Thursday, 18 July, 2024

Tal Effectors

Transcription activator-like effector nuclease

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  • Multiple Chemical Inducible Tal Effectors for Genome Editing and Transcription Activation.
Multiple Chemical Inducible Tal Effectors for Genome Editing and Transcription Activation.
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Multiple Chemical Inducible Tal Effectors for Genome Editing and Transcription Activation.

Inducible modulation is usually required for exact investigations and manipulations of dynamic organic processes. Transcription activator-like effectors (TALEs) present a robust device for focused gene modifying and transcriptional programming. We designed a sequence of chemical inducible methods by coupling TALEs with a mutated human estrogen receptor (ERT2), which renders them 4-hydroxyl-tamoxifen (4-OHT) inducible for entry of the genome.
Chemical inducible genome modifying was achieved by way of fusing two tandem ERT2 domains to personalised transcription activator-like effector nuclease (TALEN), which we termed “Hybrid Inducible Technology” (HIT-TALEN). Those for transcription activation had been vigorously optimized utilizing a number of assemble designs. Most environment friendly drug induction for endogenous gene activation was achieved with minimal background exercise utilizing an optimized inducible TALE based mostly SunTag system (HIT-TALE-SunTag).
The HIT-SunTag system is fast, tunable, selective to 4-OHT over an endogenous ligand, and reversible in drug induced transcriptional activation. Versatile methods developed on this research may be simply utilized for modifying and transcriptional programming of doubtless any genomic loci in a good and efficient chemical inducible trend.

Xanthomonas TAL effectors hijack host basal transcription issue IIA α and γ subunits for invasion.

The Xanthomonas genus consists of Gram-negative plant-pathogenic micro organism, which infect a broad vary of crops and wild plant species, trigger signs with leaf blights, streaks, spots, stripes, necrosis, wilt, cankers and gummosis on leaves, stems and fruits in all kinds of crops by way of injecting their effector proteins into the host cell throughout an infection. Among these virulent effectors, transcription activator-like effectors (TALEs) work together with the γ subunit of host transcription issue IIA (TFIIAγ) to activate the transcription of host illness susceptibility genes.
Functional TFIIA is a ternary advanced comprising α, β and γ subunits. However, whether or not TALEs recruit TFIIAα, TFIIAβ, or each stays unknown. The underlying molecular mechanisms by which TALEs mediate host susceptibility gene activation require full elucidation. Here, we present that TALEs work together with the α+γ binary subcomplex however not the α+β+γ ternary advanced of rice TFIIA (holo-OsTFIIA). The transcription issue binding (TFB) areas of TALEs, that are extremely conserved in Xanthomonas species, have a dominant function in these interactions.
Furthermore, the interplay between TALEs and the α+γ advanced displays sturdy DNA binding exercise in vitro. These outcomes collectively reveal that TALE-carrying pathogens hijack the host basal transcription components TFIIAα and TFIIAγ, however not TFIIAβ, to reinforce host susceptibility throughout pathogen an infection. The uncovered mechanism widens new insights on host-microbe interplay and present an relevant technique to breed high-resistance crop varieties.

A Conserved Basal Transcription Factor Is Required for the Function of Diverse TAL Effectors in Multiple Plant Hosts.

