Tal Effectors

Transcription activator-like effector nuclease

STAR: a simple TAL effector assembly reaction using isothermal assembly.

STAR: a simple TAL effector assembly reaction using isothermal assembly.
Transcription activator-like effectors (TALEs) include modular programmable DNA binding domains. Fusing TALEs with effector domains creates artificial transcription components (TALE-TFs) or nucleases (TALENs), enabling exact gene manipulations. The development of TALEs stays difficult on account of their repetitive sequences. Here we report a simple TALE assembly reaction (STAR) that permits particular person laboratories to generate a number of TALEs in a facile method.
STAR makes use of an isothermal assembly (‘Gibson assembly’) that’s labour- and cost-effective, accessible, speedy and scalable. A small 68-part fragment library is employed, and the particular TALE repeat sequence is generated inside ~eight hours. Sequence-verified TALENs or TALE-TF plasmids focusing on 17 bp goal sequences might be produced inside three days, with out the necessity for stepwise intermediate plasmid manufacturing.
We reveal the utility of STAR by way of manufacturing of practical TALE-TFs able to activating human SOX2 expression. STAR addresses a few of the shortcomings of current Golden Gate or solid-phase assembly protocols and allows routine manufacturing of TALE-TFs that may complement rising CRISPR/Cas9-based reagents throughout various functions in mammalian stem cell and artificial biology.

Suppression of Xo1-Mediated Disease Resistance in Rice by a Truncated, Non-DNA-Binding TAL Effector of Xanthomonas oryzae.

Delivered into plant cells by kind III secretion from pathogenic Xanthomonas species, TAL (transcription activator-like) effectors are nuclear-localized, DNA-binding proteins that instantly activate particular host genes. Targets embody genes vital for illness, genes that confer resistance, and genes inconsequential to the host-pathogen interplay. TAL effector specificity is encoded by polymorphic repeats of 33-35 amino acids that work together one-to-one with nucleotides within the recognition website.
Activity relies upon additionally on N-terminal sequences vital for DNA binding and C-terminal nuclear localization alerts (NLS) and an acidic activation area (AD). Coding sequences lacking a lot of the N- and C-terminal areas on account of conserved, in-frame deletions are current and annotated as pseudogenes in sequenced strains of Xanthomonas oryzae pv. oryzicola (Xoc) and pv. oryzae (Xoo), which trigger bacterial leaf streak and bacterial blight of rice, respectively. Here we offer proof that these sequences encode proteins we name “truncTALEs,” for “truncated TAL effectors.”
We present that truncTALE Tal2h of Xoc pressure BLS256, and by correlation truncTALEs in different strains, particularly suppress resistance mediated by the Xo1 locus lately described within the heirloom rice selection Carolina Gold. Xo1-mediated resistance is triggered by completely different TAL effectors from various X. oryzae strains, no matter their DNA binding specificity, and doesn’t require the AD. This implies a direct protein-protein slightly than protein-DNA interplay. Similarly, truncTALEs exhibit various predicted DNA recognition specificities.
And, in vitro, Tal2h didn’t bind any of a number of potential recognition websites. Further, a single candidate NLS sequence in Tal2h was dispensable for resistance suppression. Many truncTALEs have one 28 aa repeat, a size not noticed beforehand. Tested in an engineered TAL effector, this repeat required a single base pair deletion within the DNA, suggesting that it or a neighbor disengages. The presence of the 28 aa repeat, nonetheless, was not required for resistance suppression.
TruncTALEs broaden the paradigm for TAL effector-mediated results on vegetation. We suggest that Tal2h and different truncTALEs act as dominant unfavourable ligands for an immune receptor encoded by the Xo1 locus, probably a nucleotide binding, leucine-rich repeat protein. Understanding truncTALE perform and distribution will inform methods for illness management.

TAL Effectors Drive Transcription Bidirectionally in Plants.

