Inducible modulation is usually required for exact investigations and manipulations of dynamic organic processes. Transcription activator-like effectors (TALEs) present a robust device for focused gene modifying and transcriptional programming. We designed a sequence of chemical inducible methods by coupling TALEs with a mutated human estrogen receptor (ERT2), which renders them 4-hydroxyl-tamoxifen (4-OHT) inducible for entry of the genome.
Chemical inducible genome modifying was achieved by way of fusing two tandem ERT2 domains to personalised transcription activator-like effector nuclease (TALEN), which we termed “Hybrid Inducible Technology” (HIT-TALEN). Those for transcription activation had been vigorously optimized utilizing a number of assemble designs. Most environment friendly drug induction for endogenous gene activation was achieved with minimal background exercise utilizing an optimized inducible TALE based mostly SunTag system (HIT-TALE-SunTag).
The HIT-SunTag system is fast, tunable, selective to 4-OHT over an endogenous ligand, and reversible in drug induced transcriptional activation. Versatile methods developed on this research may be simply utilized for modifying and transcriptional programming of doubtless any genomic loci in a good and efficient chemical inducible trend.
Xanthomonas TAL effectors hijack host basal transcription issue IIA α and γ subunits for invasion.
The Xanthomonas genus consists of Gram-negative plant-pathogenic micro organism, which infect a broad vary of crops and wild plant species, trigger signs with leaf blights, streaks, spots, stripes, necrosis, wilt, cankers and gummosis on leaves, stems and fruits in all kinds of crops by way of injecting their effector proteins into the host cell throughout an infection. Among these virulent effectors, transcription activator-like effectors (TALEs) work together with the γ subunit of host transcription issue IIA (TFIIAγ) to activate the transcription of host illness susceptibility genes.
Functional TFIIA is a ternary advanced comprising α, β and γ subunits. However, whether or not TALEs recruit TFIIAα, TFIIAβ, or each stays unknown. The underlying molecular mechanisms by which TALEs mediate host susceptibility gene activation require full elucidation. Here, we present that TALEs work together with the α+γ binary subcomplex however not the α+β+γ ternary advanced of rice TFIIA (holo-OsTFIIA). The transcription issue binding (TFB) areas of TALEs, that are extremely conserved in Xanthomonas species, have a dominant function in these interactions.
Furthermore, the interplay between TALEs and the α+γ advanced displays sturdy DNA binding exercise in vitro. These outcomes collectively reveal that TALE-carrying pathogens hijack the host basal transcription components TFIIAα and TFIIAγ, however not TFIIAβ, to reinforce host susceptibility throughout pathogen an infection. The uncovered mechanism widens new insights on host-microbe interplay and present an relevant technique to breed high-resistance crop varieties.
A Conserved Basal Transcription Factor Is Required for the Function of Diverse TAL Effectors in Multiple Plant Hosts.
Many Xanthomonas micro organism use transcription activator-like effector (TALE) proteins to activate plant illness susceptibility (S) genes, and this activation contributes to illness. We not too long ago reported that rice basal transcription issue IIA gamma subunit, OsTFIIAγ5, is hijacked by TALE-carrying Xanthomonas oryzae infecting the crops.
However, whether or not TFIIAγs are additionally concerned in TALE-carrying Xanthomonas-caused illnesses in different crops is unknown. Here, molecular and genetic approaches had been used to research the function of TFIIAγs in different crops. We discovered that TFIIAγs are additionally utilized by TALE-carrying Xanthomonas to trigger illness in different crops.
The TALEs of Xanthomonas citri pv. citri (Xcc) inflicting canker in citrus and Xanthomonas campestris pv. vesicatoria (Xcv) inflicting bacterial spot in pepper and tomato interacted with corresponding host TFIIAγs as in rice. Transcriptionally suppressing TFIIAγ led to resistance to Xcc in citrus and Xcv in pepper and tomato. The 39th residue of OsTFIIAγ5 and citrus CsTFIIAγ is significant for TALE-dependent induction of plant S genes.
