Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting.

Loss of epithelial differentiation and gain of invasiveness correlates with tyrosine phosphorylation of the E-cadherin/beta-catenin complex in cells transformed with a temperature-sensitive v-SRC gene.

Loss of epithelial differentiation and gain of invasiveness correlates with tyrosine phosphorylation of the E-cadherin/beta-catenin complex in cells transformed with a temperature-sensitive v-SRC gene.

Loss of histotypic group of epithelial cells is a frequent characteristic in regular growth in addition to in the invasion of carcinomas. Here we present that the v-src oncogene is a potent effector of epithelial differentiation and invasiveness. MDCK epithelial cells transformed with a temperature-sensitive mutant of v-src exhibit a strictly epithelial phenotype at the nonpermissive temperature …

Loss of epithelial differentiation and gain of invasiveness correlates with tyrosine phosphorylation of the E-cadherin/beta-catenin complex in cells transformed with a temperature-sensitive v-SRC gene. Read More »

A simple cipher governs DNA recognition by TAL effectors.

A simple cipher governs DNA recognition by TAL effectors.

TAL effectors of plant pathogenic micro organism within the genus Xanthomonas bind host DNA and activate genes that contribute to illness or activate protection. Target specificity will depend on an effector-variable variety of sometimes 34 amino acid repeats, however the mechanism of recognition is just not understood. We present {that a} repeat-variable pair of residues specifies the nucleotides …

A simple cipher governs DNA recognition by TAL effectors. Read More »

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting.

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting.

TALENs are essential new instruments for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity is set by customizable arrays of polymorphic amino acid repeats within the TAL effectors. We current a way and reagents for effectively assembling TALEN constructs with custom repeat arrays. …

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Read More »

Highly sensitive ELISA for UV-induced DNA damage

The High Sensitivity CPD and the High Sensitivity (6-4) photoproducts ELISA from Cosmo Bio Most of the UV-induced DNA damage are cyclobutane pyrimidine dimers (CPDs) and (6-4) photoproducts (6-4PPs), which are formed between neighboring pyrimidine nucleotides on the same DNA strand. These helix-distorting DNA lesions are only repaired in humans by a nucleotide excision repair …

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What is genome and CRISPR-Cas9 editing?

Genome editing (also called gene editing) is a group of technologies that give scientists the ability to change the body’s DNA. These technologies allow the addition, removal or modification of genetic material at specific places in the genome. Several approaches to genome editing have been developed. The recent is known as CRISPR-Cas9, which is short …

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Co to jest edycja genomu i CRISPR-Cas9?

Edycja genomu (zwana również edycją genów) to grupa technologii, które dają naukowcom możliwość zmiany DNA organizmu. Technologie te umożliwiają dodawanie, usuwanie lub modyfikowanie materiału genetycznego w określonych miejscach w genomie. Opracowano kilka podejść do edycji genomu. Niedawny znany jest jako CRISPR-Cas9, który jest skrótem od grupowanych regularnie przerywanych krótkich powtórzeń palindromowych i białka powiązanego z …

Co to jest edycja genomu i CRISPR-Cas9? Read More »