TAL effectors delivered by phytopathogenic Xanthomonas species are DNA-sequence-specific transcriptional activators of host susceptibility genes and typically resistance genes. The modularity of DNA recognition by TAL effectors makes them vital additionally as instruments for gene focusing on and genome enhancing. Effector binding parts (EBEs) acknowledged by native TAL effectors in vegetation have been recognized solely on the ahead strand of goal promoters.
Here, we reveal that TAL effectors can drive plant transcription from EBEs on both strand and in each instructions. Furthermore, we present that a native TAL effector from Xanthomonas oryzae pv. oryzicola drives expression of a goal with an EBE on every strand of its promoter. By inserting that promoter and derivatives between two reporter genes oriented face to face, we present that the TAL effector drives expression from both EBE within the respective orientations, and that exercise on the reverse-strand EBE additionally potentiates ahead transcription pushed by exercise on the forward-strand EBE.
 STAR: a simple TAL effector assembly reaction using isothermal assembly.
Our outcomes reveal new modes of motion for TAL effectors, suggesting the potential for but unrecognized targets vital in plant illness, increasing the search area for off-targets of customized TAL effectors, and highlighting the potential of TAL effectors for probing elementary points of plant transcription.

The N6-Position of Adenine Is a Blind Spot for TALEffectors That Enables Effective Binding of Methylated and Fluorophore-Labeled DNA.

Transcription-activator-like effectors (TALEs) are programmable DNA binding proteins broadly used for genome focusing on. TALEs include a number of concatenated repeats, every selectively recognizing one nucleobase by way of a outlined repeat variable diresidue (RVD). Effective use of TALEs requires data about their binding means to epigenetic and different modified nucleobases occurring in goal DNA. However, other than epigenetic cytosine-5 modifications, the binding means of TALEs to modified DNA is unknown.
We right here examine the binding of TALEs to the epigenetic nucleobase N6-methyladenine (6mA) present in prokaryotic and lately additionally eukaryotic genomes. We discover that the pure, adenine (A)-binding RVD NI is insensitive to 6mA. Model-assisted structure-function research reveal lodging of 6mA by RVDs with altered hydrophobic surfaces and talents of hydrogen bonding to the N6-amino group or N7 atom of A.

CY5

HY-D0821 25mg
EUR 1496

PE-Cy5 (Phycoerhthrin-Cy5 Conjugate)

7850-250
EUR 370

Cy5 phosphoramidite

6071 100 umoles
EUR 50
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

DBCO-Cy5

923 1 mg
EUR 132
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Cy5 azide

A8128-25 25 mg
EUR 1732
Description: Sulfo-Cy5 azide is a sulfonated, hydrophilic and highly water soluble dye for Click chemistry. Due to this features, the dye can be used for labeling biomolecules, especially useful for the labeling of delicate proteins and proteins prone to denaturation, purely aqueous conditions.

Cy5 azide

A8128-5 5 mg
EUR 618
Description: Sulfo-Cy5 azide is a sulfonated, hydrophilic and highly water soluble dye for Click chemistry. Due to this features, the dye can be used for labeling biomolecules, especially useful for the labeling of delicate proteins and proteins prone to denaturation, purely aqueous conditions.

CY5-SE

HY-D0819 25mg
EUR 463

CY5-YNE

HY-D0820 10mM/1mL
EUR 786

CY5-N3

HY-D0832 5mg
EUR 533

Mouse anti-Human CD3, PE-Cy5 Conjugated mAb

28006-50Tests 50 Tests
EUR 253

Mouse anti-Human CD4, PE-Cy5 Conjugated mAb

28013-50Tests 50 Tests
EUR 253

Mouse anti-Human CD8, PE-Cy5 Conjugated mAb

28028-50Tests 50 Tests
EUR 253

Mouse anti-Human CD14, PE-Cy5 Conjugated mAb

28053-50Tests 50 Tests
EUR 252

Mouse anti-Human CD19, PE-Cy5 Conjugated mAb

28074-50Tests 50 Tests
EUR 252

Mouse anti-Human CD45, PE-Cy5 Conjugated mAb

28144-50Tests 50 Tests
EUR 253

PE-Cy5 Tandem

2610 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Azido-Cy5 tyramide

11061 1 mg
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

CD117 antibody (CY5)

