Monday, 20 May, 2024

Tal Effectors

Transcription activator-like effector nuclease

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  • STAR: a simple TAL effector assembly reaction using isothermal assembly.
STAR: a simple TAL effector assembly reaction using isothermal assembly.
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STAR: a simple TAL effector assembly reaction using isothermal assembly.

Transcription activator-like effectors (TALEs) include modular programmable DNA binding domains. Fusing TALEs with effector domains creates artificial transcription components (TALE-TFs) or nucleases (TALENs), enabling exact gene manipulations. The development of TALEs stays difficult on account of their repetitive sequences. Here we report a simple TALE assembly reaction (STAR) that permits particular person laboratories to generate a number of TALEs in a facile method.
STAR makes use of an isothermal assembly (‘Gibson assembly’) that’s labour- and cost-effective, accessible, speedy and scalable. A small 68-part fragment library is employed, and the particular TALE repeat sequence is generated inside ~eight hours. Sequence-verified TALENs or TALE-TF plasmids focusing on 17 bp goal sequences might be produced inside three days, with out the necessity for stepwise intermediate plasmid manufacturing.
We reveal the utility of STAR by way of manufacturing of practical TALE-TFs able to activating human SOX2 expression. STAR addresses a few of the shortcomings of current Golden Gate or solid-phase assembly protocols and allows routine manufacturing of TALE-TFs that may complement rising CRISPR/Cas9-based reagents throughout various functions in mammalian stem cell and artificial biology.

Suppression of Xo1-Mediated Disease Resistance in Rice by a Truncated, Non-DNA-Binding TAL Effector of Xanthomonas oryzae.

Delivered into plant cells by kind III secretion from pathogenic Xanthomonas species, TAL (transcription activator-like) effectors are nuclear-localized, DNA-binding proteins that instantly activate particular host genes. Targets embody genes vital for illness, genes that confer resistance, and genes inconsequential to the host-pathogen interplay. TAL effector specificity is encoded by polymorphic repeats of 33-35 amino acids that work together one-to-one with nucleotides within the recognition website.
Activity relies upon additionally on N-terminal sequences vital for DNA binding and C-terminal nuclear localization alerts (NLS) and an acidic activation area (AD). Coding sequences lacking a lot of the N- and C-terminal areas on account of conserved, in-frame deletions are current and annotated as pseudogenes in sequenced strains of Xanthomonas oryzae pv. oryzicola (Xoc) and pv. oryzae (Xoo), which trigger bacterial leaf streak and bacterial blight of rice, respectively. Here we offer proof that these sequences encode proteins we name “truncTALEs,” for “truncated TAL effectors.”
We present that truncTALE Tal2h of Xoc pressure BLS256, and by correlation truncTALEs in different strains, particularly suppress resistance mediated by the Xo1 locus lately described within the heirloom rice selection Carolina Gold. Xo1-mediated resistance is triggered by completely different TAL effectors from various X. oryzae strains, no matter their DNA binding specificity, and doesn’t require the AD. This implies a direct protein-protein slightly than protein-DNA interplay. Similarly, truncTALEs exhibit various predicted DNA recognition specificities.
And, in vitro, Tal2h didn’t bind any of a number of potential recognition websites. Further, a single candidate NLS sequence in Tal2h was dispensable for resistance suppression. Many truncTALEs have one 28 aa repeat, a size not noticed beforehand. Tested in an engineered TAL effector, this repeat required a single base pair deletion within the DNA, suggesting that it or a neighbor disengages. The presence of the 28 aa repeat, nonetheless, was not required for resistance suppression.
TruncTALEs broaden the paradigm for TAL effector-mediated results on vegetation. We suggest that Tal2h and different truncTALEs act as dominant unfavourable ligands for an immune receptor encoded by the Xo1 locus, probably a nucleotide binding, leucine-rich repeat protein. Understanding truncTALE perform and distribution will inform methods for illness management.

TAL Effectors Drive Transcription Bidirectionally in Plants.

