Transcription activator-like effectors (TALEs) include modular programmable DNA binding domains. Fusing TALEs with effector domains creates artificial transcription components (TALE-TFs) or nucleases (TALENs), enabling exact gene manipulations. The development of TALEs stays difficult on account of their repetitive sequences. Here we report a simple TALE assembly reaction (STAR) that permits particular person laboratories to generate a number of TALEs in a facile method.
STAR makes use of an isothermal assembly (‘Gibson assembly’) that’s labour- and cost-effective, accessible, speedy and scalable. A small 68-part fragment library is employed, and the particular TALE repeat sequence is generated inside ~eight hours. Sequence-verified TALENs or TALE-TF plasmids focusing on 17 bp goal sequences might be produced inside three days, with out the necessity for stepwise intermediate plasmid manufacturing.
We reveal the utility of STAR by way of manufacturing of practical TALE-TFs able to activating human SOX2 expression. STAR addresses a few of the shortcomings of current Golden Gate or solid-phase assembly protocols and allows routine manufacturing of TALE-TFs that may complement rising CRISPR/Cas9-based reagents throughout various functions in mammalian stem cell and artificial biology.
Suppression of Xo1-Mediated Disease Resistance in Rice by a Truncated, Non-DNA-Binding TAL Effector of Xanthomonas oryzae.
Delivered into plant cells by kind III secretion from pathogenic Xanthomonas species, TAL (transcription activator-like) effectors are nuclear-localized, DNA-binding proteins that instantly activate particular host genes. Targets embody genes vital for illness, genes that confer resistance, and genes inconsequential to the host-pathogen interplay. TAL effector specificity is encoded by polymorphic repeats of 33-35 amino acids that work together one-to-one with nucleotides within the recognition website.
Activity relies upon additionally on N-terminal sequences vital for DNA binding and C-terminal nuclear localization alerts (NLS) and an acidic activation area (AD). Coding sequences lacking a lot of the N- and C-terminal areas on account of conserved, in-frame deletions are current and annotated as pseudogenes in sequenced strains of Xanthomonas oryzae pv. oryzicola (Xoc) and pv. oryzae (Xoo), which trigger bacterial leaf streak and bacterial blight of rice, respectively. Here we offer proof that these sequences encode proteins we name “truncTALEs,” for “truncated TAL effectors.”
We present that truncTALE Tal2h of Xoc pressure BLS256, and by correlation truncTALEs in different strains, particularly suppress resistance mediated by the Xo1 locus lately described within the heirloom rice selection Carolina Gold. Xo1-mediated resistance is triggered by completely different TAL effectors from various X. oryzae strains, no matter their DNA binding specificity, and doesn’t require the AD. This implies a direct protein-protein slightly than protein-DNA interplay. Similarly, truncTALEs exhibit various predicted DNA recognition specificities.
And, in vitro, Tal2h didn’t bind any of a number of potential recognition websites. Further, a single candidate NLS sequence in Tal2h was dispensable for resistance suppression. Many truncTALEs have one 28 aa repeat, a size not noticed beforehand. Tested in an engineered TAL effector, this repeat required a single base pair deletion within the DNA, suggesting that it or a neighbor disengages. The presence of the 28 aa repeat, nonetheless, was not required for resistance suppression.
TruncTALEs broaden the paradigm for TAL effector-mediated results on vegetation. We suggest that Tal2h and different truncTALEs act as dominant unfavourable ligands for an immune receptor encoded by the Xo1 locus, probably a nucleotide binding, leucine-rich repeat protein. Understanding truncTALE perform and distribution will inform methods for illness management.
TAL Effectors Drive Transcription Bidirectionally in Plants.
TAL effectors delivered by phytopathogenic Xanthomonas species are DNA-sequence-specific transcriptional activators of host susceptibility genes and typically resistance genes. The modularity of DNA recognition by TAL effectors makes them vital additionally as instruments for gene focusing on and genome enhancing. Effector binding parts (EBEs) acknowledged by native TAL effectors in vegetation have been recognized solely on the ahead strand of goal promoters.
Here, we reveal that TAL effectors can drive plant transcription from EBEs on both strand and in each instructions. Furthermore, we present that a native TAL effector from Xanthomonas oryzae pv. oryzicola drives expression of a goal with an EBE on every strand of its promoter. By inserting that promoter and derivatives between two reporter genes oriented face to face, we present that the TAL effector drives expression from both EBE within the respective orientations, and that exercise on the reverse-strand EBE additionally potentiates ahead transcription pushed by exercise on the forward-strand EBE.