As mutated OsTFIIAγ5V 39E, CsTFIIAγV 39E, pepper CaTFIIAγV 39E, and tomato SlTFIIAγV 39E additionally didn’t work together with TALEs to forestall illness. These outcomes counsel that TALE-carrying micro organism share a standard mechanism for infecting crops. Using TFIIAγV 39E-type mutation could possibly be a normal technique for bettering resistance to TALE-carrying pathogens in crops.
The TAL Effector AvrBs3 from Xanthomonas campestris pv. vesicatoria Contains Multiple Export Signals and Can Enter Plant Cells within the Absence of the Type III Secretion Translocon.
Pathogenicity of the Gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria depends upon a kind III secretion (T3S) system which translocates effector proteins into plant cells. Effector protein supply is managed by the T3S chaperone HpaB, which presumably escorts effector proteins to the secretion equipment.
One intensively studied effector is the transcription activator-like (TAL) effector AvrBs3, which binds to promoter sequences of plant goal genes and prompts plant gene expression. It was beforehand reported that kind III-dependent supply of AvrBs3 depends upon the N-terminal protein area. The indicators that management T3S and translocation of AvrBs3, nonetheless, haven’t but been characterised. In the current research, we present that T3S and translocation of AvrBs3 depend upon the N-terminal 10 and 50 amino acids, respectively.
Furthermore, we offer experimental proof that further indicators within the N-terminal 30 amino acids and the area between amino acids 64 and 152 promote translocation of AvrBs3 within the absence of HpaB. Unexpectedly, in vivo translocation assays revealed that AvrBs3 is delivered into plant cells even within the absence of HrpF, which is the anticipated channel-forming part of the T3S translocon within the plant plasma membrane.
Water, Sterile, Nuclease Free |
|||
| MBS635323-500mL | MyBiosource | 500mL | 135 EUR |
Water, Sterile, Nuclease Free |
|||
| MBS635323-5x3L | MyBiosource | 5x3L | 1090 EUR |
Bovine Albumin Nuclease Free |
|||
| IBOALBNF100MG | Innovative research | each | 136 EUR |
|
Description: Bovine Albumin Nuclease Free |
|||
Bovine Albumin Nuclease Free |
|||
| IBOALBNF500MG | Innovative research | each | 347 EUR |
|
Description: Bovine Albumin Nuclease Free |
|||
Bovine Albumin Nuclease Free |
|||
| MBS136206-100mg | MyBiosource | 100mg | 235 EUR |
Bovine Albumin Nuclease Free |
|||
| MBS136206-500mg | MyBiosource | 500mg | 450 EUR |
Bovine Albumin Nuclease Free |
|||
| MBS136206-5x500mg | MyBiosource | 5x500mg | 1850 EUR |
ClearBand Nuclease Free Ultra-Pure Water |
|||
| DW003L | Ecotech Biotechnology | 30 ml | 5.5 EUR |
|
Description: ClearBand Nuclease Free Ultra-Pure Water is prepared under stringent conditions and is suitable for all applications in a molecular biology laboratory. ClearBandNuclease Free Ultra-Pure Water goes through, deionization, reverse osmosis, UV-treatment, 0.2μm filtration, and double autoclave to ensure sterility, being free of nucleases and to completely eliminate the byproducts of DEPC. |
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ClearBand Nuclease Free Ultra-Pure Water |
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| DW010L | Ecotech Biotechnology | 100 ml | 7.7 EUR |
|
Description: ClearBand Nuclease Free Ultra-Pure Water is prepared under stringent conditions and is suitable for all applications in a molecular biology laboratory. ClearBandNuclease Free Ultra-Pure Water goes through, deionization, reverse osmosis, UV-treatment, 0.2μm filtration, and double autoclave to ensure sterility, being free of nucleases and to completely eliminate the byproducts of DEPC. |
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ClearBand Nuclease Free Ultra-Pure Water |
