Description: A polyclonal antibody against NEIL3. Recognizes NEIL3 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
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NEIL3 Antibody |
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| DF4042 | Affbiotech | 200ul | 304 EUR |
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Description: NEIL3 Antibody detects endogenous levels of total NEIL3. |
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NEIL3 Antibody |
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| ABD4042 | Lifescience Market | 100 ug | 438 EUR |
NEIL3 antibody |
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| 10R-1522 | Fitzgerald | 100 ug | 512 EUR |
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Description: Mouse monoclonal NEIL3 antibody |
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NEIL3 antibody |
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| 70R-18837 | Fitzgerald | 50 ul | 435 EUR |
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Description: Rabbit polyclonal NEIL3 antibody |
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NEIL3 antibody |
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| 70R-36850 | Fitzgerald | 100 ug | 327 EUR |
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Description: Rabbit Polyclonal NEIL3 antibody |
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NEIL3 antibody |
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| 70R-51024 | Fitzgerald | 100 ul | 244 EUR |
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Description: Purified Polyclonal NEIL3 antibody |
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Mouse Nei Endonuclease VIII Like Protein 3 (NEIL3) ELISA Kit |
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| RD-NEIL3-Mu-48Tests | Reddot Biotech | 48 Tests | 577 EUR |
Mouse Nei Endonuclease VIII Like Protein 3 (NEIL3) ELISA Kit |
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| RD-NEIL3-Mu-96Tests | Reddot Biotech | 96 Tests | 802 EUR |
Mouse Nei Endonuclease VIII Like Protein 3 (NEIL3) ELISA Kit |
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| DLR-NEIL3-Mu-48T | DL Develop | 48T | 566 EUR |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Nei Endonuclease VIII Like Protein 3 (NEIL3) in samples from tissue homogenates, cell lysates or other biological fluids. |
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Mouse Nei Endonuclease VIII Like Protein 3 (NEIL3) ELISA Kit |
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| DLR-NEIL3-Mu-96T | DL Develop | 96T | 741 EUR |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Nei Endonuclease VIII Like Protein 3 (NEIL3) in samples from tissue homogenates, cell lysates or other biological fluids. |
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Mouse Nei Endonuclease VIII Like Protein 3 (NEIL3) ELISA Kit |
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| RDR-NEIL3-Mu-48Tests | Reddot Biotech | 48 Tests | 603 EUR |
Mouse Nei Endonuclease VIII Like Protein 3 (NEIL3) ELISA Kit |
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| RDR-NEIL3-Mu-96Tests | Reddot Biotech | 96 Tests | 840 EUR |
NEIL3 Polyclonal Antibody |
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| ES2917-100ul | ELK Biotech | 100ul | 279 EUR |
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Description: A Rabbit Polyclonal antibody against NEIL3 from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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NEIL3 Polyclonal Antibody |
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| ES2917-50ul | ELK Biotech | 50ul | 207 EUR |
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Description: A Rabbit Polyclonal antibody against NEIL3 from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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NEIL3 Polyclonal Antibody |
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| ABP51918-003ml | Abbkine | 0.03ml | 158 EUR |
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Description: A polyclonal antibody for detection of NEIL3 from Human, Mouse. This NEIL3 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human NEIL3 at AA range: 460-540 |
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NEIL3 Polyclonal Antibody |
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| ABP51918-01ml | Abbkine | 0.1ml | 289 EUR |
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Description: A polyclonal antibody for detection of NEIL3 from Human, Mouse. This NEIL3 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human NEIL3 at AA range: 460-540 |
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NEIL3 Polyclonal Antibody |
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| ABP51918-02ml | Abbkine | 0.2ml | 414 EUR |
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Description: A polyclonal antibody for detection of NEIL3 from Human, Mouse. This NEIL3 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human NEIL3 at AA range: 460-540 |
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Anti-NEIL3 antibody |
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| STJ94396 | St John's Laboratory | 200 µl | 197 EUR |
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Description: Rabbit polyclonal to NEIL3. |
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NEIL3 Polyclonal Antibody |
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| 41212-100ul | SAB | 100ul | 252 EUR |
NEIL3 Polyclonal Antibody |
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| 41212-50ul | SAB | 50ul | 187 EUR |
anti- NEIL3 antibody |
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| FNab05649 | FN Test | 100µg | 585 EUR |
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Description: Antibody raised against NEIL3 |
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Anti-NEIL3 antibody |
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| PAab05649 | Lifescience Market | 100 ug | 412 EUR |
NEIL3 siRNA |
