Monday, 28 November, 2022

Tal Effectors

Transcription activator-like effector nuclease

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  • Non-TAL Effectors From Xanthomonas oryzae pv. oryzae Suppress Peptidoglycan-Triggered MAPK Activation in Rice.
Non-TAL Effectors From Xanthomonas oryzae pv. oryzae Suppress Peptidoglycan-Triggered MAPK Activation in Rice.
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Non-TAL Effectors From Xanthomonas oryzae pv. oryzae Suppress Peptidoglycan-Triggered MAPK Activation in Rice.

Xanthomonas oryzae pv. oryzae, the causal pathogen of bacterial blight of rice, depends upon its sort III secretion system and related effector proteins to develop and colonize the vascular tissues of rice crops. The sort III effectors embrace a household of intently associated transcription activator-like (TAL) effectors and the remainder of various effectors, so-called non-TAL effectors. Our understanding of non-TAL effectors for pathogenesis in rice blight continues to be restricted.
Here we report a possible methodology to quickly detect the activation of mitogen-activated protein kinase pathway in rice mesophyll protoplasts by the Xoryzae pv. oryzae derived peptidoglycan and display screen for virulent effectors that may suppress the pathogen-associated molecular sample triggered immunity (PTI) response.
Amongst 17 non-TAL effectors transiently expressed in rice cells, we discovered that three effectors (XopZ, XopN, and XopV) have been capable of suppress the peptidoglycan-triggered MAPK activation. The triple mutant of the X. oryzae pv. oryzae pressure PXO99A missing XopZXopN, and XopV confirmed additively decreased virulence. Adding again both of genes restored the virulence of the triple mutant. Our outcomes exhibit the collective and redundant capability of protection suppression by non-TAL effectors in inflicting bacterial blight of rice.

 A Simple TAL Effector Assembly Reaction Using Isothermal Assembly.

Transcription activator-like effectors (TALEs) comprise programmable DNA-binding domains that may be fused to numerous effectors to control genetic sequences or transcriptional state. However, the development of plasmids encoding the modular DNA-binding area stays difficult as a consequence of their repetitive nature. Here, we describe strategies for a easy TALE meeting response (STAR) that makes use of a 68-part plasmid library to create TALEs binding to 17 bp goal sequences.
Manual manufacturing of many tens of TALEs may be achieved utilizing a easy eight h protocol, with full size sequence-verified plasmids obtainable inside just a few days. This easy story meeting response (STAR) offers a handy methodology for producing tens to tons of of TALENs or TALE-TFs with out the necessity for giant plasmid libraries or costly liquid dealing with.

Efficient enrichment cloning of TAL effector genes from Xanthomonas.

Many plant-pathogenic xanthomonads use a sort III secretion system to translocate Transcription Activator-Like (TAL) effectors into eukaryotic host cells the place they act as transcription components. Target genes are induced upon binding of a TAL effector to double-stranded DNA in a sequence-specific method. DNA binding is ruled by a extremely repetitive protein area, which consists of an array of almost equivalent repeats of ca. 102 base pairs.
Many species and pathovars of Xanthomonas, together with pathogens of rice, cereals, cassava, citrus and cotton, encode a number of TAL effectors in their genomes. Some of the TAL effectors have been proven to behave as key pathogenicity components, which induce the expression of susceptibility genes to the advantage of the pathogen. However, as a result of repetitive character and the presence of a number of gene copies, high-throughput cloning of TAL effector genes stays a problem.
Non-TAL Effectors From Xanthomonas oryzae pv. oryzae Suppress Peptidoglycan-Triggered MAPK Activation in Rice.
In order to isolate full TAL effector gene repertoires, we developed an enrichment cloning technique based mostly on •genome-informed in silico optimization of restriction digestions,•selective restriction digestion of genomic DNA, and•measurement fractionation of DNA fragments. Our fast, low-cost and highly effective methodology permits environment friendly cloning of TAL effector genes from xanthomonads, as demonstrated for 2 rice-pathogenic strains of Xanthomonas oryzae from Africa.

Ectopic expression of the TAL effector AvrXa7 in Xanthomonas citri subsp. citri hinders citrus canker symptom formation by modulating transcriptional profile of citrus genes.