Many Xanthomonas micro organism use transcription activator-like effector (TALE) proteins to activate plant illness susceptibility (S) genes, and this activation contributes to illness. We not too long ago reported that rice basal transcription issue IIA gamma subunit, OsTFIIAγ5, is hijacked by TALE-carrying Xanthomonas oryzae infecting the crops.
However, whether or not TFIIAγs are additionally concerned in TALE-carrying Xanthomonas-caused illnesses in different crops is unknown. Here, molecular and genetic approaches had been used to research the function of TFIIAγs in different crops. We discovered that TFIIAγs are additionally utilized by TALE-carrying Xanthomonas to trigger illness in different crops.
The TALEs of Xanthomonas citri pv. citri (Xcc) inflicting canker in citrus and Xanthomonas campestris pv. vesicatoria (Xcv) inflicting bacterial spot in pepper and tomato interacted with corresponding host TFIIAγs as in rice. Transcriptionally suppressing TFIIAγ led to resistance to Xcc in citrus and Xcv in pepper and tomato. The 39th residue of OsTFIIAγ5 and citrus CsTFIIAγ is significant for TALE-dependent induction of plant S genes.
As mutated OsTFIIAγ5V 39E, CsTFIIAγV 39E, pepper CaTFIIAγV 39E, and tomato SlTFIIAγV 39E additionally didn’t work together with TALEs to forestall illness. These outcomes counsel that TALE-carrying micro organism share a standard mechanism for infecting crops. Using TFIIAγV 39E-type mutation could possibly be a normal technique for bettering resistance to TALE-carrying pathogens in crops.
Multiple Chemical Inducible Tal Effectors for Genome Editing and Transcription Activation.

The TAL Effector AvrBs3 from Xanthomonas campestris pv. vesicatoria Contains Multiple Export Signals and Can Enter Plant Cells within the Absence of the Type III Secretion Translocon.

Pathogenicity of the Gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria depends upon a kind III secretion (T3S) system which translocates effector proteins into plant cells. Effector protein supply is managed by the T3S chaperone HpaB, which presumably escorts effector proteins to the secretion equipment.
One intensively studied effector is the transcription activator-like (TAL) effector AvrBs3, which binds to promoter sequences of plant goal genes and prompts plant gene expression. It was beforehand reported that kind III-dependent supply of AvrBs3 depends upon the N-terminal protein area. The indicators that management T3S and translocation of AvrBs3, nonetheless, haven’t but been characterised. In the current research, we present that T3S and translocation of AvrBs3 depend upon the N-terminal 10 and 50 amino acids, respectively.
Furthermore, we offer experimental proof that further indicators within the N-terminal 30 amino acids and the area between amino acids 64 and 152 promote translocation of AvrBs3 within the absence of HpaB. Unexpectedly, in vivo translocation assays revealed that AvrBs3 is delivered into plant cells even within the absence of HrpF, which is the anticipated channel-forming part of the T3S translocon within the plant plasma membrane.

Water, Sterile, Nuclease Free

MBS635323-500mL MyBiosource 500mL 135 EUR

Water, Sterile, Nuclease Free

MBS635323-5x3L MyBiosource 5x3L 1090 EUR

Bovine Albumin Nuclease Free

IBOALBNF100MG Innovative research each 136 EUR
Description: Bovine Albumin Nuclease Free

Bovine Albumin Nuclease Free

IBOALBNF500MG Innovative research each 347 EUR
Description: Bovine Albumin Nuclease Free

