Thursday, 18 July, 2024

Tal Effectors

Transcription activator-like effector nuclease

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  • SWEET genes and TAL effectors for disease resistance in plants: Present status and future prospects
SWEET genes and TAL effectors for disease resistance in plants: Present status and future prospects
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SWEET genes and TAL effectors for disease resistance in plants: Present status and future prospects

SWEET genes encode sugar transporter proteins and usually perform as susceptibility (S) genes. Consequently, the recessive alleles of those SWEET genes present resistance. This evaluate summarizes the accessible literature on the molecular foundation of the position of SWEET genes (as S genes) in the host and corresponding transcription activator-like effectors (TALEs) secreted by the pathogen. The evaluate has 4 main sections, which comply with a quick introduction:
The first half provides some particulars in regards to the incidence and evolution of SWEET genes in roughly 30 plant species; the second half provides some particulars about programs the place (a) SWEET genes with and with out TALEs and (b) TALEs with out SWEET genes trigger completely different illnesses; the third half summarizes the accessible details about TALEs together with interfering/truncated TALEs secreted by the pathogens; this part additionally summarizes the accessible data on effector-binding components (EBEs) accessible in the promoters of both the SWEET genes or the Executor R genes; the code that’s used for binding of TALEs to EBEs can be described in this part; the fourth half provides some particulars in regards to the accessible approaches which can be getting used or can be utilized in the future for exploiting SWEET genes for creating disease-resistant cultivars.
The evaluate concludes with a piece giving conclusions and future potentialities of utilizing SWEET genes for creating disease-resistant cultivars utilizing completely different approaches, together with standard breeding and genome modifying.

TAL Effector Repertoires of Strains of Xanthomonas phaseoli pv. manihotis in Commercial Cassava Crops Reveal High Diversity on the Country Scale

Transcription activator-like effectors (TALEs) play a major position for pathogenesis in a number of xanthomonad pathosystems. Xanthomonas phaseoli pv. manihotis (Xpm), the causal agent of Cassava Bacterial Blight (CBB), makes use of TALEs to control host metabolism. Information about Xpm TALEs and their goal genes in cassava is scarce, however has been rising in the previous couple of years. We aimed to characterize the TALE variety in Colombian strains of Xpm and to display for TALE-targeted gene candidates.
We chosen eighteen Xpm strains based mostly on impartial genetic variety at a rustic scale to depict the TALE variety amongst isolates from cassava productive areas. RFLP evaluation confirmed that Xpm strains carry TALomes with a bimodal dimension distribution, and affinity-based clustering of the sequenced TALEs condensed this variability primarily into 5 clusters.
We report on the identification of 13 novel variants of TALEs in Xpm, in addition to a practical variant with 22 repeats that prompts the susceptibility gene MeSWEET10a, a beforehand reported goal of TAL20Xam668. Transcriptomics and EBE prediction analyses resulted in the number of a number of TALE-targeted candidate genes and two potential instances of practical convergence. This research gives new bases for assessing novel potential TALE targets in the Xpm-cassava interplay, which may very well be essential elements that outline the destiny of the an infection.
SWEET genes and TAL effectors for disease resistance in plants: Present status and future prospects

Consequences of adaptation of TAL effectors on host susceptibility to Xanthomonas

Transcription activator-like effectors (TALEs) are virulence elements of Xanthomonas that induce the expression of host susceptibility (S) genes by particularly binding to effector binding components (EBEs) in their promoter areas. The DNA binding specificity of TALEs is dictated by their tandem repeat areas, that are extremely variable between completely different TALEs. Mutation of the EBEs of S genes is being utilized as a key technique to generate resistant crops towards TALE-dependent pathogens. However, TALE variations via rearrangement of their repeat areas is a possible impediment for profitable implementation of this technique.
We investigated the results of TALE variations in the citrus pathogen Xanthomonas citri subsp. citri (Xcc), in which PthA4 is the TALE required for pathogenicity, whereas CsLOB1 is the corresponding susceptibility gene, on host resistance. Seven TALEs, containing two-to-nine mismatching-repeats to the EBEPthA4 that had been unable to induce CsLOB1 expression, had been launched into Xcc pthA4:Tn5 and adaptation was simulated by repeated inoculations into and isolations from candy orange for a period of 30 cycles.
While initially all strains failed to advertise disease, signs began to look between 9-28 passages in 4 TALEs, which initially harbored two-to-five mismatches. Sequence evaluation of tailored TALEs recognized deletions and mutations throughout the TALE repeat areas which enhanced putative affinity to the CsLOB1 promoter. Sequence analyses counsel that TALEs variations consequence from recombinations between repeats of the TALEs.
Reintroduction of those tailored TALEs into Xcc pthA4:Tn5 restored the flexibility to induce the expression of CsLOB1, promote disease signs and colonize host crops. TALEs harboring seven-to-nine mismatches had been unable to adapt to beat the incompatible interplay. Our research experimentally documented TALE variations to incompatible EBE and offered strategic steerage for era of disease resistant crops towards TALE-dependent pathogens.