Xanthomonas citri subsp. citri (Xcc) is the causal agent of citrus canker, a severe bacterial illness that impacts citrus bushes worldwide. The ectopic expression of TAL effector AvrXa7 in Xcc suppressed canker improvement. The Xcc pressure expressing avrXa7 induced a yellow symptom across the inoculation website. Transcriptome evaluation revealed 315 differentially expressed genes, which have been categorized into a number of practical teams.
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The extra fascinating genes have been these concerned in the biosynthesis of terpene and ethylene. In specific, the linoleate 13 S-lipoxygenase gene CsLOX2-1 was discovered to own the AvrXa7 binding sequence in the promoter area. The recognition of AvrXa7 to the CsLOX2-1 promoter was subsequently confirmed by yeast one-hybrid and electrophoretic mobility shift experiments. This demonstrated that the TALE effector AvrXa7 promotes CsLOX2-1 expression by immediately binding to the promoter sequence. Our findings contribute a worthwhile clue to figuring out the potential genes that can be utilized to stop citrus canker.

Two ancestral genes formed the Xanthomonas campestris TAL effector gene repertoire.

Xanthomonas transcription activator-like effectors (TALEs) are injected inside plant cells to advertise host susceptibility by enhancing transcription of host susceptibility genes. TALE-encoding (tal) genes have been considered absent from Brassicaceae-infecting Xanthomonas campestris (Xc) genomes based mostly on 4 reference genomic sequences. We found tal genes in 26 of 49 Xc strains remoted worldwide and used a mixture of single molecule actual time (SMRT) and tal amplicon sequencing to yield a near-complete description of the TALEs discovered in Xc (Xc TALome).
The 53 sequenced tal genes encode 21 distinct DNA binding domains that kind into seven main DNA binding specificities. In silico evaluation of the Brassica rapa promoterome recognized a repertoire of predicted TALE targets, 5 of which have been experimentally validated utilizing quantitative reverse transcription polymerase chain response.

Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated

S0014 Affbiotech 200ul 304 EUR

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647

A6-686-C100 ExBio 0.1 mg 269 EUR

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647

A6-712-C100 ExBio 0.1 mg 269 EUR

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647

A6-730-C100 ExBio 0.1 mg 269 EUR

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647

A6-737-C100 ExBio 0.1 mg 269 EUR

Endoglin/CD105 Alexa Fluor

FC15024 Neuromics 100 Tests 448 EUR

SAM FCM (Alexa Fluor 488)

abx098904-60tests Abbexa 60 tests 1358 EUR

EGFR antibody (Alexa Fluor 488)

61R-E109BAF Fitzgerald 125 ug 706 EUR
Description: Mouse monoclonal EGFR antibody (Alexa Fluor 488)

Anti-CD40 antibody (Alexa-fluor 488)

STJ170000 St John's Laboratory 100 µg 393 EUR
Description: CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40L (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40, predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin

Anti-LAMP3 antibody (Alexa-fluor 488)

STJ170004 St John's Laboratory 100 µg 393 EUR
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 546)

STJ170005 St John's Laboratory 100 µg 393 EUR
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 488)

STJ170009 St John's Laboratory 100 µg 393 EUR
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 546)

STJ170010 St John's Laboratory 100 µg 393 EUR
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 488)

STJ170014 St John's Laboratory 100 µg 393 EUR
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 546)

STJ170015 St John's Laboratory 100 µg 393 EUR
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 488)

STJ170020 St John's Laboratory 100 µg 393 EUR
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 546)

STJ170021 St John's Laboratory 100 µg 393 EUR
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-RPSA Alexa Fluor® 488

A4-829-C100 ExBio 0.1 mg 310 EUR

Mouse IgG1-Alexa 647 conjugate (isotype control)

20102-101-A647 Alpha Diagnostics 50 Tests 263 EUR

Anti-Hu CD16 Alexa Fluor® 488

A4-646-T100 ExBio 100 tests 269 EUR

Goat anti Mouse IgG1 (Alexa Fluor 488)

43R-1649 Fitzgerald 500 ug 570 EUR
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)

Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid]

1795 AAT Bioquest 10 mg 393 EUR

AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate]

16890 AAT Bioquest 1 mg 176 EUR

AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]

16891 AAT Bioquest 1 mg 176 EUR

AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]

16892 AAT Bioquest 1 mg 176 EUR

AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin]

23150 AAT Bioquest 300 Tests 306 EUR

AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin]