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| DW05L | Ecotech Biotechnology | 500 ml | 12.1 EUR |
|
Description: ClearBand Nuclease Free Ultra-Pure Water is prepared under stringent conditions and is suitable for all applications in a molecular biology laboratory. ClearBandNuclease Free Ultra-Pure Water goes through, deionization, reverse osmosis, UV-treatment, 0.2μm filtration, and double autoclave to ensure sterility, being free of nucleases and to completely eliminate the byproducts of DEPC. |
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ClearBand Nuclease Free Ultra-Pure Water |
|||
| DW1L | Ecotech Biotechnology | 1 L | 17.6 EUR |
|
Description: ClearBand Nuclease Free Ultra-Pure Water is prepared under stringent conditions and is suitable for all applications in a molecular biology laboratory. ClearBandNuclease Free Ultra-Pure Water goes through, deionization, reverse osmosis, UV-treatment, 0.2μm filtration, and double autoclave to ensure sterility, being free of nucleases and to completely eliminate the byproducts of DEPC. |
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Nuclease Free, Water, Ultra Pure Grade, 500ml |
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| PB0100-500ml | Vivantis | each | Ask for price |
Water, Sterile, Nuclease & Endotoxin Free |
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| MBS635296-100mL | MyBiosource | 100mL | 150 EUR |
Water, Sterile, Nuclease & Endotoxin Free |
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| MBS635296-1L | MyBiosource | 1L | 380 EUR |
Water, Sterile, Nuclease & Endotoxin Free |
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| MBS635296-250mL | MyBiosource | 250mL | 190 EUR |
Water, Sterile, Nuclease & Endotoxin Free |
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| MBS635296-500mL | MyBiosource | 500mL | 255 EUR |
Water, Sterile, Nuclease & Endotoxin Free |
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| MBS635296-50mL | MyBiosource | 50mL | 130 EUR |
Water, Ultrapure, Nuclease Free, 0.1um Filtered, DNase, RNase and Protease Free |
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| W0807-050 | GenDepot | 500 ml | 40 EUR |
Water, Ultrapure, Nuclease Free, 0.1um Filtered, DNase, RNase and Protease Free |
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| W0807-100 | GenDepot | 1L | 50 EUR |
Nuclease-free H2O |
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| RK20102 | Abclonal | 25mL | 21.5 EUR |
Water, nuclease-free |
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| 96370 | Sisco Laboratories | 5 x1 ml | 2.48 EUR |
|
Description: Part E |
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Water, nuclease-free |
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| 96370-1 | Sisco Laboratories | 100 ml | 4.7 EUR |
|
Description: Part E |
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Water, nuclease-free |
|||
| 96370-2 | Sisco Laboratories | 500 ml | 14.63 EUR |
|
Description: Part E |
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Nuclease-free Water |
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| abx098144-100l | Abbexa | 100 µl | 212.5 EUR |
Nuclease-free Water |
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| abx098144-1ml | Abbexa | 1 ml | Ask for price |
Nuclease-free Water |
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| abx098144-200l | Abbexa | 200 µl | Ask for price |
Nuclease P1 rabbit polyclonal antibody, Azide Free |
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| AP21351AF-N | Origene Technologies GmbH | 10 mg | Ask for price |
HARB1, NT (HARBI1, C11orf77, Putative nuclease HARBI1, Harbinger transposase-derived nuclease) (Azide free) (HRP) |
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| MBS6305431-02mL | MyBiosource | 0.2mL | 980 EUR |