TAL effectors delivered by phytopathogenic Xanthomonas species are DNA-sequence-specific transcriptional activators of host susceptibility genes and typically resistance genes. The modularity of DNA recognition by TAL effectors makes them vital additionally as instruments for gene focusing on and genome enhancing. Effector binding parts (EBEs) acknowledged by native TAL effectors in vegetation have been recognized solely on the ahead strand of goal promoters.
Here, we reveal that TAL effectors can drive plant transcription from EBEs on both strand and in each instructions. Furthermore, we present that a native TAL effector from Xanthomonas oryzae pv. oryzicola drives expression of a goal with an EBE on every strand of its promoter. By inserting that promoter and derivatives between two reporter genes oriented face to face, we present that the TAL effector drives expression from both EBE within the respective orientations, and that exercise on the reverse-strand EBE additionally potentiates ahead transcription pushed by exercise on the forward-strand EBE.
 STAR: a simple TAL effector assembly reaction using isothermal assembly.
Our outcomes reveal new modes of motion for TAL effectors, suggesting the potential for but unrecognized targets vital in plant illness, increasing the search area for off-targets of customized TAL effectors, and highlighting the potential of TAL effectors for probing elementary points of plant transcription.

The N6-Position of Adenine Is a Blind Spot for TALEffectors That Enables Effective Binding of Methylated and Fluorophore-Labeled DNA.

Transcription-activator-like effectors (TALEs) are programmable DNA binding proteins broadly used for genome focusing on. TALEs include a number of concatenated repeats, every selectively recognizing one nucleobase by way of a outlined repeat variable diresidue (RVD). Effective use of TALEs requires data about their binding means to epigenetic and different modified nucleobases occurring in goal DNA. However, other than epigenetic cytosine-5 modifications, the binding means of TALEs to modified DNA is unknown.
We right here examine the binding of TALEs to the epigenetic nucleobase N6-methyladenine (6mA) present in prokaryotic and lately additionally eukaryotic genomes. We discover that the pure, adenine (A)-binding RVD NI is insensitive to 6mA. Model-assisted structure-function research reveal lodging of 6mA by RVDs with altered hydrophobic surfaces and talents of hydrogen bonding to the N6-amino group or N7 atom of A.

Myc-Tag Monoclonal Antibody(3E8), Cy5 Conjugated

E44H11716 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

β-actin Monoclonal Antibody(5B7), Cy5 Conjugated

E44H11786 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Flag-Tag Monoclonal Antibody(2C5), Cy5 Conjugated

E44H11628 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

β-Tubulin Monoclonal Antibody(5G3), Cy5 Conjugated

E44H11797 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

α-tubulin Monoclonal Antibody(8F11), Cy5 Conjugated

E44H11774 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Cyclophilin B Monoclonal Antibody(2B10), Cy5 Conjugated

E44H11617 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

VSV-G-Tag Monoclonal Antibody(8D6), Cy5 Conjugated

E44H11762 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Cy5 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

SC016 ABM 250 µg 145 EUR

Cy5 Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat]

SC012 ABM 250 µg 145 EUR

Cy5 Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey]

SC004 ABM 250 µg 145 EUR

Cy5 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]

SC014 ABM 250 µg 145 EUR

Cy5 Conjugated Affinity Purified anti-Hamster IgG [H&L] [Goat]

SC006 ABM 250 µg 145 EUR

Cy5 Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey]

SC008 ABM 250 µg 145 EUR

Mouse anti-Human CD3, PE-Cy5 Conjugated mAb

28006-50Tests SAB 50 Tests 303.6 EUR

Mouse anti-Human CD4, PE-Cy5 Conjugated mAb

28013-50Tests SAB 50 Tests 303.6 EUR

Mouse anti-Human CD8, PE-Cy5 Conjugated mAb

28028-50Tests SAB 50 Tests 303.6 EUR

Mouse anti-Human CD3, PE-Cy5 Conjugated mAb.

E16HC003-050 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD3, PE-Cy5 Conjugated mAb.

E16HC003-100 EnoGene 50Tests 225 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD4, PE-Cy5 Conjugated mAb.

E16HC004-050 EnoGene 50Tests 225 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD4, PE-Cy5 Conjugated mAb.

E16HC004-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD4, PE-Cy5 Conjugated mAb.

E16HC0041-050 EnoGene 50Tests 225 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD4, PE-Cy5 Conjugated mAb.

E16HC0041-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD8, PE-Cy5 Conjugated mAb.

E16HC008-050 EnoGene 50Tests 225 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD8, PE-Cy5 Conjugated mAb.

E16HC008-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD14, PE-Cy5 Conjugated mAb

28053-50Tests SAB 50 Tests 302.4 EUR

Mouse anti-Human CD19, PE-Cy5 Conjugated mAb

28074-50Tests SAB 50 Tests 302.4 EUR

Mouse anti-Human CD45, PE-Cy5 Conjugated mAb

28144-50Tests SAB 50 Tests 303.6 EUR

Mouse anti-Human CD14, PE-Cy5 Conjugated mAb.

