Sunday, 03 March, 2024

Tal Effectors

Transcription activator-like effector nuclease

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  • Multiple Chemical Inducible Tal Effectors for Genome Editing and Transcription Activation.
Multiple Chemical Inducible Tal Effectors for Genome Editing and Transcription Activation.
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Multiple Chemical Inducible Tal Effectors for Genome Editing and Transcription Activation.

Inducible modulation is usually required for exact investigations and manipulations of dynamic organic processes. Transcription activator-like effectors (TALEs) present a robust device for focused gene modifying and transcriptional programming. We designed a sequence of chemical inducible methods by coupling TALEs with a mutated human estrogen receptor (ERT2), which renders them 4-hydroxyl-tamoxifen (4-OHT) inducible for entry of the genome.
Chemical inducible genome modifying was achieved by way of fusing two tandem ERT2 domains to personalised transcription activator-like effector nuclease (TALEN), which we termed “Hybrid Inducible Technology” (HIT-TALEN). Those for transcription activation had been vigorously optimized utilizing a number of assemble designs. Most environment friendly drug induction for endogenous gene activation was achieved with minimal background exercise utilizing an optimized inducible TALE based mostly SunTag system (HIT-TALE-SunTag).
The HIT-SunTag system is fast, tunable, selective to 4-OHT over an endogenous ligand, and reversible in drug induced transcriptional activation. Versatile methods developed on this research may be simply utilized for modifying and transcriptional programming of doubtless any genomic loci in a good and efficient chemical inducible trend.

Xanthomonas TAL effectors hijack host basal transcription issue IIA α and γ subunits for invasion.

The Xanthomonas genus consists of Gram-negative plant-pathogenic micro organism, which infect a broad vary of crops and wild plant species, trigger signs with leaf blights, streaks, spots, stripes, necrosis, wilt, cankers and gummosis on leaves, stems and fruits in all kinds of crops by way of injecting their effector proteins into the host cell throughout an infection. Among these virulent effectors, transcription activator-like effectors (TALEs) work together with the γ subunit of host transcription issue IIA (TFIIAγ) to activate the transcription of host illness susceptibility genes.
Functional TFIIA is a ternary advanced comprising α, β and γ subunits. However, whether or not TALEs recruit TFIIAα, TFIIAβ, or each stays unknown. The underlying molecular mechanisms by which TALEs mediate host susceptibility gene activation require full elucidation. Here, we present that TALEs work together with the α+γ binary subcomplex however not the α+β+γ ternary advanced of rice TFIIA (holo-OsTFIIA). The transcription issue binding (TFB) areas of TALEs, that are extremely conserved in Xanthomonas species, have a dominant function in these interactions.
Furthermore, the interplay between TALEs and the α+γ advanced displays sturdy DNA binding exercise in vitro. These outcomes collectively reveal that TALE-carrying pathogens hijack the host basal transcription components TFIIAα and TFIIAγ, however not TFIIAβ, to reinforce host susceptibility throughout pathogen an infection. The uncovered mechanism widens new insights on host-microbe interplay and present an relevant technique to breed high-resistance crop varieties.

A Conserved Basal Transcription Factor Is Required for the Function of Diverse TAL Effectors in Multiple Plant Hosts.

Many Xanthomonas micro organism use transcription activator-like effector (TALE) proteins to activate plant illness susceptibility (S) genes, and this activation contributes to illness. We not too long ago reported that rice basal transcription issue IIA gamma subunit, OsTFIIAγ5, is hijacked by TALE-carrying Xanthomonas oryzae infecting the crops.
However, whether or not TFIIAγs are additionally concerned in TALE-carrying Xanthomonas-caused illnesses in different crops is unknown. Here, molecular and genetic approaches had been used to research the function of TFIIAγs in different crops. We discovered that TFIIAγs are additionally utilized by TALE-carrying Xanthomonas to trigger illness in different crops.
The TALEs of Xanthomonas citri pv. citri (Xcc) inflicting canker in citrus and Xanthomonas campestris pv. vesicatoria (Xcv) inflicting bacterial spot in pepper and tomato interacted with corresponding host TFIIAγs as in rice. Transcriptionally suppressing TFIIAγ led to resistance to Xcc in citrus and Xcv in pepper and tomato. The 39th residue of OsTFIIAγ5 and citrus CsTFIIAγ is significant for TALE-dependent induction of plant S genes.
As mutated OsTFIIAγ5V 39E, CsTFIIAγV 39E, pepper CaTFIIAγV 39E, and tomato SlTFIIAγV 39E additionally didn’t work together with TALEs to forestall illness. These outcomes counsel that TALE-carrying micro organism share a standard mechanism for infecting crops. Using TFIIAγV 39E-type mutation could possibly be a normal technique for bettering resistance to TALE-carrying pathogens in crops.
Multiple Chemical Inducible Tal Effectors for Genome Editing and Transcription Activation.