HARB1, NT (HARBI1, C11orf77, Putative nuclease HARBI1, Harbinger transposase-derived nuclease) (Azide free) (HRP) |
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| MBS6305431-5x02mL | MyBiosource | 5x0.2mL | 4250 EUR |
Nuclease-free water, not DEPC treated |
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| E0212-01 | EURx | 100ml | 21.8 EUR |
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Description: For DNA targeted reactions such as PCR, ligation and restriction digestions |
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Nuclease-free water, not DEPC treated |
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| E0212-02 | EURx | 1l | 57.77 EUR |
|
Description: For DNA targeted reactions such as PCR, ligation and restriction digestions |
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BioMag® Nuclease-Free Streptavidin Particles |
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| 8MB4804-1 | Polysciences Europe GmbH | 100ml | 3034 EUR |
BioMag® Nuclease-Free Streptavidin Particles |
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| 8MB4804-10 | Polysciences Europe GmbH | 10ml | 326 EUR |
BioMag® Nuclease-Free Streptavidin Particles |
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| 8MB4804-25 | Polysciences Europe GmbH | 25ml | 892 EUR |
BenzNuclease DNA and RNA Nuclease (Ultra Pure, Protease Free) Recombinant Protein |
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| 11-552 | ProSci | 50KU | 481.2 EUR |
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Description: BenzNuclease DNA and RNA Nuclease (Ultra Pure, Protease Free) Recombinant Protein |
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MTMRF, NT (FAN1, KIAA1018, MTMR15, Fanconi-associated nuclease 1, FANCD2/FANCI-associated nuclease 1, Myotubularin-related protein 15) (Azide free) (HRP) |
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| MBS6322839-02mL | MyBiosource | 0.2mL | 980 EUR |
MTMRF, NT (FAN1, KIAA1018, MTMR15, Fanconi-associated nuclease 1, FANCD2/FANCI-associated nuclease 1, Myotubularin-related protein 15) (Azide free) (HRP) |
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| MBS6322839-5x02mL | MyBiosource | 5x0.2mL | 4250 EUR |
Bacteria-Derived BenzNuclease DNA and RNA Nuclease (Ultra Pure, Protease Free) Recombinant Protein Tag Free Lyophilized |
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| IBCTBZNCDNARNANUPPROFRTFLY50KU | Innovative research | each | 535 EUR |
|
Description: Bacteria-Derived BenzNuclease DNA and RNA Nuclease (Ultra Pure, Protease Free) Recombinant Protein Tag Free Lyophilized |
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Bacteria-Derived BenzNuclease DNA and RNA Nuclease (Ultra Pure, Protease Free) Recombinant Protein Tag Free Lyophilized |
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| MBS8430529-INQUIRE | MyBiosource | INQUIRE | Ask for price |
S1 Nuclease |
|||
| E1335-01 | EURx | 10000U | 43.6 EUR |
|
Description: Non-specific endonuclease, active primarily on single-stranded DNA and RNA |
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S1 Nuclease |
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| E1335-02 | EURx | 50000U | 168.95 EUR |
|
Description: Non-specific endonuclease, active primarily on single-stranded DNA and RNA |
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Nuclease AWAY |
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| A6257-100ML | Biomatik Corporation | 100ML | 22 EUR |
|
Description: Biotechnology |
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Nuclease AWAY |
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| A6257-500ML | Biomatik Corporation | 500ML | 88 EUR |
|
Description: Biotechnology |
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Cas9 Nuclease |
|||
| 310 | GeneOn | 2x40µg (2x250 pmol) | 316.8 EUR |
Cas9 Nuclease |