Bovine Albumin Nuclease Free

MBS136206-100mg MyBiosource 100mg 235 EUR

Bovine Albumin Nuclease Free

MBS136206-500mg MyBiosource 500mg 450 EUR

Bovine Albumin Nuclease Free

MBS136206-5x500mg MyBiosource 5x500mg 1850 EUR

Nuclease Free, Water, Ultra Pure Grade, 500ml

PB0100-500ml Vivantis each Ask for price

Water, Sterile, Nuclease & Endotoxin Free

MBS635296-100mL MyBiosource 100mL 150 EUR

Water, Sterile, Nuclease & Endotoxin Free

MBS635296-1L MyBiosource 1L 380 EUR

Water, Sterile, Nuclease & Endotoxin Free

MBS635296-250mL MyBiosource 250mL 190 EUR

Water, Sterile, Nuclease & Endotoxin Free

MBS635296-500mL MyBiosource 500mL 255 EUR

Water, Sterile, Nuclease & Endotoxin Free

MBS635296-50mL MyBiosource 50mL 130 EUR

Water, Ultrapure, Nuclease Free, 0.1um Filtered, DNase, RNase and Protease Free

W0807-050 GenDepot 500 ml 40 EUR

Water, Ultrapure, Nuclease Free, 0.1um Filtered, DNase, RNase and Protease Free

W0807-100 GenDepot 1L 50 EUR

Nuclease-free H2O

RK20102 Abclonal 25mL 21.5 EUR

Water, nuclease-free

96370 Sisco Laboratories 5 x1 ml 2.48 EUR
Description: Part E

Water, nuclease-free

96370-1 Sisco Laboratories 100 ml 4.7 EUR
Description: Part E

Water, nuclease-free

96370-2 Sisco Laboratories 500 ml 14.63 EUR
Description: Part E

Nuclease-free Water

abx098144-100l Abbexa 100 µl 212.5 EUR

Nuclease-free Water

abx098144-1ml Abbexa 1 ml Ask for price

Nuclease-free Water

abx098144-200l Abbexa 200 µl Ask for price

Nuclease P1 rabbit polyclonal antibody, Azide Free

AP21351AF-N Origene Technologies GmbH 10 mg Ask for price

HARB1, NT (HARBI1, C11orf77, Putative nuclease HARBI1, Harbinger transposase-derived nuclease) (Azide free) (HRP)

MBS6305431-02mL MyBiosource 0.2mL 980 EUR

HARB1, NT (HARBI1, C11orf77, Putative nuclease HARBI1, Harbinger transposase-derived nuclease) (Azide free) (HRP)

MBS6305431-5x02mL MyBiosource 5x0.2mL 4250 EUR

Nuclease-free water, not DEPC treated

E0212-01 EURx 100ml 21.8 EUR
Description: For DNA targeted reactions such as PCR, ligation and restriction digestions

Nuclease-free water, not DEPC treated

E0212-02 EURx 1l 57.77 EUR
Description: For DNA targeted reactions such as PCR, ligation and restriction digestions

BioMag® Nuclease-Free Streptavidin Particles

8MB4804-1 Polysciences Europe GmbH 100ml 3034 EUR

BioMag® Nuclease-Free Streptavidin Particles

8MB4804-10 Polysciences Europe GmbH 10ml 326 EUR

BioMag® Nuclease-Free Streptavidin Particles

8MB4804-25 Polysciences Europe GmbH 25ml 892 EUR

BenzNuclease DNA and RNA Nuclease (Ultra Pure, Protease Free) Recombinant Protein

11-552 ProSci 50KU 481.2 EUR
Description: BenzNuclease DNA and RNA Nuclease (Ultra Pure, Protease Free) Recombinant Protein

MTMRF, NT (FAN1, KIAA1018, MTMR15, Fanconi-associated nuclease 1, FANCD2/FANCI-associated nuclease 1, Myotubularin-related protein 15) (Azide free) (HRP)

MBS6322839-02mL MyBiosource 0.2mL 980 EUR

MTMRF, NT (FAN1, KIAA1018, MTMR15, Fanconi-associated nuclease 1, FANCD2/FANCI-associated nuclease 1, Myotubularin-related protein 15) (Azide free) (HRP)

MBS6322839-5x02mL MyBiosource 5x0.2mL 4250 EUR

Bacteria-Derived BenzNuclease DNA and RNA Nuclease (Ultra Pure, Protease Free) Recombinant Protein Tag Free Lyophilized

IBCTBZNCDNARNANUPPROFRTFLY50KU Innovative research each 535 EUR
Description: Bacteria-Derived BenzNuclease DNA and RNA Nuclease (Ultra Pure, Protease Free) Recombinant Protein Tag Free Lyophilized

Bacteria-Derived BenzNuclease DNA and RNA Nuclease (Ultra Pure, Protease Free) Recombinant Protein Tag Free Lyophilized

MBS8430529-INQUIRE MyBiosource INQUIRE Ask for price

S1 Nuclease

E1335-01 EURx 10000U 43.6 EUR
Description: Non-specific endonuclease, active primarily on single-stranded DNA and RNA

S1 Nuclease

E1335-02 EURx 50000U 168.95 EUR
Description: Non-specific endonuclease, active primarily on single-stranded DNA and RNA