The TAL Effector AvrBs3 from Xanthomonas campestris pv. vesicatoria Contains Multiple Export Signals and Can Enter Plant Cells within the Absence of the Type III Secretion Translocon.

Pathogenicity of the Gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria depends upon a kind III secretion (T3S) system which translocates effector proteins into plant cells. Effector protein supply is managed by the T3S chaperone HpaB, which presumably escorts effector proteins to the secretion equipment.
One intensively studied effector is the transcription activator-like (TAL) effector AvrBs3, which binds to promoter sequences of plant goal genes and prompts plant gene expression. It was beforehand reported that kind III-dependent supply of AvrBs3 depends upon the N-terminal protein area. The indicators that management T3S and translocation of AvrBs3, nonetheless, haven’t but been characterised. In the current research, we present that T3S and translocation of AvrBs3 depend upon the N-terminal 10 and 50 amino acids, respectively.
Furthermore, we offer experimental proof that further indicators within the N-terminal 30 amino acids and the area between amino acids 64 and 152 promote translocation of AvrBs3 within the absence of HpaB. Unexpectedly, in vivo translocation assays revealed that AvrBs3 is delivered into plant cells even within the absence of HrpF, which is the anticipated channel-forming part of the T3S translocon within the plant plasma membrane.

Nuclease Free, Water, Ultra Pure Grade, 500ml

PB0100-500ml Vivantis each Ask for price

Water, Ultrapure, Nuclease Free, 0.1um Filtered, DNase, RNase and Protease Free

W0807-050 GenDepot 500 ml 40 EUR

Water, Ultrapure, Nuclease Free, 0.1um Filtered, DNase, RNase and Protease Free

W0807-100 GenDepot 1L 50 EUR

Nuclease-free H2O

RK20102 Abclonal 25mL 21.5 EUR

Nuclease-free Water

abx098144-100l Abbexa 100 µl 212.5 EUR

Nuclease-free Water

abx098144-1ml Abbexa 1 ml Ask for price

Nuclease-free Water

abx098144-200l Abbexa 200 µl Ask for price

Nuclease P1 rabbit polyclonal antibody, Azide Free

AP21351AF-N Origene Technologies GmbH 10 mg Ask for price

BioMag® Nuclease-Free Streptavidin Particles

8MB4804-1 Polysciences Europe GmbH 100ml 3034 EUR

BioMag® Nuclease-Free Streptavidin Particles

8MB4804-10 Polysciences Europe GmbH 10ml 326 EUR

BioMag® Nuclease-Free Streptavidin Particles

8MB4804-25 Polysciences Europe GmbH 25ml 892 EUR

BenzNuclease DNA and RNA Nuclease (Ultra Pure, Protease Free) Recombinant Protein

11-552 ProSci 50KU 481.2 EUR
Description: BenzNuclease DNA and RNA Nuclease (Ultra Pure, Protease Free) Recombinant Protein

Bacteria-Derived BenzNuclease DNA and RNA Nuclease (Ultra Pure, Protease Free) Recombinant Protein Tag Free Lyophilized

IBCTBZNCDNARNANUPPROFRTFLY50KU Innovative research each 535 EUR
Description: Bacteria-Derived BenzNuclease DNA and RNA Nuclease (Ultra Pure, Protease Free) Recombinant Protein Tag Free Lyophilized

Nuclease AWAY

A6257-100ML Biomatik Corporation 100ML 22 EUR
Description: Biotechnology

Nuclease AWAY

A6257-500ML Biomatik Corporation 500ML 88 EUR
Description: Biotechnology

Cas9 Nuclease

310 GeneOn 2x40µg (2x250 pmol) 316.8 EUR

Cas9 Nuclease

312HC GeneOn 80 µg (500 pmol) 268.8 EUR

Cas9 Nuclease

314HC GeneOn 400 µg (2500 pmol) 894 EUR

Cas9 Nuclease

EN301-01 Vazyme 50 pmol 146.4 EUR

Cas9 Nuclease

EN301-02 Vazyme 250 pmol 184.8 EUR

Ultra Nuclease

HBP000106 HZymes Biotechnology 20μL 14.73 EUR

Ultra Nuclease

HBP000107 HZymes Biotechnology 200μL 116.82 EUR

Ultra Nuclease

HBP000108 HZymes Biotechnology 2mL 965.04 EUR

Ultra Nuclease

HBP000109 HZymes Biotechnology 20mL 8634.56 EUR

Turbo Nuclease

EN-180L Jena Bioscience GmbH 5 x 10000units 384.5 EUR
Description: Serratia marcescens, recombinant, E. coli