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| 312HC | GeneOn | 80 µg (500 pmol) | 268.8 EUR |
Cas9 Nuclease |
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| 314HC | GeneOn | 400 µg (2500 pmol) | 894 EUR |
Cas9 Nuclease |
|||
| 3273 | Intact Genomics | 80µg(1600ng/µl),500pmol | 107.2 EUR |
Cas9 Nuclease |
|||
| 3276 | Intact Genomics | 400µg(1600ng/µl),2500pmol | 396 EUR |
OMNI Nuclease |
|||
| E1120-01 | EURx | 20000U | 35.97 EUR |
|
Description: Nonspecific nuclease that completely degrades all forms of DNA and RNA |
|||
OMNI Nuclease |
|||
| E1120-02 | EURx | 100000U | 162.41 EUR |
|
Description: Nonspecific nuclease that completely degrades all forms of DNA and RNA |
|||
Cas9 Nuclease |
|||
| EN301-01 | Vazyme | 50 pmol | 146.4 EUR |
Cas9 Nuclease |
|||
| EN301-02 | Vazyme | 250 pmol | 184.8 EUR |
Ultra Nuclease |
|||
| HBP000106 | HZymes Biotechnology | 20μL | 14.73 EUR |
Ultra Nuclease |
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| HBP000107 | HZymes Biotechnology | 200μL | 116.82 EUR |
Ultra Nuclease |
|||
| HBP000108 | HZymes Biotechnology | 2mL | 965.04 EUR |
Ultra Nuclease |
|||
| HBP000109 | HZymes Biotechnology | 20mL | 8634.56 EUR |
Turbo Nuclease |
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| EN-180L | Jena Bioscience GmbH | 5 x 10000units | 384.5 EUR |
|
Description: Serratia marcescens, recombinant, E. coli |
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Turbo Nuclease |
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| EN-180S | Jena Bioscience GmbH | 10000units | 96.2 EUR |
|
Description: Serratia marcescens, recombinant, E. coli |
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Cas12a Nuclease |
|||
| 3373 | Intact Genomics | 80µg(1600ng/µl) | 123.2 EUR |
Cas12a Nuclease |
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| 3376 | Intact Genomics | 400µg(1600ng/µl) | 420 EUR |
Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase, NP1) |
|||
| MBS653730-100Units | MyBiosource | 100Units | 320 EUR |
Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase, NP1) |
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| MBS653730-250Units | MyBiosource | 250Units | 635 EUR |
Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase, NP1) |
|||
| MBS653730-5x250Units | MyBiosource | 5x250Units | 2710 EUR |
Microccal Nuclease |
|||
| TBS6020 | Tribioscience | 10KU | 89 EUR |
Benzonase Nuclease |
|||
| MBS157420-100KUnits | MyBiosource | 100KUnits | 320 EUR |
Benzonase Nuclease |
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| MBS157420-25KUnits | MyBiosource | 25KUnits | 210 EUR |
Benzonase Nuclease |
|||
| MBS157420-50KUnits | MyBiosource | 50KUnits | 245 EUR |
Benzonase Nuclease |
|||
| MBS157420-5x100KUnits | MyBiosource | 5x100KUnits | 1115 EUR |
APEX Nuclease I |
|||
| MBS4380592-002mgWithBSAAzideat02mgmL | MyBiosource | 0.02mg(WithBSA&Azideat0.2mg/mL) | 230 EUR |
APEX Nuclease I |
|||
| MBS4380592-01mgWithBSAAzideat02mgmL | MyBiosource | 0.1mg(WithBSA&Azideat0.2mg/mL) | 405 EUR |
APEX Nuclease I |
|||
| MBS4380592-01mgWithoutBSAAzideat1mgmL | MyBiosource | 0.1mg(WithoutBSA&Azideat1mg/mL) | 405 EUR |
APEX Nuclease I |
|||
| MBS4380592-5x01mgWithBSAAzideat02mgmL | MyBiosource | 5x0.1mg(WithBSA&Azideat0.2mg/mL) | 1725 EUR |
APEX Nuclease I |
|||
| MBS4380592-5x01mgWithoutBSAAzideat1mgmL | MyBiosource | 5x0.1mg(WithoutBSA&Azideat1mg/mL) | 1725 EUR |
GENIUS™Nuclease |
|||
| BEE-N3116-10KU | ACROBIOSYSTEMS | 10KU | 98.1 EUR |
|
Description: GENIUS™Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-5 bases in length. |
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GENIUS™Nuclease |
|||
| BEE-N3116-500KU | ACROBIOSYSTEMS | 500KU | 784.8 EUR |
|
Description: GENIUS™Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-5 bases in length. |
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GENIUS™Nuclease |