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| 20-abx925706 | Abbexa |
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NEIL3 siRNA |
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| 20-abx925707 | Abbexa |
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Anti-NEIL3/Nei3 Antibody |
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| A05497-1 | BosterBio | 100ul | 397 EUR |
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Description: Rabbit Polyclonal Antibody for NEIL3 Antibody (NEIL3) detection.tested for WB in Human, Mouse. |
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NEIL3 Polyclonal Conjugated Antibody |
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| C41212 | SAB | 100ul | 397 EUR |
Human NEIL3 shRNA Plasmid |
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| 20-abx960591 | Abbexa |
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NEIL3 ELISA KIT|Human |
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| EF005329 | Lifescience Market | 96 Tests | 689 EUR |
NEIL3 Recombinant Protein (Human) |
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| RP078087 | ABM | 100 ug | Ask for price |
NEIL3 Blocking Peptide |
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| 20-abx063899 | Abbexa |
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NEIL3 Blocking Peptide |
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| DF4042-BP | Affbiotech | 1mg | 195 EUR |
NEIL3 ELISA Kit (Human) (OKEH05107) |
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| OKEH05107 | Aviva Systems Biology | 96 Wells | 727 EUR |
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Description: Description of target: DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.417 ng/mL |
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NEIL3 ORF Vector (Human) (pORF) |
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| ORF026030 | ABM | 1.0 ug DNA | 405 EUR |
Mouse NEIL3 shRNA Plasmid |
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| 20-abx982114 | Abbexa |
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NEIL3 Recombinant Protein (Rat) |
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| RP213656 | ABM | 100 ug | Ask for price |
NEIL3 Recombinant Protein (Mouse) |
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| RP153635 | ABM | 100 ug | Ask for price |
NEIL3 sgRNA CRISPR Lentivector set (Human) |
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| K1416101 | ABM | 3 x 1.0 ug | 339 EUR |
NEIL3 ELISA Kit (Mouse) (OKCD09389) |
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| OKCD09389 | Aviva Systems Biology | 96 Wells | 936 EUR |
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Description: Description of target: DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.6 Publications;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 0.061ng/mL |
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NEIL3 ELISA Kit (Mouse) (OKEH03545) |
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| OKEH03545 | Aviva Systems Biology | 96 Wells | 727 EUR |
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Description: Description of target: DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.6 Publications;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.418 ng/mL |
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Neil3 ORF Vector (Rat) (pORF) |
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| ORF071220 | ABM | 1.0 ug DNA | 506 EUR |
Neil3 ORF Vector (Mouse) (pORF) |
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| ORF051213 | ABM | 1.0 ug DNA | 506 EUR |
NEIL3 sgRNA CRISPR Lentivector (Human) (Target 1) |
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| K1416102 | ABM | 1.0 ug DNA | 154 EUR |
NEIL3 sgRNA CRISPR Lentivector (Human) (Target 2) |
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| K1416103 | ABM | 1.0 ug DNA | 154 EUR |
NEIL3 sgRNA CRISPR Lentivector (Human) (Target 3) |
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| K1416104 | ABM | 1.0 ug DNA | 154 EUR |
NEIL3 Protein Vector (Human) (pPB-C-His) |
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| PV104118 | ABM | 500 ng | 552 EUR |
NEIL3 Protein Vector (Human) (pPB-N-His) |
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| PV104119 | ABM | 500 ng | 552 EUR |
NEIL3 Protein Vector (Human) (pPM-C-HA) |
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| PV104120 | ABM | 500 ng | 552 EUR |
NEIL3 Protein Vector (Human) (pPM-C-His) |
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| PV104121 | ABM | 500 ng | 552 EUR |
Nei Endonuclease VIII Like Protein 3 (NEIL3) Antibody |
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| 20-abx129922 | Abbexa |
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Nei Endonuclease VIII Like Protein 3 (NEIL3) Antibody |
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| 20-abx177722 | Abbexa |
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Nei Endonuclease VIII Like Protein 3 (NEIL3) Antibody |
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| abx331074-100ul | Abbexa | 100 ul | 425 EUR |
Nei Endonuclease VIII Like Protein 3 (NEIL3) Antibody |
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| 20-abx325929 | Abbexa |
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Human NEIL3/ Endonuclease 8-like 3 ELISA Kit |
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| E1730Hu | Sunlong | 1 Kit | 605 EUR |
Human Endonuclease 8- like 3, NEIL3 ELISA KIT |
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| ELI-45663h | Lifescience Market | 96 Tests | 824 EUR |
Neil3 sgRNA CRISPR Lentivector set (Rat) |
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| K6401001 | ABM | 3 x 1.0 ug | 339 EUR |
Neil3 sgRNA CRISPR Lentivector set (Mouse) |
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| K4227101 | ABM | 3 x 1.0 ug | 339 EUR |
Nei Endonuclease VIII-Like 3 (E. Coli) (NEIL3) Antibody |
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| 20-abx008114 | Abbexa |