Turbo Nuclease

EN-180S Jena Bioscience GmbH 10000units 96.2 EUR
Description: Serratia marcescens, recombinant, E. coli

Microccal Nuclease

TBS6020 Tribioscience 10KU 89 EUR

Nuclease P1 Antibody

20-abx300888 Abbexa
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Nuclease P1 Antibody

1-CSB-PA328459LA01PEU Cusabio
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Description: A polyclonal antibody against Nuclease P1. Recognizes Nuclease P1 from Penicillium citrinum. This antibody is Unconjugated. Tested in the following application: ELISA

Nuclease P1 Antibody

abx300888-100g Abbexa 100 µg 362.5 EUR

Nuclease P1 Antibody

abx300888-20g Abbexa 20 µg 162.5 EUR

Nuclease P1 Antibody

abx300888-50g Abbexa 50 µg 250 EUR

Benzonase Nuclease, 99%

enz-1112 ProSpec Tany 5000U 90 EUR
Description: Recombinant Serratia Marcescens Benzonase Nuclease, 99%

Benzonase Nuclease, 90%

enz-1150 ProSpec Tany 5000U 70 EUR
Description: Recombinant Serratia Marcescens Benzonase Nuclease, 90%

Cas9 Nuclease Protein

K008 ABM 8.0 µg (50pmol) Volume: 50µL 40 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).;;The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants.

saCas9 Nuclease Protein

K044 ABM 6.5 µg (50pmol) Volume: 50µL 55 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).;;The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT).

Cas9 Nuclease - 400µg - 1,600 ng/µl

3276-(HC) Intact Genomics 1/EA 678 EUR

Cas9 Nuclease Lentivirus

K003 ABM 300 µl, 10^7 IU/ml, Titer: 10^7 IU/ml 315 EUR
Description: N/A

Cas9 Nuclease Adenovirus

K004 ABM 1.0 ml, 10^6 pfu/ml, Titer: 10^6 pfu/ml 315 EUR
Description: N/A

Nuclease Mix 0.5mL - EACH

80650142 Scientific Laboratory Supplies EACH 191.7 EUR

Cas9 Nuclease - 80µg - 1,600 ng/µl

3273-(HC) Intact Genomics 1/EA 226.8 EUR

Nuclease P1 Antibody (HRP)

20-abx300889 Abbexa
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  • 100 ug
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Nuclease P1 Antibody (HRP)

abx300889-100g Abbexa 100 µg 362.5 EUR

Nuclease P1 Antibody (HRP)

abx300889-20g Abbexa 20 µg 162.5 EUR

Nuclease P1 Antibody (HRP)

abx300889-50g Abbexa 50 µg 250 EUR

Nuclease P1 Antibody (FITC)

20-abx300890 Abbexa
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  • 100 ug
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Cas9 Nuclease NLS Protein

K030 ABM 8.0 µg (50pmol) Volume: 50µL 40 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).;;The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants. Cas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.

Nuclease P1 Antibody (FITC)

abx300890-100g Abbexa 100 µg 362.5 EUR

Nuclease P1 Antibody (FITC)

abx300890-20g Abbexa 20 µg 162.5 EUR

Nuclease P1 Antibody (FITC)

abx300890-50g Abbexa 50 µg 250 EUR

Rabbit polyclonal antibody to Apoptosis-enhancing nuclease (apoptosis enhancing nuclease)

TA308608 Origene Technologies GmbH 100 µl Ask for price

Nuclease P1 Antibody (Biotin)

20-abx300891 Abbexa
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  • 100 ug
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fnCpf1 Nuclease NLS Protein

K087 ABM 38 μg (250 pmol) Volume: 25µL 75 EUR
Description: Using Cpf1 (a.k.a. Cas12a) in your CRISPR experiment offers several advantages over other CRISPR-associated nucleases.;
  • Due to the T-rich PAM sequence (TTTN), Cpf1 enables editing in regions unable to be targeted by Cas9.
  • Cpf1 can be used with a shorter guide RNA (called crRNA) than Cas9.
  • Cpf1 creates a staggered cut in dsDNA instead of a blunt cut.
  • Cpf1 cuts distal to the PAM sequence, which may allow for multiple rounds of cleavage.
    • fnCpf1 is from the bacteria Francisella novicida. This protein contains a SV40 T antigen nuclear localization signal (NLS) on the N-terminus of the protein. If the cut caused by fnCpf1 is repaired by non-homologous end joining (NHEJ), an indel may be formed that disrupts the open reading frame of the targeted gene, leading to gene knockout. Alternatively, by supplying a repair template, a sequence can be knocked in at the cleavage site via homology directed repair (HDR).