61R-CD117bMSCY5 100 ug
EUR 413
Description: Rat monoclonal CD117 antibody (CY5)

CD11b antibody (CY5)

61R-CD11baMSCY5 100 ug
EUR 413
Description: Rat monoclonal CD11b antibody (CY5)

CD19 antibody (CY5)

61R-CD19aMSCY5 100 ug
EUR 435
Description: Rat monoclonal CD19 antibody (CY5)

CD19 antibody (CY5)

61R-CD19cHUCY5 100 tests
EUR 435
Description: Mouse monoclonal CD19 antibody (CY5)

CD3e antibody (CY5)

61R-CD3egMSCY 100 ug
EUR 284
Description: Hamster monoclonal CD3e antibody (CY5)

CD3e antibody (CY5)

61R-CD3ehMSCY 100 ug
EUR 284
Description: Rat monoclonal CD3e antibody (CY5)

CD3 antibody (CY5)

61R-CD3gHUCY5 100 tests
EUR 446
Description: Mouse monoclonal CD3 antibody (CY5)

CD45.1 antibody (CY5)

61R-CD45gMSCY5 100 ug
EUR 457
Description: Mouse monoclonal CD45.1 antibody (CY5)

CD45.2 antibody (CY5)

61R-CD45hMSCY5 100 ug
EUR 381
Description: Mouse monoclonal CD45.2 antibody (CY5)

CD45R antibody (CY5)

61R-CD45RcMSCY5 100 ug
EUR 413
Description: Rat monoclonal CD45R antibody (CY5)

CD4 antibody (CY5)

61R-CD4eMSCY 100 ug
EUR 403
Description: Rat monoclonal CD4 antibody (CY5)

CD4 antibody (CY5)

61R-CD4kHUCY5 100 tests
EUR 435
Description: Mouse monoclonal CD4 antibody (CY5)

CD8a antibody (CY5)

61R-CD8abMSCY5 100 ug
EUR 413
Description: Rat monoclonal CD8a antibody (CY5)

CD8a antibody (CY5)

61R-CD8adCKCY5 100 ug
EUR 543
Description: Mouse monoclonal CD8a antibody (CY5)

CD8a antibody (CY5)

61R-CD8aeCKCY5 100 ug
EUR 457
Description: Mouse monoclonal CD8a antibody (CY5)

Cy5-PEG-SH

abx085228-1kDa50mg 1 kDa; 50 mg
EUR 871
  • Shipped within 5-10 working days.

Cy5-PEG-SH

abx085228-2kDa50mg 2 kDa; 50 mg
EUR 913
  • Shipped within 5-10 working days.

Cy5-PEG-SH

abx085228-34kDa50mg 3.4 kDa; 50 mg
EUR 940
  • Shipped within 5-10 working days.

Cy5-PEG-SH

abx085228-5kDa50mg 5 kDa; 50 mg
EUR 940
  • Shipped within 5-10 working days.

Cy5-PEG-COOH

abx085357-10kDa50mg 10 kDa; 50 mg
EUR 499
  • Shipped within 5-10 working days.

Cy5-PEG-COOH

abx085357-20kDa50mg 20 kDa; 50 mg
EUR 499
  • Shipped within 5-10 working days.

Cy5-PEG-COOH

abx085357-2kDa50mg 2 kDa; 50 mg
EUR 535
  • Shipped within 5-10 working days.

Cy5-PEG-COOH

abx085357-5kDa50mg 5 kDa; 50 mg
EUR 535
  • Shipped within 5-10 working days.

Cy5-PEG-Mal

abx085358-10kDa50mg 10 kDa; 50 mg
EUR 546
  • Shipped within 5-10 working days.