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| BEE-N3116-50KU | ACROBIOSYSTEMS | 50KU | 196.2 EUR |
|
Description: GENIUS™Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-5 bases in length. |
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Nuclease P1 Antibody |
|||
| 20-abx300888 | Abbexa |
|
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Nuclease P1 Antibody |
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| 1-CSB-PA328459LA01PEU | Cusabio |
|
|
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Description: A polyclonal antibody against Nuclease P1. Recognizes Nuclease P1 from Penicillium citrinum. This antibody is Unconjugated. Tested in the following application: ELISA |
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Nuclease P1 Antibody |
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| abx300888-100g | Abbexa | 100 µg | 362.5 EUR |
Nuclease P1 Antibody |
|||
| abx300888-20g | Abbexa | 20 µg | 162.5 EUR |
Nuclease P1 Antibody |
|||
| abx300888-50g | Abbexa | 50 µg | 250 EUR |
Nuclease P1 Antibody |
|||
| MBS7003563-005mg | MyBiosource | 0.05mg | 190 EUR |
Nuclease P1 Antibody |
|||
| MBS7003563-01mg | MyBiosource | 0.1mg | 270 EUR |
Nuclease P1 Antibody |
|||
| MBS7003563-5x01mg | MyBiosource | 5x0.1mg | 1205 EUR |
Benzonase Nuclease, 99% |
|||
| enz-1112 | ProSpec Tany | 5000U | 90 EUR |
|
Description: Recombinant Serratia Marcescens Benzonase Nuclease, 99% |
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Benzonase Nuclease, 90% |
|||
| enz-1150 | ProSpec Tany | 5000U | 70 EUR |
|
Description: Recombinant Serratia Marcescens Benzonase Nuclease, 90% |
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Cas9 Nuclease Protein |
|||
| K008 | ABM | 8.0 µg (50pmol) Volume: 50µL | 40 EUR |
|
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).;;The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants. |
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Cas9 Nuclease Protein |
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| MBS4156497-0008mg | MyBiosource | 0.008mg | 200 EUR |
Cas9 Nuclease Protein |
|||
| MBS4156497-5x0008mg | MyBiosource | 5x0.008mg | 595 EUR |
Cas9 Nuclease Protein |
|||
| MBS4156512-004mg | MyBiosource | 0.04mg | 230 EUR |
Cas9 Nuclease Protein |
|||
| MBS4156512-5x004mg | MyBiosource | 5x0.04mg | 710 EUR |
saCas9 Nuclease Protein |
|||
| K044 | ABM | 6.5 µg (50pmol) Volume: 50µL | 55 EUR |
|
Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).;;The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT). |
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saCas9 Nuclease Protein |
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| MBS4156505-00065mg | MyBiosource | 0.0065mg | 210 EUR |
saCas9 Nuclease Protein |
|||
| MBS4156505-5x00065mg | MyBiosource | 5x0.0065mg | 630 EUR |
saCas9 Nuclease Protein |
|||
| MBS4156520-003mg | MyBiosource | 0.03mg | 275 EUR |
saCas9 Nuclease Protein |
|||
| MBS4156520-5x003mg | MyBiosource | 5x0.03mg | 915 EUR |
Cas9 Nuclease Lentivirus |
|||
| K003 | ABM | 300 µl, 10^7 IU/ml, Titer: 10^7 IU/ml | 315 EUR |
|
Description: N/A |
|||
Cas9 Nuclease Adenovirus |
|||
| K004 | ABM | 1.0 ml, 10^6 pfu/ml, Titer: 10^6 pfu/ml | 315 EUR |
|
Description: N/A |
|||
Cas9 Nuclease - 400µg - 1,600 ng/µl |
|||
| 3276-(HC) | Intact Genomics | 1/EA | 678 EUR |
Nuclease Mix 0.5mL - EACH |
|||
| 80650142 | Scientific Laboratory Supplies | EACH | 191.7 EUR |
Nuclease, Serratia marcescens |
|||
| HY-131160 | MedChemExpress | 25 KU | 270.57 EUR |
|
Description: Serratia marcescens nuclease (EC 3.1.30.2) is a nonspecific nuclease. Serratia marcescens nuclease has broad utility due to its potent digestive activity toward both DNA and RNA[1][2]. |
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Cas9 Nuclease - 80µg - 1,600 ng/µl |
|||