Our outcomes reveal new modes of motion for TAL effectors, suggesting the potential for but unrecognized targets vital in plant illness, increasing the search area for off-targets of customized TAL effectors, and highlighting the potential of TAL effectors for probing elementary points of plant transcription.
The N6-Position of Adenine Is a Blind Spot for TAL–Effectors That Enables Effective Binding of Methylated and Fluorophore-Labeled DNA.
Transcription-activator-like effectors (TALEs) are programmable DNA binding proteins broadly used for genome focusing on. TALEs include a number of concatenated repeats, every selectively recognizing one nucleobase by way of a outlined repeat variable diresidue (RVD). Effective use of TALEs requires data about their binding means to epigenetic and different modified nucleobases occurring in goal DNA. However, other than epigenetic cytosine-5 modifications, the binding means of TALEs to modified DNA is unknown.
We right here examine the binding of TALEs to the epigenetic nucleobase N6-methyladenine (6mA) present in prokaryotic and lately additionally eukaryotic genomes. We discover that the pure, adenine (A)-binding RVD NI is insensitive to 6mA. Model-assisted structure-function research reveal lodging of 6mA by RVDs with altered hydrophobic surfaces and talents of hydrogen bonding to the N6-amino group or N7 atom of A.
 CY5-SE |
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| HY-D0819 | MedChemExpress | 25mg | EUR 463 |
 CY5-YNE |
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| HY-D0820 | MedChemExpress | 10mM/1mL | EUR 786 |
 CY5-N3 |
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| HY-D0832 | MedChemExpress | 5mg | EUR 533 |
 Cy5 azide |
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| A8128-25 | ApexBio | 25 mg | EUR 1732 |
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Description: Sulfo-Cy5 azide is a sulfonated, hydrophilic and highly water soluble dye for Click chemistry. Due to this features, the dye can be used for labeling biomolecules, especially useful for the labeling of delicate proteins and proteins prone to denaturation, purely aqueous conditions. |
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Cy5 azide |
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| A8128-5 | ApexBio | 5 mg | EUR 618 |
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Description: Sulfo-Cy5 azide is a sulfonated, hydrophilic and highly water soluble dye for Click chemistry. Due to this features, the dye can be used for labeling biomolecules, especially useful for the labeling of delicate proteins and proteins prone to denaturation, purely aqueous conditions. |
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 Mouse anti-Human CD3, PE-Cy5 Conjugated mAb |
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| 28006-50Tests | SAB | 50 Tests | EUR 253 |
 Mouse anti-Human CD4, PE-Cy5 Conjugated mAb |
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| 28013-50Tests | SAB | 50 Tests | EUR 253 |
 Mouse anti-Human CD8, PE-Cy5 Conjugated mAb |
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| 28028-50Tests | SAB | 50 Tests | EUR 253 |
 Mouse anti-Human CD14, PE-Cy5 Conjugated mAb |
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| 28053-50Tests | SAB | 50 Tests | EUR 252 |
 Mouse anti-Human CD19, PE-Cy5 Conjugated mAb |
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| 28074-50Tests | SAB | 50 Tests | EUR 252 |
 Mouse anti-Human CD45, PE-Cy5 Conjugated mAb |
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| 28144-50Tests | SAB | 50 Tests | EUR 253 |
) CD117 antibody (CY5) |
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| 61R-CD117bMSCY5 | Fitzgerald | 100 ug | EUR 413 |
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Description: Rat monoclonal CD117 antibody (CY5) |
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) CD11b antibody (CY5) |
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| 61R-CD11baMSCY5 | Fitzgerald | 100 ug | EUR 413 |
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Description: Rat monoclonal CD11b antibody (CY5) |
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) CD19 antibody (CY5) |
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| 61R-CD19aMSCY5 | Fitzgerald | 100 ug | EUR 435 |
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Description: Rat monoclonal CD19 antibody (CY5) |
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CD19 antibody (CY5) |
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| 61R-CD19cHUCY5 | Fitzgerald | 100 tests | EUR 435 |
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Description: Mouse monoclonal CD19 antibody (CY5) |
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) CD3e antibody (CY5) |
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| 61R-CD3egMSCY | Fitzgerald | 100 ug | EUR 284 |
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Description: Hamster monoclonal CD3e antibody (CY5) |
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CD3e antibody (CY5) |
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| 61R-CD3ehMSCY | Fitzgerald | 100 ug | EUR 284 |
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Description: Rat monoclonal CD3e antibody (CY5) |
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) CD3 antibody (CY5) |
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| 61R-CD3gHUCY5 | Fitzgerald | 100 tests | EUR 446 |
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Description: Mouse monoclonal CD3 antibody (CY5) |
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) CD45.1 antibody (CY5) |
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| 61R-CD45gMSCY5 | Fitzgerald | 100 ug | EUR 457 |
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Description: Mouse monoclonal CD45.1 antibody (CY5) |
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) CD45.2 antibody (CY5) |
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| 61R-CD45hMSCY5 | Fitzgerald | 100 ug | EUR 381 |
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Description: Mouse monoclonal CD45.2 antibody (CY5) |