Nuclease P1 Antibody (Biotin)

abx300891-100g Abbexa 100 µg 362.5 EUR

Nuclease P1 Antibody (Biotin)

abx300891-20g Abbexa 20 µg 162.5 EUR

Nuclease P1 Antibody (Biotin)

abx300891-50g Abbexa 50 µg 250 EUR

Nuclease Decontamination Solution

43094006-1 Glycomatrix 250 mL 43.46 EUR

Universal Nuclease ELISA Kit

RK04660 Abclonal 96T 280 EUR

Recombinant Human APEX nuclease-1

7-02476 CHI Scientific 2µg Ask for price

Recombinant Human APEX nuclease-1

7-02477 CHI Scientific 10µg Ask for price

Recombinant Human APEX nuclease-1

7-02478 CHI Scientific 1mg Ask for price

APEX Nuclease-1 Human Recombinant

rAP-1339 Angio Proteomie Inquiry Ask for price

Nuclease P1 Polyclonal Antibody

A64318 EpiGentek
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  • 100 µg
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Cas9 Nuclease Lentiviral Vector

K002 ABM 10 ug 135 EUR
Description: N/A

Penicillium citrinum Nuclease P1

1-CSB-YP328459PEU Cusabio
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  • 100ug
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Description: Recombinant Penicillium citrinum Nuclease P1 expressed in Yeast

Penicillium citrinum Nuclease P1

1-CSB-EP328459PEU Cusabio
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  • 100ug
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  • 1MG
  • 200ug
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Description: Recombinant Penicillium citrinum Nuclease P1 expressed in E.coli

Penicillium citrinum Nuclease P1

1-CSB-EP328459PEUa0 Cusabio
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  • 100ug
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Description: Recombinant Penicillium citrinum Nuclease P1 expressed in E.coli

GENIUS™Nuclease DMF Filed

BEE-N3116 ACROBIOSYSTEMS 500KU 96.3 EUR
Description: GENIUS™Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-5 bases in length.

Cas9 Nuclease GFP NLS Protein

K048 ABM 9.5 µg (50pmol) Volume: 50µL 55 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR). ;The Cas9 from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. ;The fusion of Cas9 Nuclease NLS to GFP allows for visual confirmation of transfection as well as subsequent verification of Cas9 clearance from the cells. Cas9 Nuclease-GFP can also be used for FACS applications and screening. Cas9 Nuclease-GFP NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein. Our Cas9-GFP proteins have the Enhanced green fluorescent protein (eGFP).

Mitochondrial nuclease (NUC1) Antibody

abx345780-100l Abbexa 100 µl 350 EUR

Mitochondrial nuclease (NUC1) Antibody

abx345780-50l Abbexa 50 µl 250 EUR

Ultra Nuclease Assay Kit(ELISA)

HBP000111 HZymes Biotechnology 96 Tests 609.5 EUR

saCas9 Nuclease AAV Virus (AAV4)

K211 ABM 2 x 250 µl, 1 x 10^9 GC/ml, Titer: 1 x 10^9 GC/ml 375 EUR
Description: This AAV vector expresses the Cas9 orthologue from Staphylococcus Aureus (saCas9). saCas9 is ~1 kb shorter than spCas9, allowing it to be efficiently packaged in AAV Virus. Furthermore, the saCas9 enzyme recognizes a longer PAM sequence than spCas9, and thus has greater editing specificity.AAV has low immunogenicity and broad host range, making it an ideal choice for both in vivo and in vitro applications. Use this saCas9-expressing AAV virus with a target-specific saCas9-compatible sgRNA for highly specific and efficient genome editing.

saCas9 Nuclease AAV Virus (AAV5)

K212 ABM 2 x 250 µl, 1 x 10^9 GC/ml, Titer: 1 x 10^9 GC/ml 375 EUR
Description: This AAV vector expresses the Cas9 orthologue from Staphylococcus Aureus (saCas9). saCas9 is ~1 kb shorter than spCas9, allowing it to be efficiently packaged in AAV Virus. Furthermore, the saCas9 enzyme recognizes a longer PAM sequence than spCas9, and thus has greater editing specificity.AAV has low immunogenicity and broad host range, making it an ideal choice for both in vivo and in vitro applications. Use this saCas9-expressing AAV virus with a target-specific saCas9-compatible sgRNA for highly specific and efficient genome editing.

saCas9 Nuclease AAV Virus (AAV1)

K208 ABM 2 x 250 µl, 1 x 10^9 GC/ml, Titer: 1 x 10^9 GC/ml 375 EUR
Description: This AAV vector expresses the Cas9 orthologue from Staphylococcus Aureus (saCas9). saCas9 is ~1 kb shorter than spCas9, allowing it to be efficiently packaged in AAV Virus. Furthermore, the saCas9 enzyme recognizes a longer PAM sequence than spCas9, and thus has greater editing specificity.AAV has low immunogenicity and broad host range, making it an ideal choice for both in vivo and in vitro applications. Use this saCas9-expressing AAV virus with a target-specific saCas9-compatible sgRNA for highly specific and efficient genome editing.