| 3273-(HC) | Intact Genomics | 1/EA | 226.8 EUR |
APEX2 (AP Endonuclease XTH2, APEX Nuclease-like 2, APEX Nuclease 2, Apurinic-apyrimidinic Endonuclease 2, AP Endonuclease 2, DNA-(apurinic or apyrimidinic site) Lyase 2, APE2, APEXL2, XTH2) (Azide free) (HRP) |
|||
| MBS6463416-02mL | MyBiosource | 0.2mL | 980 EUR |
APEX2 (AP Endonuclease XTH2, APEX Nuclease-like 2, APEX Nuclease 2, Apurinic-apyrimidinic Endonuclease 2, AP Endonuclease 2, DNA-(apurinic or apyrimidinic site) Lyase 2, APE2, APEXL2, XTH2) (Azide free) (HRP) |
|||
| MBS6463416-5x02mL | MyBiosource | 5x0.2mL | 4250 EUR |
Nuclease, Staphylococcus aureus |
|||
| AS21-4632 | Agrisera AB | 10 mg | 181 EUR |
Nuclease P1 Antibody (HRP) |
|||
| 20-abx300889 | Abbexa |
|
|
Nuclease P1 Antibody (HRP) |
|||
| abx300889-100g | Abbexa | 100 µg | 362.5 EUR |
Nuclease P1 Antibody (HRP) |
|||
| abx300889-20g | Abbexa | 20 µg | 162.5 EUR |
Nuclease P1 Antibody (HRP) |
|||
| abx300889-50g | Abbexa | 50 µg | 250 EUR |
Rabbit polyclonal antibody to Apoptosis-enhancing nuclease (apoptosis enhancing nuclease) |
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| TA308608 | Origene Technologies GmbH | 100 µl | Ask for price |
Nuclease P1 Antibody (FITC) |
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| 20-abx300890 | Abbexa |
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Cas9 Nuclease NLS Protein |
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| K030 | ABM | 8.0 µg (50pmol) Volume: 50µL | 40 EUR |
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Description: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).;;The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants. Cas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein. |
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Nuclease P1 Antibody (FITC) |
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| abx300890-100g | Abbexa | 100 µg | 362.5 EUR |
Nuclease P1 Antibody (FITC) |
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| abx300890-20g | Abbexa | 20 µg | 162.5 EUR |
Nuclease P1 Antibody (FITC) |
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| abx300890-50g | Abbexa | 50 µg | 250 EUR |
Cas9 Nuclease NLS Protein |
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| MBS4156498-0008mg | MyBiosource | 0.008mg | 200 EUR |
Cas9 Nuclease NLS Protein |
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| MBS4156498-5x0008mg | MyBiosource | 5x0.008mg | 595 EUR |
Cas9 Nuclease NLS Protein |
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| MBS4156513-004mg | MyBiosource | 0.04mg | 230 EUR |
Cas9 Nuclease NLS Protein |
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| MBS4156513-5x004mg | MyBiosource | 5x0.04mg | 710 EUR |
Cas9 Nuclease NLS Protein |
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| MBS4160529-004mg | MyBiosource | 0.04mg | 195 EUR |
Cas9 Nuclease NLS Protein |
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| MBS4160529-5x004mg | MyBiosource | 5x0.04mg | 640 EUR |
Cas9 Nuclease NLS Protein |
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| MBS4160530-02mg | MyBiosource | 0.2mg | 330 EUR |
Cas9 Nuclease NLS Protein |
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| MBS4160530-5x02mg | MyBiosource | 5x0.2mg | 1245 EUR |
HARB1, NT (HARBI1, C11orf77, Putative nuclease HARBI1, Harbinger transposase-derived nuclease) |
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| MBS6011663-02mL | MyBiosource | 0.2(mL | 695 EUR |
HARB1, NT (HARBI1, C11orf77, Putative nuclease HARBI1, Harbinger transposase-derived nuclease) |
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| MBS6011663-5x02mL | MyBiosource | 5x0.2mL | 2975 EUR |
Nuclease Decontamination Solution |
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| 43094006-1 | Glycomatrix | 250 mL | 43.46 EUR |
Nuclease P1 Antibody (Biotin) |