Cy5-PEG-Mal

abx085358-1kDa50mg 1 kDa; 50 mg
EUR 546
  • Shipped within 5-10 working days.

Cy5-PEG-Mal

abx085358-2kDa50mg 2 kDa; 50 mg
EUR 570
  • Shipped within 5-10 working days.

Glucagon-Cy5 Peptide

abx267068-1mg 1 mg
EUR 2068
  • Shipped within 1-2 months.

Cy5 NHS ester

A8108-25 25 mg
EUR 746
Description: Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed.

Cy5 NHS ester

A8108-5 5 mg
EUR 293
Description: Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed.

Cy5 carboxylic acid

A8137-25 25 mg
EUR 1036
Description: Sulfo-Cy5 carboxylic acid is a non-activated, sulfonated, hydrophilic and highly water soluble dye. Non-Sulfo-Cy5 carboxylic acid analog is available. For kinds of researches related experiments, using pre-activated sulfo-Cy5 NHS ester is preferred

Cy5 carboxylic acid

A8137-5 5 mg
EUR 525
Description: Sulfo-Cy5 carboxylic acid is a non-activated, sulfonated, hydrophilic and highly water soluble dye. Non-Sulfo-Cy5 carboxylic acid analog is available. For kinds of researches related experiments, using pre-activated sulfo-Cy5 NHS ester is preferred

Annexin V-Cy5 conjugate

20066 100 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Annexin V-Cy5 Reagent

1013-1000
EUR 1132

Annexin V-Cy5 Reagent

1013-200
EUR 403

Cy5®-streptavidin conjugate

16913 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

FMOC-Lys(Cy5)-OH

5055 100 mg
EUR 654
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

CD45RO Antibody (PE-Cy5)

abx134008-100Assays 100 Assays
EUR 495
  • Shipped within 5-10 working days.

CD45RO Antibody (PE-Cy5)

abx134008-50Assays 50 Assays
EUR 398
  • Shipped within 5-10 working days.

PE-Cy5 Calibration Kit

ECFP-F4-5K 5X1 mL
EUR 310
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Cy5 azide (non-sulfonated)

A8113-25 25 mg
EUR 1616
Description: Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction.

Cy5 azide (non-sulfonated)

A8113-5 5 mg
EUR 583
Description: Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction.

Cy5 alkyne (non-sulfonated)

A8131-25 25 mg
EUR 1616
Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore.

Cy5 alkyne (non-sulfonated)

A8131-5 5 mg
EUR 583
Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore.

Cy5 maleimide (non-sulfonated)

A8139-25 25 mg
EUR 525
Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups.

Cy5 maleimide (non-sulfonated)

A8139-5 5 mg
EUR 293
Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups.

Cy5 amine (non-sulfonated)

A8143-25 25 mg
EUR 525
Description: Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents.

Cy5 amine (non-sulfonated)

A8143-5 5 mg
EUR 293
Description: Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents.

Cy5 hydrazide (non-sulfonated)

A8145-25 25 mg
EUR 525
Description: Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones.

Cy5 hydrazide (non-sulfonated)

A8145-5 5 mg
EUR 293
Description: Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones.

Cy5 NHS ester(Et)

A8769-25 25 mg
EUR 525

Cy5 NHS ester(Et)

A8769-5 5 mg
EUR 293

Cy5 Bis NHS ester

A8773-25 25 mg
EUR 525

Cy5 Bis NHS ester

A8773-5 5 mg
EUR 293

Cy5 Bis carboxylic acid

A8775-25 25 mg
EUR 525

Cy5 Bis carboxylic acid

A8775-5 5 mg
EUR 293

Cy5 carboxylic acid(Et)

A8777-25 25 mg
EUR 525

Cy5 carboxylic acid(Et)

A8777-5 5 mg
EUR 293

Cy5-PEG-SH,10K

FL078003-10K-50mg 50mg
EUR 544
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-SH,1K

FL078003-1K-50mg 50mg
EUR 605
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-SH,2K