saCas9 Nuclease AAV Virus (AAV2)

K209 ABM 2 x 250 µl, 1 x 10^9 GC/ml, Titer: 1 x 10^9 GC/ml 375 EUR
Description: This AAV vector expresses the Cas9 orthologue from Staphylococcus Aureus (saCas9). saCas9 is ~1 kb shorter than spCas9, allowing it to be efficiently packaged in AAV Virus. Furthermore, the saCas9 enzyme recognizes a longer PAM sequence than spCas9, and thus has greater editing specificity.AAV has low immunogenicity and broad host range, making it an ideal choice for both in vivo and in vitro applications. Use this saCas9-expressing AAV virus with a target-specific saCas9-compatible sgRNA for highly specific and efficient genome editing.

saCas9 Nuclease AAV Virus (AAV3)

K210 ABM 2 x 250 µl, 1 x 10^9 GC/ml, Titer: 1 x 10^9 GC/ml 375 EUR
Description: This AAV vector expresses the Cas9 orthologue from Staphylococcus Aureus (saCas9). saCas9 is ~1 kb shorter than spCas9, allowing it to be efficiently packaged in AAV Virus. Furthermore, the saCas9 enzyme recognizes a longer PAM sequence than spCas9, and thus has greater editing specificity.AAV has low immunogenicity and broad host range, making it an ideal choice for both in vivo and in vitro applications. Use this saCas9-expressing AAV virus with a target-specific saCas9-compatible sgRNA for highly specific and efficient genome editing.

saCas9 Nuclease AAV Virus (AAV6)

K213 ABM 2 x 250 µl, 1 x 10^9 GC/ml, Titer: 1 x 10^9 GC/ml 375 EUR
Description: This AAV vector expresses the Cas9 orthologue from Staphylococcus Aureus (saCas9). saCas9 is ~1 kb shorter than spCas9, allowing it to be efficiently packaged in AAV Virus. Furthermore, the saCas9 enzyme recognizes a longer PAM sequence than spCas9, and thus has greater editing specificity.AAV has low immunogenicity and broad host range, making it an ideal choice for both in vivo and in vitro applications. Use this saCas9-expressing AAV virus with a target-specific saCas9-compatible sgRNA for highly specific and efficient genome editing.

saCas9 Nuclease AAV Virus (AAV7)

K214 ABM 2 x 250 µl, 1 x 10^9 GC/ml, Titer: 1 x 10^9 GC/ml 375 EUR
Description: This AAV vector expresses the Cas9 orthologue from Staphylococcus Aureus (saCas9). saCas9 is ~1 kb shorter than spCas9, allowing it to be efficiently packaged in AAV Virus. Furthermore, the saCas9 enzyme recognizes a longer PAM sequence than spCas9, and thus has greater editing specificity.AAV has low immunogenicity and broad host range, making it an ideal choice for both in vivo and in vitro applications. Use this saCas9-expressing AAV virus with a target-specific saCas9-compatible sgRNA for highly specific and efficient genome editing.

saCas9 Nuclease AAV Virus (AAV8)

K215 ABM 2 x 250 µl, 1 x 10^9 GC/ml, Titer: 1 x 10^9 GC/ml 375 EUR
Description: This AAV vector expresses the Cas9 orthologue from Staphylococcus Aureus (saCas9). saCas9 is ~1 kb shorter than spCas9, allowing it to be efficiently packaged in AAV Virus. Furthermore, the saCas9 enzyme recognizes a longer PAM sequence than spCas9, and thus has greater editing specificity.AAV has low immunogenicity and broad host range, making it an ideal choice for both in vivo and in vitro applications. Use this saCas9-expressing AAV virus with a target-specific saCas9-compatible sgRNA for highly specific and efficient genome editing.

saCas9 Nuclease AAV Virus (AAV9)

K216 ABM 2 x 250 µl, 1 x 10^9 GC/ml, Titer: 1 x 10^9 GC/ml 375 EUR
Description: This AAV vector expresses the Cas9 orthologue from Staphylococcus Aureus (saCas9). saCas9 is ~1 kb shorter than spCas9, allowing it to be efficiently packaged in AAV Virus. Furthermore, the saCas9 enzyme recognizes a longer PAM sequence than spCas9, and thus has greater editing specificity.AAV has low immunogenicity and broad host range, making it an ideal choice for both in vivo and in vitro applications. Use this saCas9-expressing AAV virus with a target-specific saCas9-compatible sgRNA for highly specific and efficient genome editing.

anti-Apoptosis enhancing nuclease

YF-PA20583 Abfrontier 50 ug 435.6 EUR
Description: Mouse polyclonal to Apoptosis enhancing nuclease

saCas9 Nuclease AAV Virus (AAV11)