E16HC0142-050 EnoGene 50Tests 225 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD14, PE-Cy5 Conjugated mAb.

E16HC0142-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD19, PE-Cy5 Conjugated mAb.

E16HC019-050 EnoGene 50Tests 225 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD19, PE-Cy5 Conjugated mAb.

E16HC019-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD20, PE-Cy5 Conjugated mAb.

E16HC020-050 EnoGene 50Tests 225 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD20, PE-Cy5 Conjugated mAb.

E16HC020-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD25, PE-Cy5 Conjugated mAb.

E16HC0251-050 EnoGene 50Tests 225 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD25, PE-Cy5 Conjugated mAb.

E16HC0251-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD34, PE-Cy5 Conjugated mAb.

E16HC0341-050 EnoGene 50Tests 225 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD34, PE-Cy5 Conjugated mAb.

E16HC0341-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD38, PE-Cy5 Conjugated mAb.

E16HC0381-050 EnoGene 50Tests 225 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD38, PE-Cy5 Conjugated mAb.

E16HC0381-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD40, PE-Cy5 Conjugated mAb.

E16HC040-050 EnoGene 50Tests 225 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD40, PE-Cy5 Conjugated mAb.

E16HC040-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD41, PE-Cy5 Conjugated mAb.

E16HC0411-050 EnoGene 50Tests 225 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD41, PE-Cy5 Conjugated mAb.

E16HC0411-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD45, PE-Cy5 Conjugated mAb.

E16HC045-050 EnoGene 50Tests 225 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD45, PE-Cy5 Conjugated mAb.

E16HC045-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse IgM Isotype Control, PE-Cy5 Conjugated mAb

E16MCC004-050 EnoGene 50Tests 145 EUR
Description: Available in various conjugation types.

Mouse IgM Isotype Control, PE-Cy5 Conjugated mAb

E16MCC004-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse IgG1 Isotype Control, PE-Cy5 Conjugated mAb

E16MCC001-050 EnoGene 50Tests 145 EUR
Description: Available in various conjugation types.

Mouse IgG1 Isotype Control, PE-Cy5 Conjugated mAb

E16MCC001-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD45RO, PE-Cy5 Conjugated mAb.

E16HC0456-050 EnoGene 50Tests 225 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD45RO, PE-Cy5 Conjugated mAb.

E16HC0456-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Streptavidin::Cy5 Conjugate

40180009-1 Glycomatrix 1 mg 147.68 EUR

Mouse IgG2a Isotype Control, PE-Cy5 Conjugated mAb

E16MCC002-050 EnoGene 50Tests 145 EUR
Description: Available in various conjugation types.

Mouse IgG2a Isotype Control, PE-Cy5 Conjugated mAb

E16MCC002-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Mouse IgG2b Isotype Control, PE-Cy5 Conjugated mAb

E16MCC003-050 EnoGene 50Tests 145 EUR
Description: Available in various conjugation types.

Mouse IgG2b Isotype Control, PE-Cy5 Conjugated mAb

E16MCC003-100 EnoGene 100Tests 255 EUR
Description: Available in various conjugation types.

Cy5 biotin conjugate

3100-5mg AAT Bioquest 5 mg 222 EUR
Description: This bifunctional Cy5 biotin conjugate demonstrates excellent avidin binding and strong fluorescence.

Mouse anti-Human CD4/CD8/CD3, FITC/PE/PE-Cy5 Conjugated mAb

28249-20Tests SAB 20 Tests 302.4 EUR

Mouse anti-Human CD4/CD8/CD3, FITC/PE/PE-Cy5 Conjugated mAb

E16HT001-020 EnoGene 50Tests 145 EUR
Description: Available in various conjugation types.

Mouse anti-Human CD4/CD8/CD3, FITC/PE/PE-Cy5 Conjugated mAb

E16HT001-050 EnoGene 100Tests 225 EUR
Description: Available in various conjugation types.