23153 AAT Bioquest 300 Tests 306 EUR

AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]

23158 AAT Bioquest 300 Tests 306 EUR

Anti-Hu CD72 Alexa Fluor® 488

A4-310-T100 ExBio 100 tests 269 EUR

Anti-Bov CD9 Alexa Fluor® 488

A4-354-C100 ExBio 0.1 mg 269 EUR

Anti-Hu CD30 Alexa Fluor® 700

A7-455-T100 ExBio 100 tests 269 EUR

Anti-Hu CD94 Alexa Fluor® 700

A7-727-T100 ExBio 100 tests 269 EUR

Anti-Hu CD56 Alexa Fluor® 700

A7-789-T100 ExBio 100 tests 269 EUR

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)

43R-ID005AF Fitzgerald 500 ug 338 EUR
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID022AF Fitzgerald 500 ug 364 EUR
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID047AF Fitzgerald 500 ug 462 EUR
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-ID056AF Fitzgerald 500 ug 343 EUR
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594)

Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-IR016AF Fitzgerald 1 mg 281 EUR
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594)

Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated

S0005 Affbiotech 200ul 376 EUR

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated

S0006 Affbiotech 200ul 376 EUR

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated

S0017 Affbiotech 200ul 304 EUR

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated

S0018 Affbiotech 200ul 304 EUR

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated

Ab8011-001 GenDepot 1mg 334 EUR

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated

Ab8032-001 GenDepot 0.5mg 435 EUR

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488

A4-686-C100 ExBio 0.1 mg 269 EUR

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488

A4-712-C100 ExBio 0.1 mg 269 EUR

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488

A4-730-C100 ExBio 0.1 mg 269 EUR

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488

A4-737-C100 ExBio 0.1 mg 269 EUR

Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594)

43R-ID012AF Fitzgerald 300 ug 410 EUR
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594)

XL-647

B2204-1 Biovision 142 EUR

XL-647

B2204-5 Biovision 403 EUR

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488)

M09406 BosterBio 100ug 565 EUR
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488)

M02316 BosterBio 100ug 580 EUR
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human.

gp100 (639–647)

5-01247 CHI Scientific 4 x 5mg Ask for price

Cyanine 647 alkyne

92101 Biotium 1MG 226 EUR
Description: Minimum order quantity: 1 unit of 1MG

Cyanine 647 aminooxy

96008 Biotium 1MG 342 EUR
Description: Minimum order quantity: 1 unit of 1MG

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)

50336 Alpha Diagnostics 0.5 ml 225 EUR

Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)

50337 Alpha Diagnostics 0.5 ml 225 EUR

iFluor™ 647 amine

1074 AAT Bioquest 1 mg 219 EUR

Annexin V Dyomics 647

EXB0023 ExBio 100 tests 222 EUR

Cyanine 647 succinimidyl ester

9-90025 Biotium
  • 1100.00 EUR
  • 226.00 EUR
  • 10MG
  • 1MG
Description: Minimum order quantity: 1 unit of 1MG

gp100 (639-647) Peptide

20-abx266103 Abbexa
  • 411.00 EUR
  • 662.00 EUR
  • 314.00 EUR
  • 10 mg
  • 25 mg
  • 5 mg

hsa-miR-647 Primers

MPH01949 ABM 150 ul / 10 uM 121 EUR

Monoclonal Anti-Monkey IgG-Alexa 647 Conj. (specific for monkey; no reactivity with human or animals IgG)