TAL effector-like protein of an endofungal bacterium will increase the stress tolerance and alters the transcriptome of the host

Symbioses of micro organism with fungi have solely not too long ago been described and are poorly understood. In the symbiosis of Mycetohabitans (previously Burkholderiarhizoxinica with the fungus Rhizopus microsporus, bacterial kind III (T3) secretion is thought to be important. Proteins resembling T3-secreted transcription activator-like (TAL) effectors of plant pathogenic micro organism are encoded in the three sequenced Mycetohabitans spp. genomes. TAL effectors nuclear-localize in crops, the place they bind and activate genes essential in disease.
The Burkholderia TAL-like (Btl) proteins bind DNA however lack the N- and C-terminal areas, in which TAL effectors harbor their T3 and nuclear localization indicators, and activation area. We characterised a Btl protein, Btl19-13, and discovered that, regardless of the structural variations, it may be T3-secreted and can nuclear-localize. A btl19 -13 gene knockout didn’t forestall the bacterium from infecting the fungus, however the fungus turned much less tolerant to cell membrane stress.
Btl19-13 didn’t alter transcription in a plant-based reporter assay, however 15 R. microsporus genes had been differentially expressed in comparisons each of the fungus contaminated with the wild-type bacterium vs. the mutant and with the mutant vs. a complemented pressure. Southern blotting revealed btl genes in 14 various Mycetohabitans isolates.

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Description: A Rabbit polyclonal antibody against Mouse Ribonuclease Inhibitor (RI). This antibody is labeled with PE.

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Description: A Rabbit polyclonal antibody against Mouse Ribonuclease Inhibitor (RI). This antibody is labeled with APC.

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Description: A Rabbit polyclonal antibody against Mouse Ribonuclease Inhibitor (RI). This antibody is labeled with Cy3.

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Description: A Rabbit polyclonal antibody against Mouse Ribonuclease Inhibitor (RI). This antibody is labeled with HRP.

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Description: A Rabbit polyclonal antibody against Human, Rat Ribonuclease Inhibitor (RI)

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Description: A Rabbit polyclonal antibody against Mouse Ribonuclease Inhibitor (RI). This antibody is labeled with FITC.

Ribonuclease Inhibitor (RI) Polyclonal Antibody (Human, Mouse)

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Description: A Rabbit polyclonal antibody against Human, Mouse Ribonuclease Inhibitor (RI)

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Description: A Rabbit polyclonal antibody against Human, Rat Ribonuclease Inhibitor (RI). This antibody is labeled with PE.

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Description: A Rabbit polyclonal antibody against Human, Rat Ribonuclease Inhibitor (RI). This antibody is labeled with APC.

Ribonuclease Inhibitor (RI) Polyclonal Antibody (Human, Rat), Cy3

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Description: A Rabbit polyclonal antibody against Human, Rat Ribonuclease Inhibitor (RI). This antibody is labeled with Cy3.

Ribonuclease Inhibitor (RI) Polyclonal Antibody (Human, Rat), HRP

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Description: A Rabbit polyclonal antibody against Human, Rat Ribonuclease Inhibitor (RI). This antibody is labeled with HRP.

Ribonuclease Inhibitor (RI) Polyclonal Antibody (Human, Mouse), PE

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Description: A Rabbit polyclonal antibody against Human, Mouse Ribonuclease Inhibitor (RI). This antibody is labeled with PE.

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Description: A Rabbit polyclonal antibody against Human, Rat Ribonuclease Inhibitor (RI). This antibody is labeled with FITC.

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Description: A Rabbit polyclonal antibody against Mouse Ribonuclease Inhibitor (RI). This antibody is labeled with Biotin.

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Description: Recombinant Human Ribonuclease Inhibitor expressed in: E.coli

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Description: Recombinant Mouse Ribonuclease Inhibitor expressed in: E.coli

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Description: Recombinant Rat Ribonuclease Inhibitor expressed in: E.coli

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Description: A Rabbit polyclonal antibody against Human, Mouse Ribonuclease Inhibitor (RI). This antibody is labeled with APC.

Ribonuclease Inhibitor (RI) Polyclonal Antibody (Human, Mouse), Cy3

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Description: A Rabbit polyclonal antibody against Human, Mouse Ribonuclease Inhibitor (RI). This antibody is labeled with Cy3.

Ribonuclease Inhibitor (RI) Polyclonal Antibody (Human, Mouse), HRP

4-PAB444Hu01-HRP Cloud-Clone
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Description: A Rabbit polyclonal antibody against Human, Mouse Ribonuclease Inhibitor (RI). This antibody is labeled with HRP.

Ribonuclease Inhibitor (RI) Polyclonal Antibody (Mouse), APC-Cy7

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  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Ribonuclease Inhibitor (RI). This antibody is labeled with APC-Cy7.