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Nei Endonuclease VIII-Like 3 (E. Coli) (NEIL3) Antibody |
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| abx030990-400ul | Abbexa | 400 ul | 523 EUR |
Nei Endonuclease VIII-Like 3 (E. Coli) (NEIL3) Antibody |
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| abx030990-80l | Abbexa | 80 µl | 286 EUR |
Nei Endonuclease VIII-Like 3 (E. Coli) (NEIL3) Antibody |
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| 20-abx014459 | Abbexa |
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Nei Endonuclease VIII-Like 3 (E. Coli) (NEIL3) Antibody |
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| abx235649-100ug | Abbexa | 100 ug | 551 EUR |
Neil3 sgRNA CRISPR Lentivector (Rat) (Target 1) |
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| K6401002 | ABM | 1.0 ug DNA | 154 EUR |
Neil3 sgRNA CRISPR Lentivector (Rat) (Target 2) |
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| K6401003 | ABM | 1.0 ug DNA | 154 EUR |
Neil3 sgRNA CRISPR Lentivector (Rat) (Target 3) |
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| K6401004 | ABM | 1.0 ug DNA | 154 EUR |
Neil3 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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| K4227102 | ABM | 1.0 ug DNA | 154 EUR |
Neil3 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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| K4227103 | ABM | 1.0 ug DNA | 154 EUR |
Neil3 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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| K4227104 | ABM | 1.0 ug DNA | 154 EUR |
NEIL3 Protein Vector (Rat) (pPB-C-His) |
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| PV284878 | ABM | 500 ng | 603 EUR |
NEIL3 Protein Vector (Rat) (pPB-N-His) |
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| PV284879 | ABM | 500 ng | 603 EUR |
NEIL3 Protein Vector (Rat) (pPM-C-HA) |
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| PV284880 | ABM | 500 ng | 603 EUR |
NEIL3 Protein Vector (Rat) (pPM-C-His) |
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| PV284881 | ABM | 500 ng | 603 EUR |
NEIL3 Protein Vector (Mouse) (pPB-C-His) |
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| PV204850 | ABM | 500 ng | 603 EUR |
NEIL3 Protein Vector (Mouse) (pPB-N-His) |
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| PV204851 | ABM | 500 ng | 603 EUR |
NEIL3 Protein Vector (Mouse) (pPM-C-HA) |
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| PV204852 | ABM | 500 ng | 603 EUR |
NEIL3 Protein Vector (Mouse) (pPM-C-His) |
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| PV204853 | ABM | 500 ng | 603 EUR |
NEIL3 3'UTR GFP Stable Cell Line |
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| TU065564 | ABM | 1.0 ml | 1394 EUR |
Neil3 3'UTR Luciferase Stable Cell Line |
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| TU213880 | ABM | 1.0 ml | Ask for price |
Neil3 3'UTR GFP Stable Cell Line |
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| TU263880 | ABM | 1.0 ml | Ask for price |
NEIL3 3'UTR Luciferase Stable Cell Line |
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| TU015564 | ABM | 1.0 ml | 1394 EUR |
Neil3 3'UTR Luciferase Stable Cell Line |
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| TU113982 | ABM | 1.0 ml | Ask for price |
Neil3 3'UTR GFP Stable Cell Line |
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| TU163982 | ABM | 1.0 ml | Ask for price |
Nei Endonuclease VIII Like Protein 3 (NEIL3) Polyclonal Antibody (Mouse) |
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| 4-PAN291Mu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Nei Endonuclease VIII Like Protein 3 (NEIL3) |
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NEIL3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) |
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| K1416105 | ABM | 3 x 1.0 ug | 376 EUR |
Recombinant Nei Endonuclease VIII Like Protein 3 (NEIL3) |
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| 4-RPN291Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Nei Endonuclease VIII Like Protein 3 expressed in: E.coli |
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Mouse Neil3/ Endonuclease 8-like 3 ELISA Kit |
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| E1017Mo | Sunlong | 1 Kit | 632 EUR |
Mouse Neil3(Endonuclease 8-like 3) ELISA Kit |
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| EM0777 | FN Test | 96T | 524.1 EUR |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.469 ng/ml |
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Bovine Endonuclease 8- like 3, NEIL3 ELISA KIT |
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| ELI-23242b | Lifescience Market | 96 Tests | 928 EUR |
Mouse Endonuclease 8- like 3, Neil3 ELISA KIT |
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| ELI-45664m | Lifescience Market | 96 Tests | 865 EUR |
Nei Endonuclease VIII Like Protein 3 (NEIL3) Polyclonal Antibody (Mouse), APC |
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| 4-PAN291Mu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Nei Endonuclease VIII Like Protein 3 (NEIL3). This antibody is labeled with APC. |
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Nei Endonuclease VIII Like Protein 3 (NEIL3) Polyclonal Antibody (Mouse), Biotinylated |
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| 4-PAN291Mu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Nei Endonuclease VIII Like Protein 3 (NEIL3). This antibody is labeled with Biotin. |
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Our work deciphers repeat-variable diresidues for 5-methylcytosine and 5-hydroxymethylcytosine, and offers instruments for TALE-dependent epigenome recognition.Transcription activator-like effector proteins recognise particular DNA sequences by way of tandem repeats. Here the authors show TALEs can recognise the methylated bases 5mC and 5hmC, enabling them to detect epigenetic modifications.