70030-AF647 Alpha Diagnostics 50 tests 347 EUR

iFluor™ 647-streptavidin conjugate

16966 AAT Bioquest 200 ug 115 EUR

iFluor™ 647-streptavidin conjugate

16996 AAT Bioquest 1 mg 176 EUR

Phalloidin-iFluor™ 647 Conjugate

23127 AAT Bioquest 300 Tests 176 EUR

Cyanine 647-dUTP, lyophilized powder

40065 Biotium 25nmol 236 EUR
Description: Minimum order quantity: 1 unit of 25nmol

hsa-miR-647 miRNA Inhibitor

MIH03303 ABM 2 x 5.0 nmol 176 EUR

hsa-miR-647 miRNA Antagomir

MNH03303 ABM 2 x 5.0 nmol 329 EUR

Anti-Cytokeratins Alexa Fluor488

A4-108-C025 ExBio 0.025 mg 175 EUR

Anti-Cytokeratins Alexa Fluor488

A4-108-C100 ExBio 0.1 mg 310 EUR

Anti-PSMA Alexa Fluor488

A4-539-C025 ExBio 0.025 mg 227 EUR

Anti-PSMA Alexa Fluor488

A4-539-C100 ExBio 0.1 mg 414 EUR

Anti-FoxP3 Alexa Fluor488

A4-601-C025 ExBio 0.025 mg 201 EUR

Anti-FoxP3 Alexa Fluor488

A4-601-C100 ExBio 0.1 mg 362 EUR

Anti-Phosphotyrosine Alexa Fluor647

A6-263-C025 ExBio 0.025 mg 154 EUR

Anti-Phosphotyrosine Alexa Fluor647

A6-263-C100 ExBio 0.1 mg 269 EUR

Anti-LCK Alexa Fluor647

A6-269-C025 ExBio 0.025 mg 206 EUR

Anti-LCK Alexa Fluor647

A6-269-C100 ExBio 0.1 mg 373 EUR

Anti-FoxP3 Alexa Fluor647

A6-601-C025 ExBio 0.025 mg 201 EUR

Anti-FoxP3 Alexa Fluor647

A6-601-C100 ExBio 0.1 mg 362 EUR

Alpha Fluor™ 488 amine

1705 AAT Bioquest 1 mg 306 EUR

Alpha Fluor™ 488 Hydroxylamine

1900 AAT Bioquest 1 mg 306 EUR

Tide Fluor 2-LL-37

H-8286.0100 Bachem 0.1mg 312 EUR
Description: Sum Formula: C205H340N60O53+dye

Tide Fluor 2-LL-37

H-8286.0500 Bachem 0.5mg 1017 EUR
Description: Sum Formula: C205H340N60O53+dye

RPE-iFluor™ 647-streptavidin conjugate

16906 AAT Bioquest 100 ug 219 EUR

Annexin V-iFluor™ 647 conjugate

20074 AAT Bioquest 100 tests 219 EUR

ViaFluor 647 Live Cell Microtubule Stain

70063 Biotium 50uL 439 EUR
Description: Minimum order quantity: 1 unit of 50uL

Anti-Irs4 (mouse aa633-647) antibody

STJ72906 St John's Laboratory 100 µg 359 EUR

Recombinant Hemagglutinin Influenza A Virus H3N2 Wisconsin 67/05

RP-647 Alpha Diagnostics 2 ug 164 EUR

R-Spondin-1 Recombinant Protein

40-647 ProSci 5 ug 259.25 EUR
Description: R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in the adrenal glands, ovary, testis, thyroid, and trachea. Rspo can interact with the Frizzled/LRP6 receptor complex in a manner that stimulates the Wnt/β-catenin signaling pathway. Recombinant Human R-Spondin-1 is a 26.7 kDa protein consisting of 243 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS-PAGE analysis under reducing conditions.

RENIN Recombinant Protein

96-647 ProSci 0.1 mg 569 EUR
Description: Renin is also known as REN and angiotensinogenase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS), and plays an essential role in the elevation of arterial blood pressure and increased sodium retention by the kidney. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. Renin is secreted from kidney cells, which are activated via signaling from the macula densa, which responds to the rate of fluid flow through the distal tubule, by decreases in renal perfusion pressure (through stretch receptors in the vascular wall), and by sympathetic nervous stimulation, mainly through beta-1 receptor activation. Renin can bind to ATP6AP2, which results in a fourfold increase in the conversion of angiotensinogen to angiotensin I over that shown by soluble renin. In addition, renin binding results in phosphorylation of serine and tyrosine residues of ATP6AP2. The level of renin mRNA appears to be modulated by the binding of HADHB, HuR and CP1 to a regulatory region in the 3' UTR. An over-active renin-angiotension system leads to vasoconstriction and retention of sodium and water. These effects lead to hypertension. Therefore, renin inhibitors can be used for the treatment of hypertension.

BMPG Recombinant Protein

91-647 ProSci 0.05 mg 710.75 EUR
Description: Bone Marrow Proteoglycan (BMPG) is a secreted protein that contains one C-type lectin domain. BMPG is the predominant constituent of the crystalline core of the eosinophil granule. BMPG is highly expressed in placenta and pregnancy serum. BMPG may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin. BMPG induces non-cytolytic histamine release from human basophils. In addition, BMPG also participated in antiparasitic defense mechanisms and immune hypersensitivity reactions.