Mouse Anti-Human CD3/CD19/CD45, FITC/PE/PE-Cy5 Conjugated mAb

28252-20Tests SAB 20 Tests 302.4 EUR

PE-Cy5 (Phycoerhthrin-Cy5 Conjugate)

7850-250 Biovision each 444 EUR

Cy3 Conjugated Goat Anti-Mouse IgG

TA130012 Origene Technologies GmbH 500 µg Ask for price

Cy3 Conjugated Goat Anti-Rabbit IgG

TA130020 Origene Technologies GmbH 500 µg Ask for price

Cy3 Conjugated Rabbit Anti-Goat IgG

TA130028 Origene Technologies GmbH 500 µg Ask for price

Cy3 Conjugated Rabbit Anti-Human IgG

TA130044 Origene Technologies GmbH 500 µg Ask for price

Cy3 Conjugated anti-Rat IgG SABC Kit

SA1079 BosterBio 1 kit 426 EUR

Cy3 Conjugated anti-Goat IgG SABC Kit

SA1076 BosterBio 1 kit 426 EUR

Cy3 Conjugated anti-Mouse IgG SABC Kit

SA1072 BosterBio 1 kit 426 EUR

Cy3 Conjugated anti-Mouse IgM SABC Kit

SA1073 BosterBio 1 kit 450 EUR

Cy3 Conjugated anti-Human IgG SABC Kit

SA1078 BosterBio 1 kit 426 EUR

Cy3 Conjugated anti-Rabbit IgG SABC Kit

SA1074 BosterBio 1 kit 426 EUR

GAPDH Monoclonal Antibody(2B8), Cy3 Conjugated

E44H11643 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Histone H3 Monoclonal Antibody, Cy3 Conjugated

E44H11681 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

PCNA Monoclonal Antibody(12D10), Cy3 Conjugated

E44H11728 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

COX IV Monoclonal Antibody(6C8), Cy3 Conjugated

E44H11604 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

V5-Tag Monoclonal Antibody(3C8), Cy3 Conjugated

E44H11750 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

HA-Tag Monoclonal Antibody(1B10), Cy3 Conjugated

E44H11657 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

His-Tag Monoclonal Antibody(4E6), Cy3 Conjugated

E44H11668 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Lamin B1 Monoclonal Antibody(7C11), Cy3 Conjugated

E44H11700 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Myc-Tag Monoclonal Antibody(3E8), Cy3 Conjugated

E44H11715 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

β-actin Monoclonal Antibody(5B7), Cy3 Conjugated

E44H11785 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Flag-Tag Monoclonal Antibody(2C5), Cy3 Conjugated

E44H11627 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

β-Tubulin Monoclonal Antibody(5G3), Cy3 Conjugated

E44H11796 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

α-tubulin Monoclonal Antibody(8F11), Cy3 Conjugated

E44H11773 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Goat anti-Human IgG(H+L) ,Cross absorbed against mouse immunoglobulinsHRPO ConjugatedHRPO ConjugatedHRPO Conjugated HRPO Conjugated HRPO ConjugatedHRPO Conjugated

E16SPH1031-100 EnoGene 100μl 145 EUR

Goat anti-Human IgG(H+L) ,Cross absorbed against mouse immunoglobulinsHRPO ConjugatedHRPO ConjugatedHRPO Conjugated HRPO Conjugated HRPO ConjugatedHRPO Conjugated

E16SPH1031-1000 EnoGene 1000μl 295 EUR

Goat anti-Human IgG(H+L) ,Cross absorbed against mouse immunoglobulinsHRPO ConjugatedHRPO ConjugatedHRPO Conjugated HRPO Conjugated HRPO ConjugatedHRPO Conjugated

E16SPH1031-500 EnoGene 500μl 225 EUR

VSV-G-Tag Monoclonal Antibody(8D6), Cy3 Conjugated

E44H11761 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Annexin V-Cy5 conjugate

20066-100Tests AAT Bioquest 100 Tests 222 EUR
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

Cyclophilin B Monoclonal Antibody(2B10), Cy3 Conjugated

E44H11616 EnoGene 100ul 255 EUR
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Goat anti-Rabbit IgG(H+L), Cross absorbed against human immunoglobulinsHRPO Conjugated HRPO Conjugated HRPO ConjugatedHRPO Conjugated

E16SPH1021-100 EnoGene 100μl 145 EUR

Goat anti-Rabbit IgG(H+L), Cross absorbed against human immunoglobulinsHRPO Conjugated HRPO Conjugated HRPO ConjugatedHRPO Conjugated