Nuclease AWAY

A6257-100ML Biomatik Corporation 100ML 22 EUR
Description: Biotechnology

Nuclease AWAY

A6257-500ML Biomatik Corporation 500ML 88 EUR
Description: Biotechnology

Cas9 Nuclease

310 GeneOn 2x40µg (2x250 pmol) 316.8 EUR

Cas9 Nuclease

312HC GeneOn 80 µg (500 pmol) 268.8 EUR

Cas9 Nuclease

314HC GeneOn 400 µg (2500 pmol) 894 EUR

Cas9 Nuclease

3273 Intact Genomics 80µg(1600ng/µl),500pmol 107.2 EUR

Cas9 Nuclease

3276 Intact Genomics 400µg(1600ng/µl),2500pmol 396 EUR

OMNI Nuclease

E1120-01 EURx 20000U 35.97 EUR
Description: Nonspecific nuclease that completely degrades all forms of DNA and RNA

OMNI Nuclease

E1120-02 EURx 100000U 162.41 EUR
Description: Nonspecific nuclease that completely degrades all forms of DNA and RNA

Cas9 Nuclease

EN301-01 Vazyme 50 pmol 146.4 EUR

Cas9 Nuclease

EN301-02 Vazyme 250 pmol 184.8 EUR

Ultra Nuclease

HBP000106 HZymes Biotechnology 20μL 14.73 EUR

Ultra Nuclease

HBP000107 HZymes Biotechnology 200μL 116.82 EUR

Ultra Nuclease

HBP000108 HZymes Biotechnology 2mL 965.04 EUR

Ultra Nuclease

HBP000109 HZymes Biotechnology 20mL 8634.56 EUR

Turbo Nuclease

EN-180L Jena Bioscience GmbH 5 x 10000units 384.5 EUR
Description: Serratia marcescens, recombinant, E. coli

Turbo Nuclease

EN-180S Jena Bioscience GmbH 10000units 96.2 EUR
Description: Serratia marcescens, recombinant, E. coli

Cas12a Nuclease

3373 Intact Genomics 80µg(1600ng/µl) 123.2 EUR

Cas12a Nuclease

3376 Intact Genomics 400µg(1600ng/µl) 420 EUR

Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase, NP1)

MBS653730-100Units MyBiosource 100Units 320 EUR

Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase, NP1)

MBS653730-250Units MyBiosource 250Units 635 EUR

Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase, NP1)

MBS653730-5x250Units MyBiosource 5x250Units 2710 EUR

Microccal Nuclease

TBS6020 Tribioscience 10KU 89 EUR

Benzonase Nuclease

MBS157420-100KUnits MyBiosource 100KUnits 320 EUR

Benzonase Nuclease

MBS157420-25KUnits MyBiosource 25KUnits 210 EUR

Benzonase Nuclease

MBS157420-50KUnits MyBiosource 50KUnits 245 EUR

Benzonase Nuclease

MBS157420-5x100KUnits MyBiosource 5x100KUnits 1115 EUR

APEX Nuclease I

MBS4380592-002mgWithBSAAzideat02mgmL MyBiosource 0.02mg(WithBSA&Azideat0.2mg/mL) 230 EUR

APEX Nuclease I

MBS4380592-01mgWithBSAAzideat02mgmL MyBiosource 0.1mg(WithBSA&Azideat0.2mg/mL) 405 EUR

APEX Nuclease I

MBS4380592-01mgWithoutBSAAzideat1mgmL MyBiosource 0.1mg(WithoutBSA&Azideat1mg/mL) 405 EUR

APEX Nuclease I

MBS4380592-5x01mgWithBSAAzideat02mgmL MyBiosource 5x0.1mg(WithBSA&Azideat0.2mg/mL) 1725 EUR

APEX Nuclease I

MBS4380592-5x01mgWithoutBSAAzideat1mgmL MyBiosource 5x0.1mg(WithoutBSA&Azideat1mg/mL) 1725 EUR

GENIUS™Nuclease

BEE-N3116-10KU ACROBIOSYSTEMS 10KU 98.1 EUR
Description: GENIUS™Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-5 bases in length.