Fc gamma RI Recombinant Protein

92-647 ProSci 0.05 mg 401 EUR
Description: CD64, also known as Fc-gamma receptor 1 (Fc gamma RI), is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity. After binding IgG, CD64 interacts with an accessory chain known as the common gamma chain ( gamma chain), which possesses an ITAM motif that is necessary for triggering cellular activation. CD64 is composed of a signal peptide, three extracellular immunoglobulin domains of the C2-type used to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 mediates endocytosis, phagocytosis, antibody-dependent cellular cytotoxicity, cytokine release, and superoxide production. It is normally expressed on the surfaces of monocytes and macrophages.

Anti-Cytokeratin 18 Alexa Fluor488

A4-106-C025 ExBio 0.025 mg 186 EUR

Anti-Cytokeratin 18 Alexa Fluor488

A4-106-C100 ExBio 0.1 mg 331 EUR

Anti-Cytokeratin 19 Alexa Fluor488

A4-120-C025 ExBio 0.025 mg 186 EUR

Anti-Cytokeratin 19 Alexa Fluor488

A4-120-C100 ExBio 0.1 mg 331 EUR

Anti-Ki-67 Alexa Fluor488

A4-155-T025 ExBio 25 tests 154 EUR

Anti-Ki-67 Alexa Fluor488

A4-155-T100 ExBio 100 tests 269 EUR

Anti-Hu CD45 Alexa Fluor488

A4-160-T100 ExBio 100 tests 269 EUR

Anti-Hu CD193 Alexa Fluor488

A4-161-T100 ExBio 100 tests 269 EUR

Anti-Hu CD279 Alexa Fluor488

A4-176-T100 ExBio 100 tests 269 EUR

Anti-Hu CD43 Alexa Fluor488

A4-220-T025 ExBio 25 tests 154 EUR

Anti-Hu CD43 Alexa Fluor488

A4-220-T100 ExBio 100 tests 269 EUR

Anti-Hu CD44 Alexa Fluor488

A4-221-T025 ExBio 25 tests 154 EUR

Anti-Hu CD44 Alexa Fluor488

A4-221-T100 ExBio 100 tests 269 EUR

Anti-Hu CD45 Alexa Fluor488

A4-222-T025 ExBio 25 tests 154 EUR

Anti-Hu CD45 Alexa Fluor488

A4-222-T100 ExBio 100 tests 269 EUR

Anti-Hu CD55 Alexa Fluor488

A4-230-T025 ExBio 25 tests 154 EUR
The Xc TALome reveals a number of indicators of DNA rearrangements that most likely drove its evolution from two ancestral tal genes. We found that Tal12a and Tal15a of Xcc pressure Xca5 contribute collectively in the event of illness signs on vulnerable B. oleracea var. botrytis cv Clovis. This giant and polymorphic repertoire of TALEs opens novel views for elucidating TALE-mediated susceptibility of Brassicaceae to black rot illness and for understanding the molecular processes underlying TALE evolution.

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Check out the latest CD86 antibodies from
@BosterbioCo

#cd86 #antibodies #polyclonal #monoclonal
#research

Click here
https://www.antibodyresource.com/site/show/company-spotlight/boster-bio#cd86

New #podcast by @DNApodcast

Happy #Pride! 🏳️‍🌈 Celebrate with our FAV @PhenoTips
Speaker Series episode.

#genderaffirming care in #geneticcounseling

Panelists are:
@GenomeMed's @mercadojcgc
@sema4's Marney Brillinger
Patient advocate @holdenmyhorses
https://media.antibodyresource.com/podcast/watch_video.php?v=86RNGGYARDK5

Check out the latest Leptin antibodies from
@BosterbioCo

#antibodies #polyclonal #monoclonal
#research #leptin #Science #ScientificResearch

Click here
https://www.antibodyresource.com/site/show/company-spotlight/boster-bio-1#leptin

Neuronal communications facilitated by SNARE proteins with which synaptic vesicles fuse to the plasma membrane and release the neurotransmitter.

By Ryu, J.K et al.

#neuroscience #medtwiter #Science #Bioinformatics #biochemistry #Neurotwitter

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