K218 ABM 2 x 250 µl, 1 x 10^9 GC/ml, Titer: 1 x 10^9 GC/ml 375 EUR
Description: This AAV vector expresses the Cas9 orthologue from Staphylococcus Aureus (saCas9). saCas9 is ~1 kb shorter than spCas9, allowing it to be efficiently packaged in AAV Virus. Furthermore, the saCas9 enzyme recognizes a longer PAM sequence than spCas9, and thus has greater editing specificity.AAV has low immunogenicity and broad host range, making it an ideal choice for both in vivo and in vitro applications. Use this saCas9-expressing AAV virus with a target-specific saCas9-compatible sgRNA for highly specific and efficient genome editing.

saCas9 Nuclease AAV Virus (AAV10)

K217 ABM 2 x 250 µl, 1 x 10^9 GC/ml, Titer: 1 x 10^9 GC/ml 375 EUR
Description: This AAV vector expresses the Cas9 orthologue from Staphylococcus Aureus (saCas9). saCas9 is ~1 kb shorter than spCas9, allowing it to be efficiently packaged in AAV Virus. Furthermore, the saCas9 enzyme recognizes a longer PAM sequence than spCas9, and thus has greater editing specificity.AAV has low immunogenicity and broad host range, making it an ideal choice for both in vivo and in vitro applications. Use this saCas9-expressing AAV virus with a target-specific saCas9-compatible sgRNA for highly specific and efficient genome editing.

Cas9 Nuclease Non-Viral Vector

K095 ABM 10 μg, Titer: N/A 135 EUR
Description: N/A

GENIUS™ Nuclease, premium grade

NUE-S5119 ACROBIOSYSTEMS 5000KU 1358.9 EUR
Description: GENIUS™ Nuclease, premium grade is a tag free recombinant form of Serratia marcescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™ Nuclease, premium grade is a homodimer with monomer molecular masses about 30 kDa with an isoelectric point (pI) at pH 6.85. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5’- phosphorylated oligonucleotides of 3-5 bases in length.

Apoptosis enhancing nuclease antibody

70R-13466 Fitzgerald 100 ul 550 EUR
Description: Affinity purified Rabbit polyclonal Apoptosis enhancing nuclease antibody

Rat APEX Nuclease 1 ELISA Kit

IRTAPEX1KT Innovative research each 790 EUR
Description: Rat APEX Nuclease 1 ELISA Kit

pSpCas9 nuclease & AAVS1 gRNA Vector

GA001 ALSTEM 10 μg 525 EUR

Apoptosis-enhancing nuclease antibody

22725 SAB 100ul 479 EUR

Apoptosis-enhancing nuclease antibody

22725-100ul SAB 100ul 468 EUR

NLS-Cas12a Nuclease (MALS verified)

CAA-L5149 ACROBIOSYSTEMS 100ug 107 EUR
Description: NLS-Cas12a Nuclease (CAA-L5149) is expressed from E. coli cells. It contains AA Ser 2 - His 1228 (Accession # QWN56480.1).

Nuclease P1 Antibody, HRP conjugated

1-CSB-PA328459LB01PEU Cusabio
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  • 100ug
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Description: A polyclonal antibody against Nuclease P1. Recognizes Nuclease P1 from Penicillium citrinum. This antibody is HRP conjugated. Tested in the following application: ELISA

Human APEX Nuclease 1 ELISA Kit

IHUAPEX1KT Innovative research each 733 EUR
Description: Human APEX Nuclease 1 ELISA Kit

Mouse APEX Nuclease 1 ELISA Kit

IMSAPEX1KT Innovative research each 752 EUR
Description: Mouse APEX Nuclease 1 ELISA Kit

GMP GENIUS™Nuclease DMF Filed

GMP-NUES19 ACROBIOSYSTEMS 5000KU 2011.6 EUR
Description: GMP GENIUS™Nuclease DMF Filed is a tag free recombinant form of Serratia marcescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GMP GENIUSNuclease DMF Filed is a homodimer with monomer molecular masses about 30 kDa with an isoelectric point (pI) at pH 6.85. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5’- phosphorylated oligonucleotides of 3-5 bases in length.

Nuclease P1 Antibody, FITC conjugated

1-CSB-PA328459LC01PEU Cusabio
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  • 100ug
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Description: A polyclonal antibody against Nuclease P1. Recognizes Nuclease P1 from Penicillium citrinum. This antibody is FITC conjugated. Tested in the following application: ELISA

Ultra Nuclease Assay Kit 2.0(ELISA)

HBP000116 HZymes Biotechnology 96 Tests 609.5 EUR

Fungus-Derived Nuclease S1 Purified

IFGS1NCL10KU Innovative research each 100 EUR
Description: Fungus-Derived Nuclease S1 Purified

Fungus-Derived Nuclease S1 Purified

IFGS1NCL50KU Innovative research each 247 EUR
Description: Fungus-Derived Nuclease S1 Purified

Cas9 Nuclease NLS Protein (Lyophilized)

K150 ABM 40µg (250pmol) 80 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants. Cas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.