FL078003-2K-50mg 50mg
EUR 568
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-SH,3.4K

FL078003-3.4K-50mg 50mg
EUR 544
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-SH,5K

FL078003-5K-50mg 50mg
EUR 544
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-COOH,10K

FL078017-10K-50mg 50mg
EUR 470
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-COOH,1K

FL078017-1K-50mg 50mg
EUR 470
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-COOH,20K

FL078017-20K-50mg 50mg
EUR 470
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-COOH,2K

FL078017-2K-50mg 50mg
EUR 470
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-COOH,3.4K

FL078017-3.4K-50mg 50mg
EUR 470
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-COOH,5K

FL078017-5K-50mg 50mg
EUR 470
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-Mal,10K

FL078022-10K-50mg 50mg
EUR 470
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-Mal,1K

FL078022-1K-50mg 50mg
EUR 470
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-Mal,20K

FL078022-20K-50mg 50mg
EUR 470
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-Mal,2K

FL078022-2K-50mg 50mg
EUR 470
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-Mal,3.4K

FL078022-3.4K-50mg 50mg
EUR 470
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Cy5-PEG-Mal,5K

FL078022-5K-50mg 50mg
EUR 470
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Streptavidin: RPE-Cy5 Conjugate

R-1099 0.2 ml Ask for price
Description: fast delivery possible

Annexin V-PE-Cy5 Reagent

1015-1000
EUR 1132

Annexin V-PE-Cy5 Reagent

1015-200
EUR 403

Cy5 NHS ester (non-sulfonated)

A8102-5 5 mg
EUR 293
Description: Cyanine5 NHS ester is a ideal reactive dye for the labeling of peptides, proteins, and oligonucleotides which contain the amino-groups.

Cy5 NHS ester (non-sulfonated)

A8102-5.1 10 mM (in 1mL DMSO)
EUR 525
Description: Cyanine5 NHS ester is a ideal reactive dye for the labeling of peptides, proteins, and oligonucleotides which contain the amino-groups.

Cy5 carboxylic acid (non-sulfonated)

A8133-25 25 mg
EUR 525
Description: Cy5 carboxylic acid is a non-activated carboxylic acid with low aqueous solubility. For coupling with amines, or protein labeling, Cy5 NHS ester and water-soluble sulfo-Cy5 NHS ester is preferred.

Cy5 carboxylic acid (non-sulfonated)

A8133-5 5 mg
EUR 293
Description: Cy5 carboxylic acid is a non-activated carboxylic acid with low aqueous solubility. For coupling with amines, or protein labeling, Cy5 NHS ester and water-soluble sulfo-Cy5 NHS ester is preferred.

Cy5 Boc-hydrazide (non-sulfonated)

A8144-25 25 mg
EUR 525
Description: Boc-protected Cy5 hydrazide is a reactive dye for the labeling of aldehydes and ketones with high aqueous solubility. Trifluoroacetic acid or other strong acid can remove the Boc-(tert-butoxycarbonyl) protective group.

Cy5 Boc-hydrazide (non-sulfonated)

A8144-5 5 mg
EUR 293
Description: Boc-protected Cy5 hydrazide is a reactive dye for the labeling of aldehydes and ketones with high aqueous solubility. Trifluoroacetic acid or other strong acid can remove the Boc-(tert-butoxycarbonyl) protective group.