GENIUS™Nuclease

BEE-N3116-500KU ACROBIOSYSTEMS 500KU 784.8 EUR
Description: GENIUS™Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-5 bases in length.

GENIUS™Nuclease

BEE-N3116-50KU ACROBIOSYSTEMS 50KU 196.2 EUR
Description: GENIUS™Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-5 bases in length.

Nuclease P1 Antibody

20-abx300888 Abbexa
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  • 100 ug
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  • 200 ug
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Nuclease P1 Antibody

1-CSB-PA328459LA01PEU Cusabio
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  • 100ug
  • 50ug
Description: A polyclonal antibody against Nuclease P1. Recognizes Nuclease P1 from Penicillium citrinum. This antibody is Unconjugated. Tested in the following application: ELISA

Nuclease P1 Antibody

abx300888-100g Abbexa 100 µg 362.5 EUR

Nuclease P1 Antibody

abx300888-20g Abbexa 20 µg 162.5 EUR

Nuclease P1 Antibody

abx300888-50g Abbexa 50 µg 250 EUR

Nuclease P1 Antibody

MBS7003563-005mg MyBiosource 0.05mg 190 EUR

Nuclease P1 Antibody

MBS7003563-01mg MyBiosource 0.1mg 270 EUR

Nuclease P1 Antibody

MBS7003563-5x01mg MyBiosource 5x0.1mg 1205 EUR

Benzonase Nuclease, 99%

enz-1112 ProSpec Tany 5000U 90 EUR
Description: Recombinant Serratia Marcescens Benzonase Nuclease, 99%

Benzonase Nuclease, 90%

enz-1150 ProSpec Tany 5000U 70 EUR
Description: Recombinant Serratia Marcescens Benzonase Nuclease, 90%

Cas9 Nuclease Protein

K008 ABM 8.0 µg (50pmol) Volume: 50µL 40 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).;;The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants.

Cas9 Nuclease Protein

MBS4156497-0008mg MyBiosource 0.008mg 200 EUR

Cas9 Nuclease Protein

MBS4156497-5x0008mg MyBiosource 5x0.008mg 595 EUR

Cas9 Nuclease Protein

MBS4156512-004mg MyBiosource 0.04mg 230 EUR

Cas9 Nuclease Protein

MBS4156512-5x004mg MyBiosource 5x0.04mg 710 EUR

saCas9 Nuclease Protein

K044 ABM 6.5 µg (50pmol) Volume: 50µL 55 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).;;The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT).

saCas9 Nuclease Protein

MBS4156505-00065mg MyBiosource 0.0065mg 210 EUR

saCas9 Nuclease Protein

MBS4156505-5x00065mg MyBiosource 5x0.0065mg 630 EUR

saCas9 Nuclease Protein

MBS4156520-003mg MyBiosource 0.03mg 275 EUR

saCas9 Nuclease Protein

MBS4156520-5x003mg MyBiosource 5x0.03mg 915 EUR

Cas9 Nuclease Lentivirus

K003 ABM 300 µl, 10^7 IU/ml, Titer: 10^7 IU/ml 315 EUR
Description: N/A

Cas9 Nuclease Adenovirus

K004 ABM 1.0 ml, 10^6 pfu/ml, Titer: 10^6 pfu/ml 315 EUR
Description: N/A

Cas9 Nuclease - 400µg - 1,600 ng/µl

3276-(HC) Intact Genomics 1/EA 678 EUR

Nuclease Mix 0.5mL - EACH

80650142 Scientific Laboratory Supplies EACH 191.7 EUR

Nuclease, Serratia marcescens

HY-131160 MedChemExpress 25 KU 270.57 EUR
Description: Serratia marcescens nuclease (EC 3.1.30.2) is a nonspecific nuclease. Serratia marcescens nuclease has broad utility due to its potent digestive activity toward both DNA and RNA[1][2].