Cas9 Nuclease NLS Protein (Lyophilized)

K151 ABM 200 µg (1.25 nmol) 295 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants. Cas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.

Nuclease P1 from Penicillium citrinum

OPNUCP1 Jaica 500 units/vial 545 EUR

Nuclease P1 Antibody, Biotin conjugated

1-CSB-PA328459LD01PEU Cusabio
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  • 100ug
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Description: A polyclonal antibody against Nuclease P1. Recognizes Nuclease P1 from Penicillium citrinum. This antibody is Biotin conjugated. Tested in the following application: ELISA

saCas9 Nuclease NLS Protein (Lyophilized)

K153 ABM 162.5 µg (1.25 nmol) 595 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT). saCas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.This saCas9 Nuclease NLS protein is offered as lyophilized powder for better long term storage and ambient temperature shipping. The protein is lyophilized as a low-salt powder, so it can be resuspended in your desired buffer for downstream applications.

saCas9 Nuclease NLS Protein (Lyophilized)

K152 ABM 32.5 µg (250pmol) 185 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT). saCas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.This saCas9 Nuclease NLS protein is offered as lyophilized powder for better long term storage and ambient temperature shipping. The protein is lyophilized as a low-salt powder, so it can be resuspended in your desired buffer for downstream applications.

Human Apoptosis-enhancing nuclease (AEN)

1-CSB-EP819883HU Cusabio
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Description: Recombinant Human Apoptosis-enhancing nuclease(AEN),partial expressed in E.coli

Cas9 Nuclease Protein (High Concentration)

K108 ABM 40 µg (250pmol) Volume: 25µL 80 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).;;The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants.

saCas9 Nuclease Protein (High Concentration)

K144 ABM 32.5 µg (250pmol) Volume: 25µL 155 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).;;The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT).

Rat APEX Nuclease 1 (APEX1) ELISA Kit

DLR-APEX1-Ra DL Develop 96T 499 EUR
Description: tissue homogenates, cell lysates or other biological fluids.

Rat APEX Nuclease 1 (APEX1) ELISA Kit

DLR-APEX1-Ra-48T DL Develop 48T 658.8 EUR
Description: A sandwich quantitative ELISA assay kit for detection of Rat APEX Nuclease 1 (APEX1) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat APEX Nuclease 1 (APEX1) ELISA Kit

DLR-APEX1-Ra-96T DL Develop 96T 861.6 EUR
Description: A sandwich quantitative ELISA assay kit for detection of Rat APEX Nuclease 1 (APEX1) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat APEX Nuclease 1 (APEX1) ELISA Kit

EK17390 SAB 96Т 768 EUR

Rat APEX Nuclease 1 (APEX1) ELISA Kit

DL-APEX1-Ra DL Develop 96T 475 EUR
Description: tissue homogenates, cell lysates or other biological fluids.

Rat APEX Nuclease 1 (APEX1) ELISA Kit

RD-APEX1-Ra-48T Reddot Biotech 48T 487.7 EUR
Description: tissue homogenates, cell lysates and other biological fluids.

Rat APEX Nuclease 1 (APEX1) ELISA Kit

RD-APEX1-Ra-48Tests Reddot Biotech 48 Tests 668.4 EUR

Rat APEX Nuclease 1 (APEX1) ELISA Kit

RD-APEX1-Ra-96T Reddot Biotech 96T 696.7 EUR
Description: tissue homogenates, cell lysates and other biological fluids.

Rat APEX Nuclease 1 (APEX1) ELISA Kit

RD-APEX1-Ra-96Tests Reddot Biotech 96 Tests 930 EUR

Rat APEX Nuclease 1 (APEX1) ELISA Kit

RDR-APEX1-Ra-48T Reddot Biotech 48T 512.09 EUR
Description: tissue homogenates, cell lysates and other biological fluids.

Rat APEX Nuclease 1 (APEX1) ELISA Kit

RDR-APEX1-Ra-48Tests Reddot Biotech 48 Tests 699.6 EUR

Rat APEX Nuclease 1 (APEX1) ELISA Kit

RDR-APEX1-Ra-96T Reddot Biotech 96T 731.54 EUR
Description: tissue homogenates, cell lysates and other biological fluids.