EZLabel? Antibody Cy5 Labeling Kit

K838-5
EUR 354

EZLabel? Protein Cy5 Labeling Kit

K839-5
EUR 354

Annexin V-Cy5 Apoptosis Kit

K2005-100 100 assays
EUR 516

Annexin V-Cy5 Apoptosis Kit

K2005-25 25 assays
EUR 238

Annexin V-Cy5 Apoptosis Kit

K2005-400 400 assays
EUR 1114

Annexin V-Cy5 Apoptosis Kit

K103-100
EUR 512

Annexin V-Cy5 Apoptosis Kit

K103-25
EUR 218

Annexin V-Cy5 Apoptosis Kit

K103-400
EUR 1023

Anti-Hu CD15 PE-Cy5

T8-138-T100 100 tests
EUR 240

Anti-Hu CD184 PE-Cy5

T8-146-T100 100 tests
EUR 240

Anti-Hu CD45 PE-Cy5

T8-160-T100 100 tests
EUR 240

Anti-Hu CD7 PE-Cy5

T8-183-T025 25 tests
EUR 140

Anti-Hu CD7 PE-Cy5

T8-183-T100 100 tests
EUR 240

Anti-Hu CD8 PE-Cy5

T8-207-T025 25 tests
EUR 140

Anti-Hu CD8 PE-Cy5

T8-207-T100 100 tests
EUR 240

Anti-Hu CD10 PE-Cy5

T8-209-T025 25 tests
EUR 140

Anti-Hu CD10 PE-Cy5

T8-209-T100 100 tests
EUR 240

Anti-Hu CD45 PE-Cy5

T8-222-T025 25 tests
EUR 140

Anti-Hu CD45 PE-Cy5

T8-222-T100 100 tests
EUR 240

Anti-Hu CD55 PE-Cy5

T8-230-T025 25 tests
EUR 140

Anti-Hu CD55 PE-Cy5

T8-230-T100 100 tests
EUR 240

Anti-Hu CD56 PE-Cy5

T8-231-T025 25 tests
EUR 140

Anti-Hu CD56 PE-Cy5

T8-231-T100 100 tests
EUR 240

Anti-Hu CD31 PE-Cy5

T8-273-T025 25 tests
EUR 140

Anti-Hu CD31 PE-Cy5

T8-273-T100 100 tests
EUR 240

Anti-Hu CD80 PE-Cy5

T8-287-T025 25 tests
EUR 140

Anti-Hu CD80 PE-Cy5

T8-287-T100 100 tests
EUR 240

Anti-Hu CD14 PE-Cy5

T8-293-T025 25 tests
EUR 140

Anti-Hu CD14 PE-Cy5

T8-293-T100 100 tests
EUR 240

Anti-Hu CD21 PE-Cy5

T8-306-T025 25 tests
EUR 140

Anti-Hu CD21 PE-Cy5

T8-306-T100 100 tests
EUR 240

Anti-Hu CD27 PE-Cy5

T8-308-T025 25 tests
EUR 140

Anti-Hu CD27 PE-Cy5

T8-308-T100 100 tests
EUR 240

Anti-Hu CD41 PE-Cy5

T8-309-T025 25 tests
EUR 140

Anti-Hu CD41 PE-Cy5

T8-309-T100 100 tests
EUR 240

Anti-Hu IgM PE-Cy5

T8-320-C025 0.025 mg
EUR 126

Anti-Hu IgM PE-Cy5

T8-320-C100 0.1 mg
EUR 213

Anti-Hu IgE PE-Cy5

T8-324-C025 0.025 mg
EUR 126

Anti-Hu IgE PE-Cy5

T8-324-C100 0.1 mg
EUR 213

Anti-Hu CD63 PE-Cy5

T8-343-T025 25 tests
EUR 140

Anti-Hu CD63 PE-Cy5

T8-343-T100 100 tests
EUR 240

Anti-Hu CD4 PE-Cy5

T8-359-T025 25 tests
EUR 140

Anti-Hu CD4 PE-Cy5

T8-359-T100 100 tests
EUR 240

Anti-Hu CD38 PE-Cy5

T8-366-T025 25 tests
EUR 140
Surprisingly, this tolerance of N6 substitution was transferrable to cumbersome N6-alkynyl substituents usable for click on chemistry and even to a massive rhodamine dye, establishing the N6 place of A as the primary website of DNA that gives label introduction inside TALE goal websites with out interference. These findings will information future in vivo research with TALEs and broaden their applicability as DNA seize probes for analytical functions in vitro.

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