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) CD45R antibody (CY5) |
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| 61R-CD45RcMSCY5 | Fitzgerald | 100 ug | EUR 413 |
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Description: Rat monoclonal CD45R antibody (CY5) |
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) CD4 antibody (CY5) |
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| 61R-CD4eMSCY | Fitzgerald | 100 ug | EUR 403 |
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Description: Rat monoclonal CD4 antibody (CY5) |
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CD4 antibody (CY5) |
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| 61R-CD4kHUCY5 | Fitzgerald | 100 tests | EUR 435 |
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Description: Mouse monoclonal CD4 antibody (CY5) |
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) CD8a antibody (CY5) |
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| 61R-CD8abMSCY5 | Fitzgerald | 100 ug | EUR 413 |
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Description: Rat monoclonal CD8a antibody (CY5) |
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CD8a antibody (CY5) |
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| 61R-CD8adCKCY5 | Fitzgerald | 100 ug | EUR 543 |
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Description: Mouse monoclonal CD8a antibody (CY5) |
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CD8a antibody (CY5) |
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| 61R-CD8aeCKCY5 | Fitzgerald | 100 ug | EUR 457 |
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Description: Mouse monoclonal CD8a antibody (CY5) |
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 Azido-Cy5 tyramide |
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| 11061 | AAT Bioquest | 1 mg | EUR 306 |
 PE-Cy5 Tandem |
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| 2610 | AAT Bioquest | 1 mg | EUR 219 |
 Glucagon-Cy5 Peptide |
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| abx267068-1mg | Abbexa | 1 mg | EUR 2068 |
 Cy5-PEG-SH |
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| abx085228-1kDa50mg | Abbexa | 1 kDa; 50 mg | EUR 871 |
Cy5-PEG-SH |
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| abx085228-2kDa50mg | Abbexa | 2 kDa; 50 mg | EUR 913 |
Cy5-PEG-SH |
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| abx085228-34kDa50mg | Abbexa | 3.4 kDa; 50 mg | EUR 940 |
Cy5-PEG-SH |
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| abx085228-5kDa50mg | Abbexa | 5 kDa; 50 mg | EUR 940 |
 Cy5-PEG-COOH |
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| abx085357-10kDa50mg | Abbexa | 10 kDa; 50 mg | EUR 499 |
Cy5-PEG-COOH |
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| abx085357-20kDa50mg | Abbexa | 20 kDa; 50 mg | EUR 499 |
Cy5-PEG-COOH |
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| abx085357-2kDa50mg | Abbexa | 2 kDa; 50 mg | EUR 535 |
Cy5-PEG-COOH |
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| abx085357-5kDa50mg | Abbexa | 5 kDa; 50 mg | EUR 535 |
 Cy5-PEG-Mal |
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| abx085358-10kDa50mg | Abbexa | 10 kDa; 50 mg | EUR 546 |
Cy5-PEG-Mal |
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| abx085358-1kDa50mg | Abbexa | 1 kDa; 50 mg | EUR 546 |
Cy5-PEG-Mal |
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| abx085358-2kDa50mg | Abbexa | 2 kDa; 50 mg | EUR 570 |
 Cy5 NHS ester |
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| A8108-25 | ApexBio | 25 mg | EUR 746 |
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Description: Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. |
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Cy5 NHS ester |
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| A8108-5 | ApexBio | 5 mg | EUR 293 |
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Description: Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. |
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 Cy5 carboxylic acid |
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| A8137-25 | ApexBio | 25 mg | EUR 1036 |
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Description: Sulfo-Cy5 carboxylic acid is a non-activated, sulfonated, hydrophilic and highly water soluble dye. Non-Sulfo-Cy5 carboxylic acid analog is available. For kinds of researches related experiments, using pre-activated sulfo-Cy5 NHS ester is preferred |
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Cy5 carboxylic acid |
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| A8137-5 | ApexBio | 5 mg | EUR 525 |
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Description: Sulfo-Cy5 carboxylic acid is a non-activated, sulfonated, hydrophilic and highly water soluble dye. Non-Sulfo-Cy5 carboxylic acid analog is available. For kinds of researches related experiments, using pre-activated sulfo-Cy5 NHS ester is preferred |
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 Annexin V-Cy5 Reagent |
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| 1013-1000 | Biovision | EUR 1132 | |
Annexin V-Cy5 Reagent |
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| 1013-200 | Biovision | EUR 403 | |
 Cy5®-streptavidin conjugate |
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| 16913 | AAT Bioquest | 1 mg | EUR 176 |
 Annexin V-Cy5 conjugate |
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| 20066 | AAT Bioquest | 100 Tests | EUR 219 |
-OH) FMOC-Lys(Cy5)-OH |
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| 5055 | AAT Bioquest | 100 mg | EUR 654 |
) CD45RO Antibody (PE-Cy5) |
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| abx134008-100Assays | Abbexa | 100 Assays | EUR 495 |
CD45RO Antibody (PE-Cy5) |
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| abx134008-50Assays | Abbexa | 50 Assays | EUR 398 |
 PE-Cy5 Calibration Kit |
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| ECFP-F4-5K | Spherotech | 5X1 mL | EUR 310 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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 Cy5-PEG-SH,10K |