E16SPH1021-1000 EnoGene 1000μl 295 EUR

Goat anti-Rabbit IgG(H+L), Cross absorbed against human immunoglobulinsHRPO Conjugated HRPO Conjugated HRPO ConjugatedHRPO Conjugated

E16SPH1021-500 EnoGene 500μl 225 EUR

Goat Anti-Human IgG Secondary Antibody Cy3 Conjugated

L30211 SAB 1.0 ml 85 EUR

Goat Anti-Mouse IgG Secondary Antibody Cy3 Conjugated

L30311 SAB 1.0 ml 85 EUR

RatioWorks™ Cal-590L®/Cy5-Dextran Conjugate *MW 10,000*

20527 AAT Bioquest 1mg 516 EUR
Description: Calcium measurement is critical for numerous biological investigations.

RatioWorks™ Cal-590L®/Cy5-Dextran Conjugate *MW 10,000*

20527-1mg AAT Bioquest 1 mg 505 EUR
Description: Calcium measurement is critical for numerous biological investigations.

RatioWorks™ Cal-520L®/Cy5-Dextran Conjugate *MW 10,000*

20603 AAT Bioquest 1 mg 570 EUR
Description: Calcium measurement is critical for numerous biological investigations.

RatioWorks™ Cal-520L®/Cy5-Dextran Conjugate *MW 10,000*

20603-1mg AAT Bioquest 1 mg 558 EUR
Description: Calcium measurement is critical for numerous biological investigations.

Rabbit anti-Rat IgG (H+L) I,Cross absorbed against human and mouse immunoglobulinsHRPO ConjugatedHRPO ConjugatedHRPO ConjugatedHRPO Conjugated HRPO Conjugated HRPO ConjugatedHRPO Conjugated

E16SPH2011-100 EnoGene 100μl 145 EUR

Rabbit anti-Rat IgG (H+L) I,Cross absorbed against human and mouse immunoglobulinsHRPO ConjugatedHRPO ConjugatedHRPO ConjugatedHRPO Conjugated HRPO Conjugated HRPO ConjugatedHRPO Conjugated

E16SPH2011-1000 EnoGene 1000μl 295 EUR

Rabbit anti-Rat IgG (H+L) I,Cross absorbed against human and mouse immunoglobulinsHRPO ConjugatedHRPO ConjugatedHRPO ConjugatedHRPO Conjugated HRPO Conjugated HRPO ConjugatedHRPO Conjugated

E16SPH2011-500 EnoGene 500μl 225 EUR

Goat anti-Rabbit IgG(H+L) ,Cross absorbed against human and mouse immunoglobulinsHRPO ConjugatedHRPO Conjugated HRPO Conjugated HRPO ConjugatedHRPO Conjugated

E16SPH1022-100 EnoGene 100μl 145 EUR

Goat anti-Rabbit IgG(H+L) ,Cross absorbed against human and mouse immunoglobulinsHRPO ConjugatedHRPO Conjugated HRPO Conjugated HRPO ConjugatedHRPO Conjugated

E16SPH1022-1000 EnoGene 1000μl 295 EUR

Goat anti-Rabbit IgG(H+L) ,Cross absorbed against human and mouse immunoglobulinsHRPO ConjugatedHRPO Conjugated HRPO Conjugated HRPO ConjugatedHRPO Conjugated

E16SPH1022-500 EnoGene 500μl 225 EUR

Goat Anti-Rabbit IgG Secondary Antibody Cy3 Conjugated

L30111 SAB 1.0 ml 85 EUR

Cy2 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

SC015 ABM 250 µg 145 EUR

Cy3 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

SC025 ABM 250 µg 145 EUR

GFP (HRP conjugated)

E8ET1702-69 EnoGene 100ul 275 EUR
Description: Available in various conjugation types.

Streptavidin: RPE-Cy5 Conjugate

R-1099 EpiGentek 0.2 ml Ask for price

2',3'-cGAMP-Cy5 conjugate

20318 AAT Bioquest 100 ug 1009 EUR
Description: 2',3'-cGAMP or cGAMP (Cyclic guanosine monophosphate"adenosine monophosphate) is produced in mammalian cells by cGAS (cGAMP synthase) from ATP and GTP upon cytosolic DNA stimulation.

2',3'-cGAMP-Cy5 conjugate

20318-100ug AAT Bioquest 100 ug 989 EUR
Description: 2',3'-cGAMP or cGAMP (Cyclic guanosine monophosphate"adenosine monophosphate) is produced in mammalian cells by cGAS (cGAMP synthase) from ATP and GTP upon cytosolic DNA stimulation.

