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Transcription activator-like effector nuclease

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Highly sensitive ELISA for UV-induced DNA damage

The High Sensitivity CPD and the High Sensitivity (6-4) photoproducts ELISA from Cosmo Bio

Most of the UV-induced DNA damage are cyclobutane pyrimidine dimers (CPDs) and (6-4) photoproducts (6-4PPs), which are formed between neighboring pyrimidine nucleotides on the same DNA strand. These helix-distorting DNA lesions are only repaired in humans by a nucleotide excision repair system.

The main types of UV-induced DNA damage are cyclobutane pyrimidine dimers (CPDs), (6-4) photo products (6-4PPs) and dewar photo products (DewarPPs), which are formed between neighboring pyrimidine nucleotides on the same DNA strand become.
Cyclobutane pyrimidine dimers (CPDs), (6-4) photoproducts (6-4PPs) and Dewar photoproducts (DewarPPs) are UV-induced DNA damage that are formed between neighboring pyrimidine nucleotides on the same DNA strand.

The High Sensitivity CPD / Cyclobutane Pyrimidine Dimer ELISA kit (CSR-NM-MA-K003) from Cosmo Bio is the only commercially available ELISA using the anti-CPD clone TDM-2 and was developed for the highly sensitive detection of CPDs in cultured cells or DNA purified from the skin epidermis.

The High Sensitivity (6-4) photoproducts (6-4PPs) ELISA Kit (CSR-NM-MA-K002) is the only commercially available ELISA that uses the anti-6-4PPs clone 64M-2 and for the highly sensitive one Detection of 6-4PPs from cultured cells from the skin epidermis was optimized.

This ELISA recognizes CPDs or 6-4PPs from dipyrimidines in all DNA sequence contexts (e.g. TT, TC, CT and CC). Therefore, the availability and convenience of this ELISA kit will help to understand molecular mechanisms of cellular responses to UV radiation and DNA damage with applications in many areas of research such as cancer research, photobiology, dermatology, ophthalmology, immunology and cosmetic science.

To better investigate molecular events in the environment of UV-induced DNA damage and repair, Mori et al. previously developed and characterized monoclonal antibodies (mAb) specifically for CPDs and mAb specifically for 6-4PPs (1), while Matsunaga et al. have developed and characterized mAb specifically for Dewar photo products (DewarPPs) (2).

Three of these antibodies (CPDs: clone TDM-2 [CAC-NM-DND-001]; 6-4PPs: clone 64M-2 [CAC-NM-DND-002]; DewarPPs: clone DEM-1 [CAC-NM-DND -003]) continue to be frequently cited in the literature, often for use in ELISA by a recommended method. These antibodies enable the quantification of photoproducts in purified DNA, those from cultured cells or from the skin epidermis using an enzyme-linked immunoadsorption test (ELISA), and the visualization and measurement of photoproducts in the DNA from cultured cells or the skin by means of indirect immunofluorescence (IIF).

References

1) Mori, T., et al., Photochem. Photobiol. 54, 225-232 (1991).

2) Matsunaga, T., et al., Photochem. Photobiol. 54, 403-410 (1991).

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