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| FL078003-10K-50mg | Biochempeg | 50mg | EUR 544 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-SH,1K |
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| FL078003-1K-50mg | Biochempeg | 50mg | EUR 605 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-SH,2K |
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| FL078003-2K-50mg | Biochempeg | 50mg | EUR 568 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-SH,3.4K |
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| FL078003-3.4K-50mg | Biochempeg | 50mg | EUR 544 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-SH,5K |
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| FL078003-5K-50mg | Biochempeg | 50mg | EUR 544 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-COOH,10K |
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| FL078017-10K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-COOH,1K |
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| FL078017-1K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-COOH,20K |
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| FL078017-20K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-COOH,2K |
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| FL078017-2K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-COOH,3.4K |
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| FL078017-3.4K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-COOH,5K |
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| FL078017-5K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-Mal,10K |
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| FL078022-10K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-Mal,1K |
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| FL078022-1K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-Mal,20K |
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| FL078022-20K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-Mal,2K |
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| FL078022-2K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-Mal,3.4K |
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| FL078022-3.4K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Cy5-PEG-Mal,5K |
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| FL078022-5K-50mg | Biochempeg | 50mg | EUR 470 |
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Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound. |
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 Streptavidin: RPE-Cy5 Conjugate |
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| R-1099 | EpiGentek | 0.2 ml | Ask for price |
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Description: fast delivery possible |
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) Cy5 azide (non-sulfonated) |
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| A8113-25 | ApexBio | 25 mg | EUR 1616 |
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Description: Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. |
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Cy5 azide (non-sulfonated) |
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| A8113-5 | ApexBio | 5 mg | EUR 583 |
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Description: Cy5 azide is a labeling reagent ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. |
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) Cy5 alkyne (non-sulfonated) |
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| A8131-25 | ApexBio | 25 mg | EUR 1616 |
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Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. |
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Cy5 alkyne (non-sulfonated) |
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| A8131-5 | ApexBio | 5 mg | EUR 583 |
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Description: Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. |
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) Cy5 maleimide (non-sulfonated) |
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| A8139-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. |
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Cy5 maleimide (non-sulfonated) |
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| A8139-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. |
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) Cy5 amine (non-sulfonated) |
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| A8143-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. |
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Cy5 amine (non-sulfonated) |
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| A8143-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. |
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) Cy5 hydrazide (non-sulfonated) |
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| A8145-25 | ApexBio | 25 mg | EUR 525 |
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Description: Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones. |
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Cy5 hydrazide (non-sulfonated) |
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| A8145-5 | ApexBio | 5 mg | EUR 293 |
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Description: Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones. |
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) Cy5 NHS ester(Et) |
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| A8769-25 | ApexBio | 25 mg | EUR 525 |
Cy5 NHS ester(Et) |
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| A8769-5 | ApexBio | 5 mg | EUR 293 |
 Cy5 Bis NHS ester |
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| A8773-25 | ApexBio | 25 mg | EUR 525 |
Cy5 Bis NHS ester |
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| A8773-5 | ApexBio | 5 mg | EUR 293 |
 Cy5 Bis carboxylic acid |
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| A8775-25 | ApexBio | 25 mg | EUR 525 |
Cy5 Bis carboxylic acid |
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| A8775-5 | ApexBio | 5 mg | EUR 293 |
) Cy5 carboxylic acid(Et) |