Cas9 Nuclease - 80µg - 1,600 ng/µl

3273-(HC) Intact Genomics 1/EA 226.8 EUR

APEX2 (AP Endonuclease XTH2, APEX Nuclease-like 2, APEX Nuclease 2, Apurinic-apyrimidinic Endonuclease 2, AP Endonuclease 2, DNA-(apurinic or apyrimidinic site) Lyase 2, APE2, APEXL2, XTH2) (Azide free) (HRP)

MBS6463416-02mL MyBiosource 0.2mL 980 EUR

APEX2 (AP Endonuclease XTH2, APEX Nuclease-like 2, APEX Nuclease 2, Apurinic-apyrimidinic Endonuclease 2, AP Endonuclease 2, DNA-(apurinic or apyrimidinic site) Lyase 2, APE2, APEXL2, XTH2) (Azide free) (HRP)

MBS6463416-5x02mL MyBiosource 5x0.2mL 4250 EUR

Nuclease, Staphylococcus aureus

AS21-4632 Agrisera AB 10 mg 181 EUR

Nuclease P1 Antibody (HRP)

20-abx300889 Abbexa
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Nuclease P1 Antibody (HRP)

abx300889-100g Abbexa 100 µg 362.5 EUR

Nuclease P1 Antibody (HRP)

abx300889-20g Abbexa 20 µg 162.5 EUR

Nuclease P1 Antibody (HRP)

abx300889-50g Abbexa 50 µg 250 EUR

Rabbit polyclonal antibody to Apoptosis-enhancing nuclease (apoptosis enhancing nuclease)

TA308608 Origene Technologies GmbH 100 µl Ask for price

Nuclease P1 Antibody (FITC)

20-abx300890 Abbexa
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  • 100 ug
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  • 200 ug
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Cas9 Nuclease NLS Protein

K030 ABM 8.0 µg (50pmol) Volume: 50µL 40 EUR
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).;;The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants. Cas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.

Nuclease P1 Antibody (FITC)

abx300890-100g Abbexa 100 µg 362.5 EUR

Nuclease P1 Antibody (FITC)

abx300890-20g Abbexa 20 µg 162.5 EUR

Nuclease P1 Antibody (FITC)

abx300890-50g Abbexa 50 µg 250 EUR

Cas9 Nuclease NLS Protein

MBS4156498-0008mg MyBiosource 0.008mg 200 EUR

Cas9 Nuclease NLS Protein

MBS4156498-5x0008mg MyBiosource 5x0.008mg 595 EUR

Cas9 Nuclease NLS Protein

MBS4156513-004mg MyBiosource 0.04mg 230 EUR

Cas9 Nuclease NLS Protein

MBS4156513-5x004mg MyBiosource 5x0.04mg 710 EUR

Cas9 Nuclease NLS Protein

MBS4160529-004mg MyBiosource 0.04mg 195 EUR

Cas9 Nuclease NLS Protein

MBS4160529-5x004mg MyBiosource 5x0.04mg 640 EUR

Cas9 Nuclease NLS Protein

MBS4160530-02mg MyBiosource 0.2mg 330 EUR

Cas9 Nuclease NLS Protein

MBS4160530-5x02mg MyBiosource 5x0.2mg 1245 EUR

HARB1, NT (HARBI1, C11orf77, Putative nuclease HARBI1, Harbinger transposase-derived nuclease)

MBS6011663-02mL MyBiosource 0.2(mL 695 EUR

HARB1, NT (HARBI1, C11orf77, Putative nuclease HARBI1, Harbinger transposase-derived nuclease)

MBS6011663-5x02mL MyBiosource 5x0.2mL 2975 EUR

Nuclease Decontamination Solution

43094006-1 Glycomatrix 250 mL 43.46 EUR

Nuclease P1 Antibody (Biotin)

20-abx300891 Abbexa
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fnCpf1 Nuclease NLS Protein