Cy3 Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat]

SC023 ABM 250 µg 145 EUR

RPE-Cy5-streptavidin conjugate

16918 AAT Bioquest 100 ug 101 EUR
Description: Streptavidin conjugates are widely used together with a biotin conjugate for specific detection of a variety of biological molecules since streptavidin has a very high binding affinity for biotin and its conjugates.

RPE-Cy5-streptavidin conjugate

16918-100ug AAT Bioquest 100 ug 99 EUR
Description: Streptavidin conjugates are widely used together with a biotin conjugate for specific detection of a variety of biological molecules since streptavidin has a very high binding affinity for biotin and its conjugates.

RPE-Cy5-streptavidin conjugate

16919 AAT Bioquest 1 mg 315 EUR
Description: Streptavidin conjugates are widely used together with a biotin conjugate for specific detection of a variety of biological molecules since streptavidin has a very high binding affinity for biotin and its conjugates.

RPE-Cy5-streptavidin conjugate

16919-1mg AAT Bioquest 1 mg 308 EUR
Description: Streptavidin conjugates are widely used together with a biotin conjugate for specific detection of a variety of biological molecules since streptavidin has a very high binding affinity for biotin and its conjugates.

GAPDH (HRP Conjugated)

E11-12057 EnoGene 100ul 255 EUR
Description: Available in various conjugation types.

GAPDH (HRP conjugated)

E8ET1702-66 EnoGene 100ul 275 EUR
Description: Available in various conjugation types.

Cy2 Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey]

SC002 ABM 250 µg 145 EUR

Cy2 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]

SC013 ABM 250 µg 145 EUR

Cy3 Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey]

SC017 ABM 250 µg 145 EUR

Cy3 Conjugated Affinity Purified anti-Rabbit IgG (H&L) [Goat]

SC024 ABM 250 µg 145 EUR

HRP Conjugated Avidin

BA1081-0.5 BosterBio 0.5ml 175.2 EUR

HRP Conjugated Avidin

BA1081-1 BosterBio 1ml 279.6 EUR

BSA Conjugated Amylin

CPA812Hu11 Cloud-Clone 10ug 120 EUR

OVA Conjugated Amylin

CPA812Hu21 Cloud-Clone 10ug 120 EUR

OVA Conjugated Amylin

CPA812Hu22 Cloud-Clone 10ug 140 EUR

KLH Conjugated Amylin

CPA812Hu33 Cloud-Clone 10ug 132 EUR

BSA Conjugated Amylin

CPA812Mu11 Cloud-Clone 10ug 120 EUR

OVA Conjugated Amylin

CPA812Mu21 Cloud-Clone 10ug 120 EUR

BSA Conjugated Amylin

CPA812Ra11 Cloud-Clone 10ug 120 EUR

OVA Conjugated Amylin

CPA812Ra21 Cloud-Clone 10ug 120 EUR

OVA Conjugated Amylin

RPU50817-100ug Biomatik Corporation 100ug 431.2 EUR

OVA Conjugated Amylin

RPU50817-1mg Biomatik Corporation 1mg 1911 EUR

OVA Conjugated Amylin

RPU50817-50ug Biomatik Corporation 50ug 336 EUR

BSA Conjugated Amylin

RPU50156-100ug Biomatik Corporation 100ug 475.2 EUR

BSA Conjugated Amylin

RPU50156-1mg Biomatik Corporation 1mg 1872 EUR

BSA Conjugated Amylin

RPU50156-50ug Biomatik Corporation 50ug 288 EUR

OVA Conjugated Amylin

RPU50157-100ug Biomatik Corporation 100ug 475.2 EUR

OVA Conjugated Amylin

RPU50157-1mg Biomatik Corporation 1mg 1872 EUR

OVA Conjugated Amylin

RPU50157-50ug Biomatik Corporation 50ug 288 EUR

BSA Conjugated Amylin

RPU50158-100ug Biomatik Corporation 100ug 475.2 EUR
Surprisingly, this tolerance of N6 substitution was transferrable to cumbersome N6-alkynyl substituents usable for click on chemistry and even to a massive rhodamine dye, establishing the N6 place of A as the primary website of DNA that gives label introduction inside TALE goal websites with out interference. These findings will information future in vivo research with TALEs and broaden their applicability as DNA seize probes for analytical functions in vitro.

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