Rat APEX Nuclease 1 (APEX1) ELISA Kit

RDR-APEX1-Ra-96Tests Reddot Biotech 96 Tests 973.2 EUR

Ultra Nuclease Standard without His tag

HBP000105-1 HZymes Biotechnology 20μL 10.16 EUR

Ultra Nuclease Standard without His tag

HBP000105-2 HZymes Biotechnology 200μL 81.27 EUR

Ultra Nuclease Standard without His tag

HBP000105-3 HZymes Biotechnology 2mL 711.08 EUR

Ultra Nuclease Standard without His tag

HBP000105-4 HZymes Biotechnology 20mL 6094.99 EUR

GeneArt CRISPR Nuclease Vector- OFP- gRNA

PVT10418 Lifescience Market 2 ug 361.2 EUR

Apoptosis-Enhancing Nuclease (AEN) Antibody

20-abx308819 Abbexa
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Apoptosis-Enhancing Nuclease (AEN) Antibody

abx308819-100g Abbexa 100 µg 362.5 EUR

Apoptosis-Enhancing Nuclease (AEN) Antibody

abx308819-20g Abbexa 20 µg 162.5 EUR

Apoptosis-Enhancing Nuclease (AEN) Antibody

abx308819-50g Abbexa 50 µg 250 EUR

Recombinant Penicillium citrinum Nuclease P1

CSB-YP328459PEU Cusabio 6230 mg Ask for price

Recombinant Penicillium citrinum Nuclease P1

AP74245 SAB each Ask for price

Recombinant Penicillium citrinum Nuclease P1

CSB-EP328459PEU Cusabio 2951 mg Ask for price

Recombinant Penicillium citrinum Nuclease P1

CSB-EP328459PEUa0 Cusabio 2952 mg Ask for price

Recombinant Penicillium citrinum Nuclease P1

CSB-EP328459PEUb1 Cusabio 11327 mg Ask for price

Human APEX Nuclease 1 (APEX1) ELISA Kit

DLR-APEX1-Hu DL Develop 96T 463 EUR
Description: tissue homogenates, cell lysates or other biological fluids.

Human APEX Nuclease 1 (APEX1) ELISA Kit

DLR-APEX1-Hu-48T DL Develop 48T 620.4 EUR
Description: A sandwich quantitative ELISA assay kit for detection of Human APEX Nuclease 1 (APEX1) in samples from tissue homogenates, cell lysates or other biological fluids.

Human APEX Nuclease 1 (APEX1) ELISA Kit

DLR-APEX1-Hu-96T DL Develop 96T 807.6 EUR
Description: A sandwich quantitative ELISA assay kit for detection of Human APEX Nuclease 1 (APEX1) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse APEX Nuclease 1 (APEX1) ELISA Kit

DLR-APEX1-Mu DL Develop 96T 475 EUR
Description: tissue homogenates, cell lysates or other biological fluids.

Mouse APEX Nuclease 1 (APEX1) ELISA Kit

DLR-APEX1-Mu-48T DL Develop 48T 632.4 EUR
Description: A sandwich quantitative ELISA assay kit for detection of Mouse APEX Nuclease 1 (APEX1) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse APEX Nuclease 1 (APEX1) ELISA Kit

DLR-APEX1-Mu-96T DL Develop 96T 825.6 EUR
Description: A sandwich quantitative ELISA assay kit for detection of Mouse APEX Nuclease 1 (APEX1) in samples from tissue homogenates, cell lysates or other biological fluids.

Human APEX Nuclease 1 (APEX1) ELISA Kit

EKL55549-5x96T Biomatik Corporation 5x96T 3793.35 EUR

Human APEX Nuclease 1 (APEX1) ELISA Kit

EKL55549-96T Biomatik Corporation 96T 798.6 EUR

Human APEX Nuclease 1 (APEX1) ELISA Kit

EK17389 SAB 96Т 768 EUR

Human APEX Nuclease 1 (APEX1) ELISA Kit

DL-APEX1-Hu DL Develop 96T 441 EUR
Description: tissue homogenates, cell lysates or other biological fluids.

Mouse APEX Nuclease 1 (APEX1) ELISA Kit

DL-APEX1-Mu DL Develop 96T 453 EUR
Description: tissue homogenates, cell lysates or other biological fluids.

Mouse APEX Nuclease 1 (APEX1) ELISA Kit

EK12664 SAB 96Т 768 EUR

Human APEX Nuclease 2(APEX2)ELISA Kit

QY-E05142 Qayee Biotechnology 96T 433.2 EUR

Human APEX Nuclease 1(APEX1)ELISA Kit

QY-E05143 Qayee Biotechnology 96T 433.2 EUR
The presence of HpaB- and HrpF-independent transport routes means that the supply of AvrBs3 is initiated throughout early phases of the an infection course of, presumably earlier than the activation of HpaB or the insertion of the translocon into the plant plasma membrane.

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