K087 ABM 38 μg (250 pmol) Volume: 25µL 75 EUR
Description: Using Cpf1 (a.k.a. Cas12a) in your CRISPR experiment offers several advantages over other CRISPR-associated nucleases.;
  • Due to the T-rich PAM sequence (TTTN), Cpf1 enables editing in regions unable to be targeted by Cas9.
  • Cpf1 can be used with a shorter guide RNA (called crRNA) than Cas9.
  • Cpf1 creates a staggered cut in dsDNA instead of a blunt cut.
  • Cpf1 cuts distal to the PAM sequence, which may allow for multiple rounds of cleavage.
    • fnCpf1 is from the bacteria Francisella novicida. This protein contains a SV40 T antigen nuclear localization signal (NLS) on the N-terminus of the protein. If the cut caused by fnCpf1 is repaired by non-homologous end joining (NHEJ), an indel may be formed that disrupts the open reading frame of the targeted gene, leading to gene knockout. Alternatively, by supplying a repair template, a sequence can be knocked in at the cleavage site via homology directed repair (HDR).

Nuclease P1 Antibody (Biotin)

abx300891-100g Abbexa 100 µg 362.5 EUR

Nuclease P1 Antibody (Biotin)

abx300891-20g Abbexa 20 µg 162.5 EUR

Nuclease P1 Antibody (Biotin)

abx300891-50g Abbexa 50 µg 250 EUR

fnCpf1 Nuclease NLS Protein

MBS4156600-0035mg MyBiosource 0.035mg 225 EUR

fnCpf1 Nuclease NLS Protein

MBS4156600-5x0035mg MyBiosource 5x0.035mg 700 EUR

asCpf1 Nuclease NLS Protein

MBS4156601-0035mg MyBiosource 0.035mg 225 EUR

asCpf1 Nuclease NLS Protein

MBS4156601-5x0035mg MyBiosource 5x0.035mg 700 EUR

fnCpf1 Nuclease NLS Protein

MBS4156602-035mg MyBiosource 0.35mg 410 EUR

fnCpf1 Nuclease NLS Protein

MBS4156602-5x035mg MyBiosource 5x0.35mg 1525 EUR

asCpf1 Nuclease NLS Protein

MBS4156603-035mg MyBiosource 0.35mg 410 EUR

asCpf1 Nuclease NLS Protein

MBS4156603-5x035mg MyBiosource 5x0.35mg 1525 EUR

saCas9 Nuclease NLS Protein

MBS4160527-00065mg MyBiosource 0.0065mg 210 EUR

saCas9 Nuclease NLS Protein

MBS4160527-5x00065mg MyBiosource 5x0.0065mg 630 EUR

saCas9 Nuclease NLS Protein

MBS4160531-003mg MyBiosource 0.03mg 265 EUR

saCas9 Nuclease NLS Protein

MBS4160531-5x003mg MyBiosource 5x0.03mg 935 EUR

saCas9 Nuclease NLS Protein

MBS4160532-015mg MyBiosource 0.15mg 550 EUR

saCas9 Nuclease NLS Protein

MBS4160532-5x015mg MyBiosource 5x0.15mg 2220 EUR

saCas9 Nuclease NLS Protein

MBS4160533-003mg MyBiosource 0.03mg 275 EUR

saCas9 Nuclease NLS Protein

MBS4160533-5x003mg MyBiosource 5x0.03mg 915 EUR

Universal Nuclease ELISA Kit

RK04660 Abclonal 96T 280 EUR

Recombinant Human APEX nuclease-1

7-02476 CHI Scientific 2µg Ask for price

Recombinant Human APEX nuclease-1

7-02477 CHI Scientific 10µg Ask for price

Recombinant Human APEX nuclease-1

7-02478 CHI Scientific 1mg Ask for price

APEX Nuclease-1 Human Recombinant

rAP-1339 Angio Proteomie Inquiry Ask for price

Recombinant Human APEX nuclease-1

MBS144352-0002mg MyBiosource 0.002mg 240 EUR

Recombinant Human APEX nuclease-1

MBS144352-001mg MyBiosource 0.01mg 310 EUR
The presence of HpaB- and HrpF-independent transport routes means that the supply of AvrBs3 is initiated throughout early phases of the an infection course of, presumably earlier than the activation of HpaB or the insertion of the translocon into the plant plasma membrane.

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