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| 20-abx300891 | Abbexa |
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fnCpf1 Nuclease NLS Protein |
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| K087 | ABM | 38 μg (250 pmol) Volume: 25µL | 75 EUR |
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Description: Using Cpf1 (a.k.a. Cas12a) in your CRISPR experiment offers several advantages over other CRISPR-associated nucleases.;
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Nuclease P1 Antibody (Biotin) |
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| abx300891-100g | Abbexa | 100 µg | 362.5 EUR |
Nuclease P1 Antibody (Biotin) |
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| abx300891-20g | Abbexa | 20 µg | 162.5 EUR |
Nuclease P1 Antibody (Biotin) |
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| abx300891-50g | Abbexa | 50 µg | 250 EUR |
fnCpf1 Nuclease NLS Protein |
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| MBS4156600-0035mg | MyBiosource | 0.035mg | 225 EUR |
fnCpf1 Nuclease NLS Protein |
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| MBS4156600-5x0035mg | MyBiosource | 5x0.035mg | 700 EUR |
asCpf1 Nuclease NLS Protein |
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| MBS4156601-0035mg | MyBiosource | 0.035mg | 225 EUR |
asCpf1 Nuclease NLS Protein |
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| MBS4156601-5x0035mg | MyBiosource | 5x0.035mg | 700 EUR |
fnCpf1 Nuclease NLS Protein |
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| MBS4156602-035mg | MyBiosource | 0.35mg | 410 EUR |
fnCpf1 Nuclease NLS Protein |
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| MBS4156602-5x035mg | MyBiosource | 5x0.35mg | 1525 EUR |
asCpf1 Nuclease NLS Protein |
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| MBS4156603-035mg | MyBiosource | 0.35mg | 410 EUR |
asCpf1 Nuclease NLS Protein |
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| MBS4156603-5x035mg | MyBiosource | 5x0.35mg | 1525 EUR |
saCas9 Nuclease NLS Protein |
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| MBS4160527-00065mg | MyBiosource | 0.0065mg | 210 EUR |
saCas9 Nuclease NLS Protein |
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| MBS4160527-5x00065mg | MyBiosource | 5x0.0065mg | 630 EUR |
saCas9 Nuclease NLS Protein |
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| MBS4160531-003mg | MyBiosource | 0.03mg | 265 EUR |
saCas9 Nuclease NLS Protein |
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| MBS4160531-5x003mg | MyBiosource | 5x0.03mg | 935 EUR |
saCas9 Nuclease NLS Protein |
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| MBS4160532-015mg | MyBiosource | 0.15mg | 550 EUR |
saCas9 Nuclease NLS Protein |
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| MBS4160532-5x015mg | MyBiosource | 5x0.15mg | 2220 EUR |
saCas9 Nuclease NLS Protein |
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| MBS4160533-003mg | MyBiosource | 0.03mg | 275 EUR |
saCas9 Nuclease NLS Protein |
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| MBS4160533-5x003mg | MyBiosource | 5x0.03mg | 915 EUR |
Universal Nuclease ELISA Kit |
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| RK04660 | Abclonal | 96T | 280 EUR |
Recombinant Human APEX nuclease-1 |
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| 7-02476 | CHI Scientific | 2µg | Ask for price |
Recombinant Human APEX nuclease-1 |
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| 7-02477 | CHI Scientific | 10µg | Ask for price |
The presence of HpaB- and HrpF-independent transport routes means that the supply of AvrBs3 is initiated throughout early phases of the an infection course of, presumably earlier than the activation of HpaB or the insertion of the translocon into the plant plasma membrane.