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055225-01mL MyBiosource 0.1mL 260 EUR

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055225-02mL MyBiosource 0.2mL 335 EUR

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055225-05mL MyBiosource 0.5mL 630 EUR

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055225-1mL MyBiosource 1mL 770 EUR

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055225-5mL MyBiosource 5mL 2210 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055226-01mL MyBiosource 0.1mL 260 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055226-02mL MyBiosource 0.2mL 335 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055226-05mL MyBiosource 0.5mL 630 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055226-1mL MyBiosource 1mL 770 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055226-5mL MyBiosource 5mL 2210 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055228-01mL MyBiosource 0.1mL 195 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055228-02mL MyBiosource 0.2mL 245 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055228-05mL MyBiosource 0.5mL 430 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055228-1mL MyBiosource 1mL 520 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055228-5mL MyBiosource 5mL 1450 EUR

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055231-01mL MyBiosource 0.1mL 265 EUR

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055231-02mL MyBiosource 0.2mL 350 EUR

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055231-05mL MyBiosource 0.5mL 645 EUR

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055231-1mL MyBiosource 1mL 795 EUR

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055231-5mL MyBiosource 5mL 2275 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055232-01mL MyBiosource 0.1mL 265 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055232-02mL MyBiosource 0.2mL 350 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055232-05mL MyBiosource 0.5mL 645 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055232-1mL MyBiosource 1mL 795 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055232-5mL MyBiosource 5mL 2275 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055234-01mL MyBiosource 0.1mL 200 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055234-02mL MyBiosource 0.2mL 255 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055234-05mL MyBiosource 0.5mL 440 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055234-1mL MyBiosource 1mL 535 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055234-5mL MyBiosource 5mL 1495 EUR

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055237-01mL MyBiosource 0.1mL 275 EUR

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055237-02mL MyBiosource 0.2mL 365 EUR

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055237-05mL MyBiosource 0.5mL 675 EUR

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055237-1mL MyBiosource 1mL 835 EUR

APC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055237-5mL MyBiosource 5mL 2395 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055238-01mL MyBiosource 0.1mL 275 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055238-02mL MyBiosource 0.2mL 365 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055238-05mL MyBiosource 0.5mL 675 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055238-1mL MyBiosource 1mL 835 EUR

Cy3-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055238-5mL MyBiosource 5mL 2395 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055240-01mL MyBiosource 0.1mL 205 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055240-02mL MyBiosource 0.2mL 260 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055240-05mL MyBiosource 0.5mL 460 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055240-1mL MyBiosource 1mL 560 EUR

HRP-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055240-5mL MyBiosource 5mL 1570 EUR

Ribonuclease Inhibitor (RI) Polyclonal Antibody (Human, Mouse), FITC

4-PAB444Hu01-FITC Cloud-Clone
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Ribonuclease Inhibitor (RI). This antibody is labeled with FITC.

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055227-01mL MyBiosource 0.1mL 205 EUR

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055227-02mL MyBiosource 0.2mL 260 EUR

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055227-05mL MyBiosource 0.5mL 460 EUR

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055227-1mL MyBiosource 1mL 555 EUR

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055227-5mL MyBiosource 5mL 1560 EUR

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055233-01mL MyBiosource 0.1mL 215 EUR

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055233-02mL MyBiosource 0.2mL 265 EUR

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055233-05mL MyBiosource 0.5mL 470 EUR

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055233-1mL MyBiosource 1mL 575 EUR

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055233-5mL MyBiosource 5mL 1605 EUR

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055239-01mL MyBiosource 0.1mL 220 EUR

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055239-02mL MyBiosource 0.2mL 275 EUR

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055239-05mL MyBiosource 0.5mL 490 EUR

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055239-1mL MyBiosource 1mL 600 EUR

FITC-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2055239-5mL MyBiosource 5mL 1690 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093307-01mL MyBiosource 0.1mL 175 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093307-02mL MyBiosource 0.2mL 225 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093307-05mL MyBiosource 0.5mL 370 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093307-1mL MyBiosource 1mL 450 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093307-5mL MyBiosource 5mL 1235 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093308-01mL MyBiosource 0.1mL 180 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093308-02mL MyBiosource 0.2mL 225 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093308-05mL MyBiosource 0.5mL 380 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093308-1mL MyBiosource 1mL 465 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093308-5mL MyBiosource 5mL 1270 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093309-01mL MyBiosource 0.1mL 190 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093309-02mL MyBiosource 0.2mL 230 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093309-05mL MyBiosource 0.5mL 395 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093309-1mL MyBiosource 1mL 485 EUR

Biotin-Linked Polyclonal Antibody to Ribonuclease Inhibitor (RI)

MBS2093309-5mL MyBiosource 5mL 1335 EUR

Ribonuclease inhibitor Antibody

20-abx137320 Abbexa
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  • 100 ug
  • 20 ug
  • 5 ug

Rat Ribonuclease Inhibitor (RI) Protein

20-abx068925 Abbexa
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Ribonuclease Inhibitor (RI) Protein

abx068925-10g Abbexa 10 µg 425 EUR
However, banding patterns and accessible sequences counsel variation, and the btl19-13 phenotype couldn’t be rescued by a btl gene from a special pressure. Our findings assist the conclusion that Btl proteins are effectors that act on host DNA and play essential however diverse or probably host genotype-specific roles in the M. rhizoxinicaR. microsporus symbiosis.

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