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| A8777-25 | ApexBio | 25 mg | EUR 525 |
Cy5 carboxylic acid(Et) |
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| A8777-5 | ApexBio | 5 mg | EUR 293 |
 Annexin V-PE-Cy5 Reagent |
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| 1015-1000 | Biovision | EUR 1132 | |
Annexin V-PE-Cy5 Reagent |
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| 1015-200 | Biovision | EUR 403 | |
 EZLabel? Antibody Cy5 Labeling Kit |
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| K838-5 | Biovision | EUR 354 | |
 EZLabel? Protein Cy5 Labeling Kit |
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| K839-5 | Biovision | EUR 354 | |
 Annexin V-Cy5 Apoptosis Kit |
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| K103-100 | Biovision | EUR 512 | |
Annexin V-Cy5 Apoptosis Kit |
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| K103-25 | Biovision | EUR 218 | |
Annexin V-Cy5 Apoptosis Kit |
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| K103-400 | Biovision | EUR 1023 | |
 Anti-Hu CD15 PE-Cy5 |
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| T8-138-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD184 PE-Cy5 |
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| T8-146-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD45 PE-Cy5 |
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| T8-160-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD7 PE-Cy5 |
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| T8-183-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD7 PE-Cy5 |
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| T8-183-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD8 PE-Cy5 |
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| T8-207-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD8 PE-Cy5 |
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| T8-207-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD10 PE-Cy5 |
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| T8-209-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD10 PE-Cy5 |
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| T8-209-T100 | ExBio | 100 tests | EUR 240 |
Anti-Hu CD45 PE-Cy5 |
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| T8-222-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD45 PE-Cy5 |
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| T8-222-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD55 PE-Cy5 |
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| T8-230-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD55 PE-Cy5 |
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| T8-230-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD56 PE-Cy5 |
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| T8-231-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD56 PE-Cy5 |
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| T8-231-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD31 PE-Cy5 |
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| T8-273-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD31 PE-Cy5 |
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| T8-273-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD80 PE-Cy5 |
|||
| T8-287-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD80 PE-Cy5 |
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| T8-287-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD14 PE-Cy5 |
|||
| T8-293-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD14 PE-Cy5 |
|||
| T8-293-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD21 PE-Cy5 |
|||
| T8-306-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD21 PE-Cy5 |
|||
| T8-306-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD27 PE-Cy5 |
|||
| T8-308-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD27 PE-Cy5 |
|||
| T8-308-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD41 PE-Cy5 |
|||
| T8-309-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD41 PE-Cy5 |
|||
| T8-309-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu IgM PE-Cy5 |
|||
| T8-320-C025 | ExBio | 0.025 mg | EUR 126 |
Anti-Hu IgM PE-Cy5 |
|||
| T8-320-C100 | ExBio | 0.1 mg | EUR 213 |
 Anti-Hu IgE PE-Cy5 |
|||
| T8-324-C025 | ExBio | 0.025 mg | EUR 126 |
Anti-Hu IgE PE-Cy5 |
|||
| T8-324-C100 | ExBio | 0.1 mg | EUR 213 |
 Anti-Hu CD63 PE-Cy5 |
|||
| T8-343-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD63 PE-Cy5 |
|||
| T8-343-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD4 PE-Cy5 |
|||
| T8-359-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD4 PE-Cy5 |
|||
| T8-359-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD38 PE-Cy5 |
|||
| T8-366-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD38 PE-Cy5 |
|||
| T8-366-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD3 PE-Cy5 |
|||
| T8-514-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD3 PE-Cy5 |
|||
| T8-514-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD86 PE-Cy5 |
|||
| T8-531-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD86 PE-Cy5 |
|||
| T8-531-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD117 PE-Cy5 |
|||
| T8-586-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD117 PE-Cy5 |
|||
| T8-586-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD158d PE-Cy5 |
|||
| T8-609-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD20 PE-Cy5 |
|||
| T8-638-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD20 PE-Cy5 |
|||
| T8-638-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD64 PE-Cy5 |
|||
| T8-644-T025 | ExBio | 25 tests | EUR 140 |
Anti-Hu CD64 PE-Cy5 |
|||
| T8-644-T100 | ExBio | 100 tests | EUR 240 |
 Anti-Hu CD16 PE-Cy5 |
|||
| T8-646-T100 | ExBio | 100 tests | EUR 240 |
Surprisingly, this tolerance of N6 substitution was transferrable to cumbersome N6-alkynyl substituents usable for click on chemistry and even to a massive rhodamine dye, establishing the N6 place of A as the primary website of DNA that gives label introduction inside TALE goal websites with out interference. These findings will information future in vivo research with TALEs and broaden their applicability as DNA seize probes for analytical functions in vitro.
